Wednesday, November 09, 2005
Isopure™ - Buccal cell DNA isolation
The Isopure™ Buccal Cell DNA Kit is a simple, streamlined method
for isolating cheek buccal cell genomic DNA for downstream applications
such as PCR. The kit supports swab or Scope
® mouthwash
collection formats using SPRI
® magnetic bead technology
that eliminates the need for filtration or centrifugation. The Isopure
Buccal Kit effectively and consistently delivers high purity and yield.
The method can be run in tube or 96-well formats and can be easily automated
for multi-microplate processing.
KEY FEATURES:
• Supports swab and mouthwash formats
• Easy-to-use protocol
• High recovery of DNA material
• Supports tube, 96-well or multi-microplate automation
EASY-TO-USE AND AUTOMATION COMPATIBLE
The Isopure Buccal Cell DNA Kit utilizes the power of SPRI (Solid Phase
Reversible Immobilization)
magnetic bead technology that allows for scalability, automation compatibility
and a streamlined protocol.
There is no need for filtration, centrifugation, use of chaotropic salts
or corrosive detergents. SPRI beads
also easily stay suspended in solution and do not catalyze precipitation.
SUPERIOR MICROPARTICLES – SUPERIOR PURIFICATIONS
The superior performance of SPRI is the result of the unique properties
of the chemistry and the highlyengineered
Sera-Mag microparticles manufactured by Seradyn. A proprietary core-shell
manufacturing process
results in complete iron encapsulation and highly uniform bead size. The
non-silica, carboxylate-modified
coating overcomes the limitations of silica-based purifications, such
as high non-specific binding. The fast
magnetic response time, extremely slow settling rates, and a high-surface
area to mass ratio of the Sera-Mag
microparticles make SPRI cost effective, highly efficient and automation-friendly.
RECOVERY AND YIELD:
The Isopure Buccal Cell method consistently delivers
sufficient material to carry out several downstream
PCR experiments.Yields can range from 50 to
1,000 ng depending on which collection method
is employed.The Scope mouthwash method
showed linear recovery in tests from 150-450 µL
of starting source material.
ORDERING INFORMATION:
For ordering or product information please visit
our website at www.agencourt.com
or call:
US or Canada: +1-800-361-7780
International: +1-978-867-2603
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