Sunday, February 19, 2012
SensoLyte® α-Synuclein ELISA Assay – Ultrasensitive
AnaSpec, EGT Group is pleased to announce the release of an ultrasensitive ELISA assay for quantitatingα-synuclein. The SensoLyte® α-Synuclein Quantitative ELISA Kit (Human/Mouse/Rat) provides a convenient and quantitative assay for determining α-Synuclein amount in cell and tissue lysate as well as in body fluids. Compared to other anti-α-Synuclein ELISA kits on the market, this one-step, mix-and-read assay takes less time to run. HRP conjugated detection antibody is added simultaneously with the samples and standards during the assay, eliminating extra incubation and washing steps.
Product
Catalog
#
SensoLyte®
Anti-α-Synuclein (Human, Mouse, Rat) ELISA Kit
55550
§ One Step Convenient Format
- Pre-coated and pre-blocked 96-well strip plate
- Ready-to-use substrate solution and other assay components
- One step assay (samples and detection antibody are added simultaneously)
- 1 hour assay time at room temperature (excluding incubation time)
§ Minimal Sample Size
- Requires only 10-20 μl of cell and tissue lysate or body fluids
§ High Sensitivity
- Detects 5 pg/ml of α-Synuclein (calculated as three standard deviations from the blank)
§ Broad Dynamic Range
- 8-500 pg α-Synuclein/ml in the assayed sample
Figure 1. An example of the α-Synuclein ELISA standard curve
α-Synuclein is a major component of Lewy bodies found in affected neurons of patients with Parkinson’s disease.1-4 This protein has a mass of 14.5 kDa (140 amino acids long) and consists of a conserved degenerative amino-terminal domain and an acidic carboxyl-terminal that has a higher sequence divergence.1-4 α-Synuclein is predominantly expressed in brain, specifically in cerebellum, thalamus, neocortex, hippocampus, and striatum regions.1-2 Other tissues also express α-Synuclein but at very low levels.1-4 The physiological role of α-Synuclein is not well understood. However, the presence of imperfect KTKEGV lipid interacting repeats suggests that it may be involved in synaptic vesicle homeostasis.3
Related
Products
Table 1. A listing of Synuclein recombinant proteins
Product
Size
Catalog
#
Recombinant
human α - Synuclein (1 - 140)
500
µg
1000
µg
55456-500
55456-1000
Recombinant
human α - Synuclein (1 - 140), biotin labeled
200
µg
55581
Recombinant
human α - Synuclein (1 - 140), HiLyte Fluor™ 488 labeled
200
µg
55457
Recombinant
Human β - Synuclein (1 - 134)
100
µg
500
µg
1000
µg
55458-100
55458-500
55458-1000
Table 2. A listing of Synuclein antibodies.
Product
Size
Catalog
#
Anti-α-Synuclein
(mouse)
50
µg
53877
Anti-α-Synuclein
(pSer 87)
25
µg
50
µg
55329-025
55329
Anti-α-Synuclein
(paired 87)
50
µg
55330
Anti-Synuclein
(pan)(IN)
50
µg
55916
Anti-ß-Synuclein
50
µg
53879
References:
- Rivers, R. et al. Protein Science 17, 887 (2008).
- Bruening, W. et al. Cancer 88, 2154 (2000).
- George M. J. Genome Biology3, 1 (2001).
- Latawiec, D. et al. PloS ONE 5, 1 (2010).