[Me2s] & [Me2a]-Histone Peptides
AnaSpec, EGT Group offers one of the world’s largest selections of Histone GO® (catalog) peptides. Our peptides include sequences containing modified amino acids such as methylated (Me1, Me2 and Me3) Lysine; phosphorylated Threonine (pThr) or Serine (pSer); deiminated Arginine (Cit). We also offer symmetric and asymmetric dimethylated Arginine (Me2s and Me2a) in addition to monomethylated Arginine (Me1).
Methylation of arginine residues are mediated by members of the Protein arginine methyltransferases (PRMTs) family. PRMT type I adds asymmetrically dimethyl groups to arginine (ADMA), while type II add symmetrically dimethyl groups (SDMA). Type III adds monomethyl group to arginine.1 Type 1 PRMT, Coactivator-associated arginine methyltransferase 1 (CARM1), also known as PRMT4, methylates Histone 3 at R2, R17 and R26. CARM1 methylation of H3R17 activates transcription.2-4 PRMT5, which catalyzes H3R8 and H4R3 symmetric dimethylation leads to repression of gene expression, while PRMT1 asymmetric dimethylation of H4R3 is associated with gene activation.4, 5
AnaSpec, EGT Group’s dimethylated arginines peptide sequences for H3 and H4 are listed in Table 1 and 2, respectively. Where both versions are available for the same sequence, the symmetrical version is listed on top, with the asymmetrical equivalent listed below it. Fmoc symmetrical and asymmetrical dimethylated arginines are listed in Table 3.
- Large collection of histone peptides
- Peptides include sequences that have acetylated, methylated, phosphorylated and citrullinated amino acids
- Peptides manufactured in California, USA
Table 1. A listing of Histone H3 sequences containing symmetrical and
asymmetrical dimethylated arginines.
Product
Size
Catalog
#
[Arg(Me2s)2]-Histone
H3 (1-21)-GGK(Biotin); H3R2(Me2s),
biotin-labeled
A-R(Me2s)-TKQTARKSTGGKAPRKQLA-GGK(Biotin)-NH2
0.25
mg
1
mg
64631-025
64631-1
[Arg(Me2a)2]-Histone
H3 (1-21)-GGK(Biotin), H3R2(Me2a),
biotin-labeled
A-R(Me2a)-TKQTARKSTGGKAPRKQLA-GGK(Biotin)-NH2
0.25
mg
1
mg
64630-025
64630-1
[Arg(Me2s)2,
pThr3]-Histone H3
(1-21)-GGK(Biotin)
A-R(Me2s)-pT-KQTARKSTGGKAPRKQLAGG-K(Biotin)
0.25
mg
1
mg
65271-025
65271-1
[Arg(Me2a)2,
pThr3]-Histone H3
(1-21)-GGK(Biotin)
A-R(Me2a)-pT-KQTARKSTGGKAPRKQLAGG-K(Biotin)
0.25
mg
1
mg
65266-025
65266-1
[Arg(Me2s)2,
Lys(Ac)4]-Histone H3
(1-21)-GGK(Biotin)
A-R(Me2s)-T-K(Ac)-QTARKSTGGKAPRKQLAGG-K(Biotin)
0.25
mg
1
mg
65267-025
65267-1
[Arg(Me2a)2,
Lys(Ac)4]-Histone H3 (1-21)-GGK(Biotin)
A-R(Me2a)-T-K(Ac)-QTARKSTGGKAPRKQLAGG-K(Biotin)
0.25
mg
1
mg
65262-025
65262-1
[Arg(Me2s)2,
Lys(Me2)4]-Histone H3
(1-21)-GGK(Biotin)
A-R(Me2s)-T-K(Me2)-QTARKSTGGKAPRKQLAGG-K(Biotin)
0.25
mg
1
mg
65269-025
65269-1
[Arg(Me2a)2,
Lys(Me2)4]-Histone H3 (1-21)-GGK(Biotin)
A-R(Me2a)-T-K(Me2)-QTARKSTGGKAPRKQLAGG-K(Biotin)
0.25
mg
1
mg
65264-025
65264-1
[Arg(Me2s)2,
Lys(Me3)4]-Histone H3
(1-21)-GGK(Biotin)
A-R(Me2s)-T-K(Me3)-QTARKSTGGKAPRKQLAGG-K(Biotin)
0.25
mg
1
mg
65270-025
65270-1
[Arg(Me2a)2,
Lys(Me3)4]-Histone H3
(1-21)-GGK(Biotin)
A-R(Me2a)-T-K(Me3)-QTARKSTGGKAPRKQLAGG-K(Biotin)
0.25
mg
1
mg
65265-025
65265-1
[Arg(Me2s)8]-Histone
H3 (1-21)-K(biotin), H3R8(Me2s)
ARTKQTA-R(Me2s)-KSTGGKAPRKQLA-K(Biotin)
0.25
mg
65351-025
65351-1
[Arg(Me2a)8]-Histone
H3(1-21)-K(Biotin), H3R8(Me2a)
ARTKQTA-R(Me2a)-KSTGGKAPRKQLA-K(Biotin)-NH2
0.25
mg
1
mg
64972-025
64972-1
[Arg(Me2s)17]-Histone
H3 (1-21)-GGK(Biotin);
H3R17(Me2s)
ARTKQTARKSTGGKAP-R(Me2s)-KQLA-GGK(Biotin)-NH2
0.25
mg
1
mg
64633-025
64633-1
[Arg(Me2a)17]-Histone
H3 (1-21)-GGK(Biotin); H3R17(Me2a)
ARTKQTARKSTGGKAP-R(Me2a)-KQLA-GGK(Biotin)-NH2
0.25
mg
1
mg
64634-025
64634-1
[Arg(Me2a)26]-Histone
H3 (15-36)-GGK(Biotin)
APRKQLATKAA-R(Me2a)-KSAPATGGVKGG-K(Biotin)
0.25
mg
1
mg
65261-025
65261-1
Table 2. A listing of Histone H4 sequences containing symmetrical and
asymmetrical dimethylated arginines.
Product
Size
Catalog
#
[Arg(Me2a)3]-Histone
H4(1-21)-GGK(Biotin), H4R3(Me2a)
Ac-SG-R(Me2a)-GKGGKGLGKGGAKRHRKVGG-K(Biotin)
0.25
mg
1
mg
64976-025
64976-1
[Arg(Me2s)3]-Histone
H4 (1-23)-GGK(Biotin)
SG-R(Me2s)-GKGGKGLGKGGAKRHRKVLRGG-K(Biotin)
0.25
mg
1
mg
65276-025
65276-1
[Arg(Me2a)3]-Histone
H4 (1-23)-GGK(Biotin)
SG-R(Me2a)-GKGGKGLGKGGAKRHRKVLRGG-K(Biotin)
0.25
mg
1
mg
65278-025
65278-1
Table 3. A listing of Fmoc symmetrical and asymmetrical dimethylated
arginines.
Product
Size
Catalog
#
Fmoc-Arg(Me)2-OH
(symmetrical)
Fmoc-Nω,ω-dimethyl-L-arginine (symmetrical)
250
mg
1
g
26217-F025
26217-F1
Fmoc-Arg(Me)2-OH
(asymmetrical)
Fmoc-Nω,ω-dimethyl-L-arginine
(asymmetrical)
250
mg
1
g
26218-F025
26218-F1
To view the entire listing of Histone peptides, please click: H1,
H2A, H2B;H3 (1-21) ; H3
(21-44);H4.
The catalog numbers of the PDF listing are hyperlinked to the online product page.
AnaSpec,EGT Group is the sole provider of the industry’s long wavelength
HDAC assay kit, the SensoLyte 520 HDAC Activity Assay Kit.
We also provide unusual amino acid building blocks such as Lys(Ac),
Lys(Me1-3),Arg(Me2),pSer
and pThrfor histone peptide synthesis.
Reference:
- Bedford MT, et al. Mol Cell 18, 263 (2005).
- Chen, D. et al. Science 284, 2174 (1999).
- Ma, H. et al. Curr Biol 11, 1981 (2001).
- Schurter, BT. et al. Biochem 40, 5747 (2001).
- Pal, S. et al. Mol Cell Biol 24, 9630 (2004).