FULL LENGTH INSERT SEQUENCING™
Agencourt offers a full length insert sequencing
service for large insert vectors such as BACs, cosmids and fosmids. Agencourt
has several features that allow us to provide high quality results with
rapid turnaround including:
Long Phred20 read lengths
Unique library construction strategies
Patented SPRI™ technology
Oracle-based Galaxy LIMS™
NHGRI funded, GLP-compliant facility
FULL LENGTH INSERT SEQUENCING SERVICE
Sequence reaction set up and thermocycling
Sequence delineation on an
ABI PRISM 3700/3730xl
Assembly and finishing
Data delivery via a secure FTP site
FULLY-AUTOMATED, GLP-COMPLIANT FACILITY
Agencourt has a fully-automated genomic services pipeline based on SPRI,
our patented nucleic acid purification technology. All aspects of our pipeline
are managed by our Galaxy LIMS (Laboratory Information Management System).
Agencourt’s proactive approach to quality management provides our clients
confidence in the quality and integrity of their sequence data.
FINISHING AND ASSEMBLY
Our quality standards are among the highest in the industry, with error
rates well below 1 in 10,000 nucleotides in the assembled contigs. Our finishing
strategies can include custom primer walking, transposon insertion and/or
direct PCR sequencing. We use both commercial and proprietary software for
semi-automated finishing and assembly including Phred, PHRAP and GAP 4.
We also use paired end sequence information to resolve repeat sequences
and maximize the level of contig ordering. Customers receive finished contigs
annotated with quality scores for each base.
NHGRI SEQUENCE PROVIDER
The NHGRI (National Human Genome Research Institute) recognized Agencourt’s
sequence quality and large-scale data management capabilities by naming
Agencourt as one of NIH’s Large Sequencing Centers. To date, Agencourt has
taken part in numerous whole genome sequencing efforts including the Human,
Mouse, Tetraodon and Rickettsia Genome Projects and will continue large-scale
genome projects as a sequence provider for NHGRI.