Upgraded MagSi products can seamlessly
replace discontinued Dynabeads®
MagnaMedics Diagnostics B.V. have developed a high magnetic content
(HMC) bead with sizes of 0.6 μm and 1.0 μm (1.2 μm
for MagSiproteomics and MagSi-DNA allround beads) to replace all
previous MagSi products. The use of a novel, newly developed ferro
fluid (Mag-Ferro) with an magnetisation of up to 40 emu/g raises the
magnetic content from ~30% to 50% enhancing the time to magnet
significantly (Figure 1).
Furthermore, Invitrogen™ has recently announced that it is to
discontinue Dynabeads® for proteomic applications.
MagSi products can
seamlessly replace these discontinued product lines and many other
types of Dynabead without major changes in protocols or handling.
MagSi has a proven track record for manual use and robotic
platforms (Beckman Coulter®, Tecan®, IDS®, Xyril®, Stratek®,
Hamilton®, Roche® and others)
The advantages of the MagSi Products after the upgrade:
- 4-6 times faster and a more complete collection of the MagSi
beads.
- Small bead with the magnetic performance and strength of a
largerLower carry-over of contaminants.
- adjustment) even with highly viscous solvents and samples.
- Higher yield of your target biomolecule, especially in assays
with complex binding kinetics.
- Better reproducibility.
- Small bead with the magnetic performance and strength of a larger
version.
- Same size distribution, mean size, surface functionality (e.g.
binding capacity, sensitivity) and sedimentation time as the old
beads
MagSi-proteomics beads are
magnetic beads that are an ideal tool for the purification,
concentration and desalting of peptides and protein digests. The
surface of the beads has been modified with C4, C8 and C18 -alkyl
groups that are typical for reversed phase applications.
The MagSi-WCX
(weak cation exchange) magnetic beads have typical ion exchange
properties well known from classical chromatography. Protein and
peptides are charged at the surface and will be adsorbed to magnetic
beads under low salt condition. Under high salt conditions or by a pH
shift the target proteins/peptides are eluted (ion exchanged). Due to
the different binding/elution mechanisms the MagSi-WCX beads are a
powerful alternative to the MagSi proteomics beads.
The MagSi-WAX (weak anion exchange) magnetic beads
are the “counter” beads to the MagSi WCX beads. Binding,
washing and elution are also triggered by the protein/peptide total net
charge. The MagSi-WAX beads are ideally suited when more acidic
proteins/peptides are expected in the sample. However, our protocols
given in the corresponding product sheet cover buffer systems for
acidic, neutral and basic conditions.
MagSi-WCX beads are magnetic silica beads with a
high magnetic content optimized for protein and peptide separation.
Since the MagSi-WCX and MagSi-WAX act as counter-beads, we recommend
that both types of beads are tested, to see which suits individual
applications best.
MagSi
/ Invitrogen™ (Dynal™) Product Replacement Guide Product table
Notes:
MagSi beads are also available as 600 nm particles for increased binding capacity and sensitivity and
in higher standard volumes
* Discontinued by Invitrogen™
** Please also see the MagSi protein and peptide and MagSi proteomics selection guide
*** MagSi 1.0 μm beads can replace InvitrogenTM 2.8 μm and 1 μm beads in most existing
applications