Extend Your Research Possibilities
- Optimized for PCR Targets From 20kb to 50 kb in Length
- Amplifies Long complex Genomic Targets with Ease
- Higher Fidelity than Other Taq-Based DNA Polymerase Blends
Extremely long PCR templates present unique challenges, which
include the length of the target, complex secondary structures and lengthy
extension times.
EXL™ DNA polymerase*,‡ is a unique DNA
polymerase formulation that excels at amplifying extra long targets with
robust yield, specificity and higher fidelity than Taq DNA polymerase or
Taq-based blends. It successfully amplifies genomic targets up to 37 kb
and lambda targets up to
Specialized Formulation for Unique Challenges
The difficulty in amplifying extremely long genomic targets is not just
length alone. Secondary structures and repetitive sequences are common
within long DNA templates and can interfere with polymerization. Moreover,
exposing the DNA template and PCR reagents to lengthy extension times
required for amplifying long targets can limit PCR efficiency. EXL DNA
polymerase provides optimum performance in these extreme situations. It
successfully amplifies genomic targets up to 37 kb and lambda targets up
to approximately 50 kb. EXL DNA polymerase comes with an optimized PCR
buffer for extreme target lengths, a stabilizing solution and DMSO— which
together enable robust amplification of extremely long targets (Figure
1).
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Figure 1
EXL™ DNA Polymerase Excels at Amplifying
Extremely Long Targets
EXL™ DNA
polymerase easily amplifies two extremely long genomic targets (23
and 30 kb) and a lambda target (45 kb). A competitor's enqyme for
long targets was used for comparison. All reactions were performed
according to manufacturer's
recommendations. |
Greater Accuracy Means Greater Success
EXL DNA polymerase exhibits higher fidelity than Taq and other
Taq-based DNA polymerase blends. EXL is based on Pfu DNA
polymerase, the highest fidelity enzyme available, blended with Taq
DNA polymerase and other novel components to boost performance with
extremely long targets. The lower error rate (mutations per bp) of EXL DNA
polymerase ensures that fewer mistakes will be made during amplification
of long targets compared to PCRs carried out with Taq-based enzyme
formulations. EXL DNA polymerase’s superiorperformance in amplifying extra
long targets leads to greater success in downstream applications.
Contributing Scientists
Stratagene: Holly Hogrefe
and Mike Borns
* U.S. Patent Nos. 6,183,997 and 5,948,663 and 5,866,395
and 5,556,772 and 5,545,552 and patents pending.
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