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Date Published
Title
The Use Of TaqMaster PCR Enhancer ®
Eppendorf North America
Tuesday, April 08, 2003
Transgenic mice carry a fragment of foreign DNA that has been incorporated into their genome. The most common method of introducing ...
read more
The Use Of MasterTaq® Polymerase For PCR With Humic Material-Contaminated DNA
Eppendorf North America
Tuesday, April 08, 2003
Direct extraction of total DNA from soils or sediments always results in coextraction of other soil components, mainly humic acids or other ...
read more
Simple And Rapid Isolation Of Plant RNA Using The Eppendorf
®
Perfect RNA, Eukaryotic, Maxi Kit
Eppendorf North America
Tuesday, April 08, 2003
The isolation of plant RNA is a particularly challenging task. There are many protocols for the isolation of total RNA from plants that ...
read more
Normalizing CDNA Libraries Using The Eppendorf®Thermomixer
Eppendorf North America
Tuesday, April 08, 2003
During many experiments in molecular biology, the individual mRNA expression pattern of a cell is examined in order to investigate the ...
read more
Optimizing The Direct Amplification Of Missing CDNA 5' Ends Using The Eppendorf® Mastercycler® Gradient
Eppendorf North America
Tuesday, April 08, 2003
In many research projects, cDNA isolation forms the basis for detailed analyses at the molecular level. However, when cDNA libraries are ...
read more
Optimized Dengue RT-PCR
Eppendorf North America
Tuesday, April 08, 2003
Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR*) for dengue is often carried out at the Institute of Health and Community Medicine, ...
read more
Optimizing DNA Amplification Protocols Using The Eppendorf® Mastercycler®
Eppendorf North America
Tuesday, April 08, 2003
The Polymerase Chain Reaction (PCR*) is a molecular-biological process that, during recent years, has been developed into a method used in ...
read more
Amplification Of Epstein-Barr Virus Exon C With Difficult Primers Using Eppendorf® Mastercycler® Gradient
Eppendorf North America
Tuesday, April 08, 2003
Epstein-Barr virus (EBV) is associated with various types of malignancy, in particular, nasopharyngeal carcinoma (NPC). The viral oncogene, ...
read more
Using Gradient PCR To Determine The Optimum Annealing Temperature
Eppendorf North America
Tuesday, April 08, 2003
Problems with the amplification of a specific DNA fragment using the PCR* are an everyday occurrence in the lab. For example, ...
read more
The Use Of The Mastercycler ® Gradient For Single-cell PCR And Preimplantation Diagnosis
Eppendorf North America
Tuesday, April 08, 2003
Preimplantation diagnosis (PGD) is a very early form of prenatal diagnosis: embryos obtained in vitro from couples at risk of contracting ...
read more
Optimizing The Direct Amplification Of Missing CDNA 5' Ends Using The Eppendorf® Mastercycler® Gradient
Eppendorf North America
Tuesday, April 08, 2003
In many research projects, cDNA isolation forms the basis for detailed analyses at the molecular level. However, when cDNA libraries are ...
read more
Two-dimensional Optimization Of A Semi-nested PCR For Detecting Listeria Monocytogenes
Eppendorf North America
Tuesday, April 08, 2003
Bacterial enteritides, which are triggered by contaminated foodstuffs, pose a significant health risk for consumers. The legally prescribed ...
read more
PCR Optimization
Eppendorf North America
Tuesday, April 08, 2003
There is obviously no single set of conditions that can be applied to all PCR amplifications. For example, depending on the length and...
read more
General Notes On Primer Design In PCR*
Eppendorf North America
Tuesday, April 08, 2003
Perhaps the most critical parameter for successful PCR is the design of Primers. All things being equal, a poorly designed primer can ...
read more
Two-step Gradient PCR*
Eppendorf North America
Tuesday, April 08, 2003
Science underwent revolutionary changes during the 1980's, particularly in the field of genetics. One of the most significant changes was ...
read more
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Anti-human HLA-A2 Antibody
BB7.2 is an example of a polymorphic antibody that recognizes epitopes carried by subsets of HLA class I variants. The epitope recognized by BB7.2 lies in the ...
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