Full Length Insert Sequencing™

FULL LENGTH INSERT SEQUENCING™

Agencourt offers a full length insert sequencing service for large insert vectors such as BACs, cosmids and fosmids. Agencourt has several features that allow us to provide high quality results with rapid turnaround including:

Long Phred20 read lengths

Unique library construction strategies

Patented SPRI™ technology

Oracle-based Galaxy LIMS™

NHGRI funded, GLP-compliant facility

FULL LENGTH INSERT SEQUENCING SERVICE

Library construction

Template purification

Sequence reaction set up and thermocycling

Dye-terminator removal

Sequence delineation on an

ABI PRISM 3700/3730xl

Assembly and finishing

Data delivery via a secure FTP site

FULLY-AUTOMATED, GLP-COMPLIANT FACILITY
Agencourt has a fully-automated genomic services pipeline based on SPRI, our patented nucleic acid purification technology. All aspects of our pipeline are managed by our Galaxy LIMS (Laboratory Information Management System). Agencourt’s proactive approach to quality management provides our clients confidence in the quality and integrity of their sequence data.



FINISHING AND ASSEMBLY
Our quality standards are among the highest in the industry, with error rates well below 1 in 10,000 nucleotides in the assembled contigs. Our finishing strategies can include custom primer walking, transposon insertion and/or direct PCR sequencing. We use both commercial and proprietary software for semi-automated finishing and assembly including Phred, PHRAP and GAP 4. We also use paired end sequence information to resolve repeat sequences and maximize the level of contig ordering. Customers receive finished contigs annotated with quality scores for each base.

NHGRI SEQUENCE PROVIDER
The NHGRI (National Human Genome Research Institute) recognized Agencourt’s sequence quality and large-scale data management capabilities by naming Agencourt as one of NIH’s Large Sequencing Centers. To date, Agencourt has taken part in numerous whole genome sequencing efforts including the Human, Mouse, Tetraodon and Rickettsia Genome Projects and will continue large-scale genome projects as a sequence provider for NHGRI.