BindPro ™ Metabolomics Allows For Enrichment of Metabolites/Analytes By Removing Proteins From Serum or Plasma For Metabolomics Research.

BindPro ™ Metabolomics Allows For Enrichment of Metabolites/Analytes By Removing Proteins From Serum or Plasma For Metabolomics Research.

For the determination of metabolites from human serum and plasma, sample preparation is necessary before metabolites/analytes are detected by MS/GC/LC/NMR. Because proteins interfere with metabolite/analytes analysis, it is important to remove proteins and enrich metabolites/analytes using BindPro™ Metabolomics. BindPro™ Metabolomics has higher efficiency compared to other methods like chemical precipitation or membrane filtration because BindPro™ Metabolomics lacks the disadvantages of cross contamination and shear damage. It is ideal for analytes that are water soluble. Consequently, BindPro™ Metabolomics has applications in a range of drug binding, screening(toxicology, transplant monitoring) and metabolomic research (protein-metabolite interactions, biomarker discovery, and functional annotation of both proteins and metabolites).

BindPro™ Metabolomics allows you to enrich metabolites or analytes by removing proteins from the serum or plasma samples. After using BindPro™ Metabolomics, the analysis of metabolite/analyte data profiles using MS/GC/LC/NMR is beneficial for understanding the close biological associations of metabolites/analytes. Some examples of metabolites/analytes present in biological samples are sodium, sulfur, magnesium, phosphorus, phosphates, potassium, iron, oxygen, bicarbonate, L-alanine, carbon dioxide, chlorine, L-lysine, fructosamine, calcium, urea, melanin, D-glucose, total cholesterol, ATP, glyceraldehyde , cholesterol esters, methanol, glycine, uric acid, and (R)-3-hydroxybutyric acid.

Research publications have shown that serum and plasma samples are ideal for metabolomics research. After using BindPro™ Metabolomics for protein removal, serum and plasma samples are compatible for downstream applications such as MS/GC/LC/NMR that enable metabolomics research. BindPro™ Metabolomics has an efficacious extraction technique for performing large-scale, highthroughput protein removal from serum or plasma samples.

Published Articles for BindPro™

Wolbachia Lipoprotein Stimulates Innate and Adaptive Immunity through Toll-like Receptors 2 and 6 to Induce Disease Manifestations of Filariasis

BindPro™ can eliminate the artifacts of protein contamination and improve study of amino acids, sugars, lipids, nucleotides, and other biomolecules from biological tissues and fluids. In this article, researchers used Cleanascite™ first to determine if TLR2/6 ligands of Wolbachia are lipoproteins for removing lipids and lipoproteins. Next BindPro™, a polymeric protein removal suspension reagent (Biotech Support Group)was used to ablate levels of HEK-TLR2 cell IL-8 reporter gene activity to BMFE thereby showing that the TLR2/6 activity depends on both lipid and protein moieties.

For more information: http://biotechsupportgroup.com/bindpro
Applications of BindPro™: http://biotechsupportgroup.com/applications/bindpro%E2%84%A2-applications

References for BindPro™:

1. Wolbachia Lipoprotein Stimulates Innate and Adaptive Immunity through Toll-like Receptors 2 and 6 to Induce Disease Manifestations of Filariasis Joseph D. Turner J. Biol. Chem. 2009 284: 22364-22378.

Metabolomics Research References:

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