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Easily Clone Unstable DNA
- Successfully clone unstable DNA
- Reduce rearrangement and deletion events
- Increase stability of DNA containing inverted repeats or Z-DNA
Eukaryotic DNA can contain irregular structures that makes it difficult
to clone in E. coli.
Replicating eukaryotic DNA accurately in prokaryotic cells can be
problematic. Eukaryotic DNA may contain inverted repeats or secondary
structures, such as Z-DNA, that can be rearranged or deleted by E. coli
DNA repair systems. Stratagene has solved this problem with our SURE®
competent cells for cloning unstable DNA.
Improved Cloning of Unstable DNA
Replicating eukaryotic DNA accurately in prokaryotic cells can be problematic.
Eukaryotic DNA may contain inverted repeats or secondary structures, such
as Z-DNA, that can be rearranged or deleted by E. coli DNA repair
systems. The SURE® series of competent cells are engineered to be
deficient in these repair systems and thus more stably maintain these
unstable DNAs. The UV repair system (uvrC) and the SOS repair pathway
(umuC) are both involved in repairing DNA lesions. Removal of genes in
these pathways results in a 10- to 20-fold increase in the stability of
DNA containing long inverted repeats. The E. coli proteins—the
products of the SbcC and RecJ genes—are involved in certain types
of recombination. Mutations in these genes greatly increase stability
of Z-DNA structures.
Figure 1
Cloning with SURE® Cells Dramatically
Improves the Percentage of Cells per Generation with Unrearranged
Plasmids
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Your Choice of Efficiency
SURE cells are available as electroporation-competent* (≥ 1 x 1010
transformants/µg DNA), as competent-grade** (≥ 5 x 108
transformants/µg DNA) or as a highly efficient derivative SURE 2
supercompetent cells*** (≥ 1 x 109 transformants/mg DNA).
* U.S. Patent Nos. 6,040,184 and 5,552,314 and patents
pending
** U.S. Patent No. 5,552,314 and patents pending
*** U.S. Patent Nos. 5,707,841, 5,552,314 and 5,512,468 and patents pending
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