You Can’t Clone Your DNA if It Can’t Get In
- 20- to 30-fold higher transformation efficiency of ligated DNA
- Ideal for transformation of large plasmid DNA
- Exhibits faster growth and bluer colonies
Transforming large plasmids with high efficiency is very difficult,
but even small plasmids can present the same problems because they are
much larger when ligated or relaxed instead of supercoiled.
Transforming large and ligated DNA can be difficult. That’s why
we created XL10-Gold® ultracompetent cells*.
These cells transform large and ligated constructs with high efficiency.
It’s the Hte phenotype that dramatically increases the transformation
efficiency of large plasmids.
High Transformation Efficiency
XL10-Gold® ultracompetent cells transform large plasmids and ligated
DNA with the highest transformation efficiency possible. The Hte phenotype
improves competent cell performance, as demonstrated in Figure 1. XL10-Gold,
XL2-Blue, and DH10B cells were transformed with several cDNA libraries
and the resulting colonies were counted. Compared to the other hosts,
XL10-Gold cells produced the most colonies, with 25-fold higher efficiency.
Large DNA
XL10-Gold cells are the only chemically competent cells that allow you
to efficiently transform large DNA molecules. Large DNA molecules include
big vectors, such as expression vectors, and big inserts, such as genomic
DNA. XL10-Gold ultracompetent cells are the host cells of choice when
you need the highest transformation efficiencies for large constructs.
Faster Growth
Another feature of the Hte phenotype is faster growth. Figure 2 shows
XL10-Gold cells exhibit more robust growth than XL2-Blue and DH5α
cells. When you are ready to select single colonies, you will have many
large colonies to choose from sooner than with other host cells.
*U.S. Patent Nos. 5,512,468 and 5,707,841 and patents pending.
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