Highly Reproducible Gene Delivery for Stem Cell Research
AMSBIO have announced a range of ready-touse
lentivirus supernatant products suitable
for many kinds of gene delivery applications including mammalian protein expression, stable
cell line construction, cell signal pathway
localization and stem cell research.
Converting fully differentiated mouse or human
somatic cells into embryonic-like cells (so called
induced Pluripotent Stem Cell: iPSC) has
attracted enormous attention in stem cell
research. Multiple reports have demonstrated that
iPS cells were generated by using a set of
transcription factors or stem cell factors that
delivered as expression virus or expressed
proteins. Although the combination of
reprogramming factors may slightly different, the
main stem cell factors are: OCT3/4, SOX2,
NANOG, LIN28, c-Myc and KLF4. iPSC holds the
promise of curing many human diseases and
accelerates the stem cell research.
Using the SureTiter™ lentiviral system from
AMSBIO, you can generate high-titer lentiviral
particles for all six human stem cell factors. Each
factor was fully sequencing verified and matched
to the CDs in NCBI database. High titer lentiviral
particles / supernatant were produced in 293T
packaging cells in DMEM with 10% heatinactivated
FBS. They are psudotyped with VSV-G
glycoprotein-attached vector map.
Prepared using proprietary protocols to integrate
a real-time fluorescence monitoring tag in the
system - each 200 µl vial of AMSBIO lentivirus
supernatent contains a
high titre of highly transducible lentivirus (1 x 10
e7 IFU/ml). Each vial of lentiviral supernatent
particles contains a fully sequence verified target,
ready for transduction into any mammalian cells.
All AMSBIO lentivirus supernatent products are
easy and safe to use, simply add 50 µl into the
cultured cells, and you will be able to confirm the
specific target's expression under a fluorescent
microscope after 48-72 hours.
The AMSBIO lentiviral system is a gene delivery
tool using lentivectors for gene expression or
knockdown. Lentivirus can effectively transduce
both dividing and non-dividing mammalian cells,
and integrate into the host genome, allowing
stable long-term, high-level gene expression both
in vivo and in vitro. Unlike traditional retroviral
system, AMSBIO lentivirus is much more actively
imported into the nuclei of non-dividing cells and
stably integrated into the host cell's genome
independent of cell cycle. Although adenovirus is
also able to transduce non-dividing cells, it is only
for transient expression because it cannot
intgrate into host cell's genome.