Cleanascite™ a Delipidation Reagent for Mycobacterium tuberculosis Samples in RNA Research.

Cleanascite™ a delipidation reagent for Mycobacterium tuberculosis samples in RNA research.

Scientists from the Pacific Tuberculosis and Cancer Research Organization have cited Cleanascite™ for delipidation during RNA isolation in “Functional Analysis of Intergenic Regions for Gene Discovery”. Samples of M. tuberculosis strain H37Rv underwent bacterial lysis. RNA isolation was performed by mixing cultures in equal volumes of RNA,bacteria collected by centrifugation (1 min, 25000g, 8°C) and the mycobacteria were mechanically disrupted. Cleanascite™ from Biotech Support Group was used for delipidation of aqueous phase lipids followed by extraction using chloroform- isoamyl alcohol, ethanol precipitation.

Cleanascite™ is used for lipid removal in sample preparation. Cleanascite™ removes lipids from cells, biological fluids, and tissues. The protocol is easy to automate using a conventional micropipetting robot, allowing for high-throughput profiling of complex samples. The advantage of Cleanascite™ lipid removal over conventional two-phase chloroform- containing solvent systems is nontoxic, noncarcinogenic and easy to use. Cleanascite is noncorrosive and chemically stable, forms no peroxides during storage. Cleanascite™ is a solid-phase, non-ionic absorbent that extends the life of membrane and chromatographic columns.

Cleanascite™ is ideal for delipidation of protein and RNA samples. Scientists developed a method for gene finding in intergenic regions of annotated genome which integrated transcription analysis, sequence alignment and homology. Microarray based transcriptional analysis for functional gene finding allowed researchers to discover genes with specific functions such as DNA-binding proteins in CopG family and nickel binding GTPase and figure out functional domains of proteins in active intergenic elements with significant transcription activity. Cleanascite™ is very efficient for the removing plasma lipids, while sparing the protein fraction for further use . When proteins are studied by electrophoresis, lipids should be removed to obtain good resolution.

CleanasciteTMReferences:

• Li M. Fu (2011). Functional Analysis of Intergenic Regions for Gene Discovery, Computational Biology and Applied Bioinformatics, Heitor Silverio Lopes and Leonardo Magalhães Cruz (Ed.), ISBN: 978-953-307-629-4, InTech.