Anti-His
& Anti-GPF Antibodies
The
introduction of tags, whether short peptides (6X-His, DYKDDDDK,
c-myc) or proteins (green fluorescent protein – GFP,
glutathione-s-transferase – GST) into recombinant proteins aids in
the characterization, localization or purification of the recombinant
expressed protein.1-3
As a consequence, antibodies raised against fusion tags are useful in
biochemical or immunohistochemistry applications.
AnaSpec,
EGT Group is pleased to offer a wide selection of high quality
monoclonal and polyclonal antibodies against the hexa-His and GFP
tags.
• Wide
selection of anti-His and anti-GFP antibodies
• Polyclonal and
monoclonal antibodies
• Fluorophore or biotin-labeled His tag
antibodies available
Table
1. Anti-His tag antibodies are available as polyclonal or monoclonal
antibodies
Product
Size
Catalog
#
Anti-His-tag
(polyclonal)
100
µg
500
µg
1
mg
29673
29673-500
29673-1000
Anti-His-tag
(monoclonal)
50
µg
61250
Table
2. A listing of Anti-GFP antibodies.
Product
Size
Catalog
#
Anti-GFP
(1A5), monoclonal
100
ug
55887
Anti-GFP
Tag (6A2B3), monoclonal
100
ug
55886
Anti-GFP
Tag
100
ug
500
ug
1
mg
29779
29779-500
29779-1000
Anti-GFP
Tag (CT )
100
ug
53882
Anti-GFP
Tag (NT)
100
ug
54045
Anti-GFP
Tag (IN)
100
ug
53214
Anti-GFP-Tag,
Chicken IgY
100
ug
55423
Figure
1. ICC of HEK293 cells transfected with GFP and probed with
polyclonal Anti-GFP (Cat# 29779). Secondary antibody used was Goat
anti-rabbit, Hilyte Fluor™ 555 labeled (red). Nucleus
counter-stained with DAPI.
Fluorescently
labeled and biotin labeled 6X-His antibodies are also available.
Antibodies are labeled with a selection of dyes, including our
proprietary HiLyte Fluor™ dyes, which span the visible spectrum.
This gives researchers a wide range of wavelength choices for use in
immunohistochemistry or immunofluorescence applications.
Table 3. A listing of biotin and dye-labeled polyclonal Anti-His
antibodies.
Product
Size
Catalog
#
Polyclonal
Anti-His Tag IgG, Biotinylated
100
µg
29673-BIOT
Polyclonal
Anti-His Tag IgG, FITC labeled (Ex/Em=495/520 nm)
100
µg
29673-FITC
Polyclonal
Anti-His Tag IgG, HiLyte Fluor™ 405-labeled (Ex/Em=404/428 nm)
100
µg
29673-H405
Polyclonal
Anti-His Tag IgG, HiLyte Fluor™ 488 labeled (Ex/Em=499/523 nm)
100
µg
29673-H488
Polyclonal
Anti-His Tag IgG, HiLyte Fluor™ 555 labeled (Ex/Em=553/568 nm)
100
µg
29673-H555
Polyclonal
Anti-His Tag IgG, HiLyte Fluor™ 594 labeled (Ex/Em=593/616 nm)
100
µg
29673-H594
Polyclonal
Anti-His Tag IgG, HiLyte Fluor™ 647 labeled (Ex/Em=653/673 nm)
100
µg
29673-H647
Table
4. A listing of biotin and dye-labeled monoclonal Anti-His
antibodies.
Product
Size
Catalog
#
Monoclonal
Anti-His Tag IgG, Biotinylated
100
µg
61250-BIOT
Monoclonal
Anti-His Tag IgG, AMCA labeled (Ex/Em=344/446 nm)
100
µg
61250-AMCA
Monoclonal
Anti-His Tag IgG, FAM labeled (Ex/Em=495/520nm)
100
µg
61250-FAM
Monoclonal
Anti-His Tag IgG, FITC labeled (Ex/Em=495/520nm)
100
µg
61250-FITC
Monoclonal
Anti-His Tag IgG, ROX labeled (Ex/Em=573/602 nm)
100
µg
61250-ROX
Monoclonal
Anti-His Tag IgG, TAMRA labeled (Ex/Em=555/580 nm)
100
µg
61250-TAMR
Monoclonal
Anti-His Tag IgG, HiLyte Fluor™ 405-labeled (Ex/Em=404/428 nm)
100
µg
61250-H405
Monoclonal
Anti-His Tag IgG, HiLyte Fluor™ 488 labeled (Ex/Em=499/523 nm)
100
µg
61250-H488
Monoclonal
Anti-His Tag IgG, HiLyte Fluor™ 555 labeled (Ex/Em=553/568 nm)
100
µg
61250-H555
Monoclonal
anti-His Tag IgG, HiLyte Fluor™ 594-labeled (Ex/Em=593/616 nm)
100
µg
61250-H594
Monoclonal
Anti-His Tag IgG, HiLyte Fluor™ 647 labeled (Ex/Em=653/673 nm)
100
µg
61250-H647
Related
Products
Full
listing of tag antibodies
Speedy
28-Day Polyclonal Antibody Service
70-Day
Custom Polyclonal Antibody Service
Custom
Hybridoma Development and Monoclonal Antibody Production
References:
- Zheng et al. J Biochem 133, 577 (2003).
- Amrein et al. PNAS 92, 1048 (1995).
- Nakajima K. and Yaoita Y. Nucleic Acids Res 25, 2231 (1997).
- Medina et al. Nucleic Acids Res 28, I (2000).