Kits for improved nucleic acid recovery from FFPE samples
AMSBIO has announced new kits for the
isolation of high quality RNA from Formalin-
Fixed Paraffin-Embedded (FFPE) tissues and
LCM samples that can be applied both to
qRT-PCR and a variety of microarray
platforms.
When working with stored materials, researchers
often need to isolate RNA from samples that
have been stored as FFPE tissue blocks. These
FFPE tissues are a valuable source of information
for retrospective research. Although the tissue
structure of FFPE samples will have been
maintained for histological analysis, damage to
the nucleic acid may have occurred through the
fixation, embedding, and storage processes
impeding measurements of gene expression
levels. The degree of RNA fragmentation that
typically has already occurred in FFPE tissues
cannot be reversed, so researchers are faced
with the challenge of optimizing RNA gene
expression analysis protocols when working with
these samples.
ExpressArt® FFPE RNAready overcomes the
limitations associated with RNA degradation and
interference from RNA cross-linking in FFPE
tissues. The FFPE RNAready kit efficiently isolates
and preserves mRNA utilizing a specially
developed lysis solution and an innovative
universal inhibitor of RNases that displaces
tightly bound RNA from high-molecular-weight
aggregates. Subsequent treatment with a unique
de-modification reagent reverses formalin
induced cross-links, resulting in total RNA optimized for reverse transcription reactions and
subsequent downstream applications.
FFPE RNAready together with ExpressArt® RNA
amplification TRinucleotide primer technology is
now available to amplify microgram quantities of
high quality RNA from very small amounts of
degraded total RNA, with minimal loss of
sequence.