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A Simple Method To Sequence From Bacterial Colonies Using [a-33P] Radiolabeled DdNTPs And Thermo Sequenase

Affymetrix / USB

Tuesday, April 18, 2006
In traditional DNA sequencing systems, sequencing products are radioactively labeled using either endlabeled primers or by the incorporation of ... read more
USB® OptiKinase™ Eliminates Base Bias In Labeling Of Oligonucleotide 5'-Ends

Affymetrix / USB

Tuesday, April 18, 2006
Wild-type T4 Polynucleotide Kinase (PNK)(1,2,3) is commonly used for phosphorylation and labeling of 5'-ends of DNA and oligonucleotides. Labeling, in... read more
Simple Approaches For Optimization Of RT-PCR

Affymetrix / USB

Tuesday, April 18, 2006
Reverse transcription-polymerase chain reaction (RT-PCR) converts and amplifies a single-stranded RNA template to yield abundant double-stranded DNA ... read more

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Serum-free Media for the Maintenance of Sf9 Cells

Our lab uses serum-free and supplemented SF-900 II media for the maintenance of Sf9 cells in suspension. Serum-free media is used during transfection whereas ... read more

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