Cleanascite™ is a lipid removal reagent from serum free media for efficient isolation of large scale antibody-secreting hybridomas

Cleanascite™ is a lipid removal reagent from serum free media for efficient isolation of large scale antibody-secreting hybridomas

Cleanascite ™ from Biotech Support Group removes lipids from samples containing hybridoma cultures & thus is suitable for samples of serum-free media used in hybridoma growth and monoclonal antibody purification. Lipid removal of cytoplasmic lipid droplets and lipoproteins is desired prior to protein purification to decrease nonspecific contamination of downstream processing. Cleanascite is ideal for delipidation treatments for downstream processing of large-scale therapeutic proteins, enzymes and monclonal antibodies. The serum free media contains lots of lipids but to purify the samples from lipids a lipid removal reagent should be used and Cleanascite is the #1 lipid removal reagent.

Because of the lipid rich nature of serum-free media, this medium is suitable for high cell growth of serum supplemented cultures. Researchers doing experiments for growing specific cell types or doing research in the absence of serum frequently use serum-free media (SFM) because it allows for strict set of conditions that are free of confounding variables. A major concern in the manufacturing process of biopharmaceuticals is the viral, bacterial and fungal contamination. Serum-free media are highly purified, have a low risk of contamination and is commercially available and is a routinely used reagent in many laboratories for the culture of cell types in drug discovery, physiological, or gene expression studies.

• After lipids, cell debris, lipoproteins, floating fats, cohn paste, transgenic milk, egg yolk and mucinous impurities from biological samples are removed via Cleanasicte™’s unique technology, measuring cellular function and detection of cellular mediators is possible.
• Cleanascite ™ from Biotech Support Group offers a rapid implementation method with reproducible and consistent results that ensure clarity of samples and compatibility of downstream processing.

• For example, a hormone-supplemented, lipid-enriched serum-free medium, which grows NS-1 mouse myeloma cells is a standard sample used for isolation of antibody-producing hybridomas formed from the fusion of NS-1 myeloma cells and spleen cells from immunized mice. Cleanascite ™ after removing lipids allows for probably more efficient isolation of antibody-secreting hybridomas and a higher yield of antibody-producing hybridomas.
• Also, bovine embryos developed from in vitro-matured (IVM) and -fertilized (IVF) oocytes cultured in either serum-free or serum-containing media that contain lipids would be analyzed easily after using Cleanascite ™.
• Cleanascite is used for delipidation studies. Cleanascite removes lipids after making hybridoma monoclonal antibody and it was successfully used in US Patent 20030170757 (Application number: 10/373,579). The sample for this US Patent is mouse ascites containing MAB directed to human serum in tumor cell specific NADH: protein thiol reducatase and hybridoma cell lines.

References:

1. Lipid Removal from Human Serum Samples Castro, Arnold R., Morrill, William E., Pope, Victoria Clin. Diagn. Lab. Immunol. 2000 7: 197-199
2. Increased levels of beta 2 glycoprotein I antigen and beta 2 glycoprotein I binding antibodies are associated with a history of thromboembolic complications in patients with SLE and primary antiphospholipid syndrome. McNally T, Mackie IJ, Machin SJ, et al.Br J Rheumatol. 1995;34:1031–6. 
3. Kovár J, Franĕk F. Serum-free medium for hybridoma and parental myeloma cell cultivation.Methods Enzymol. 1986;121:277–292. [PubMed]
4. Tharakan JP, Lucas A, Chau PC. Hybridoma growth and antibody secretion in serum-supplemented and low protein serum-free media. J Immunol Methods. 1986 Nov 20;94(1-2):225–235.
5.   Kawamoto T, Sato JD, Le A, McClure DB, Sato GH. Development of a serum-free medium for growth of NS-1 mouse myeloma cells and its application to the isolation of NS-1 hybridomas.Anal Biochem. 1983 Apr 15;130(2):445–453. [PubMed]
6. Cleveland WL, Wood I, Erlanger BF. Routine large-scale production of monoclonal antibodies in a protein-free culture medium. J Immunol Methods. 1983 Jan 28;56(2):221–234