MagSi Beads & Solutions for Proteomics Applications

MagSi Beads & Solutions for Proteomics Applications

Examples of beads used in protein isolation and proteomics

• MagSi-protein G bead type performance and competition
• MagSi-proteomics performance and competition
• MagSi-WCX and MagSi-WAX
• MagSi-S plain silica bead stability

Capacity of MagSi-protein G beads

• GAPDH was used as model
• GAPDH was bound and eluted, analyzed by SDS PAGE

Results show:

Lane 1: MagSi-protein G 600
Lane 2: MagSi-protein G 1.0
Lane 4: Comp. Dynal protein G

MagSi proteomics C18 beads Manual Handling testing results

• 4 – 8 fold higher sensitivity analyzing the BSA digest compoared to Microcolumn and ZipTip desalting (right hand site a.u.)
• Significantly more peptides could be annotated/identified (numbered peaks)
• Best signal to noise ratio
• Enhancement of magnetic capture can add additional performance (use 1 µm to 2 µm beads).

MagSi-proteomics beads for bodyfluidics testing – here serum as sample

Serum sample – the various MagSi beads are adressing the various targets

MagSi proteomics C18 beads Automated Handling testing results

• Equipment: Mikrotiterplate (MTP) format for use in automated (robotic) systems, (Tecan)
• 24 peptide hits using MagSi beads (vs 8 and 20 hits using competitors beads).
• Complex saliva sample
• Therefore optimized organic solvents were used for MTP use.

Guideline: Choose the right beads for the right application

Alternative applications:

• MagSi-WAX/WCX: suitable in phosphoproteomics as prefractionation/depletion tool
• MagSi-proteomics C4, C8 and C18: concentration of diluted samples, e.g Secreted proteins/peptides into media
• MagSi-protein A/G: capture/enrichment/release of phosphorylated protein/peptides

Replacing HPLC fractionation with MagSi-proteomics beads

Stability of MagSi beads

• MagSi-S beads have been tested under high salt conditions, high temperature and both
• MagSi-S beads are stable for pH values between 3 and 13
• MagSi-S beads are stable for temperatures up to 95°C
• MagSi-S beads are stable in high salt conditions up to 4M NaCl

Customization Potential for Particles & Beads

MagSicustom product development potential

Customization Potential for Particles & Beads...when standard doen't fit

Speed and versatilaty of the manufacturing process enable us to offer beside ready to use products tailor-made particles with a wide range of features and short term developement cycles

Conclusions

• AMSBIO provides the complete portfolio for biochromatographie, mass spec sample prep. and antibody based applications
• Beads on client demands (MagSi custom) are available in short time frames and scalable batch sizes
• MagSi-S core beads are physically stable even under harsh conditions