PTM Lysines and Arginines

Proteins undergo post-translational modifications (PTM) as a mechanism to expand the coding capacity of genes.¹ PTMs found in histone “tails” (amino termini) such as acetylation, methylation and phosphorylation affect chromatin structure and exquisitely regulate gene expression.^2-7

The use of synthetic histone peptides containing PTMs has helped unraveled some of the mysteries of PTMs and their interaction with downstream effectors. The use of 15-20 amino acid long histone peptides containing biotin either on the N or C-terminus used in pull-down assays has advanced the understanding of these epigenetic relationships.^8-9

At AnaSpec, EGT Group, we are pleased to be a source of premium quality amino acids, whether Fmoc or Boc protected, for PTM peptide synthesis. In addition to PTM lysines and arginines, Fmoc protected phosphorylated D and L-serines and threonines are also available.

Acetylated Lysines

Product		Size	Catalog #
Fmoc-Lys(Ac)-OH N-α-Fmoc-N-ε-acetyl-L-lysine, CAS# 159766-56-0		1 g 5 g	22502 23356
Boc-Lys(Ac)-OH N-α-t-Boc-N-ε-acetyl-L-lysine, CAS# 6404-26-8		1 g 5 g	23084 23085

Methylated (mono, di & tri) Lysines

Product		Size	Catalog #
Fmoc-Lys(Me, Boc)-OH N-α-Fmoc-N-ε,ε-t-Boc-methyl-L-lysine		0.25 g 1 g 5 g	60327-025 60327-1 60327-5
Fmoc-Lys(Me)2-OH • HCl Fmoc-Lys(Me)2-OH chloride; Fmoc-Lys(Me)2–OH CAS# 252049-10-8		250 mg 1 g	28211 28212
Boc-Lys(Me)2-OH Boc-Nε,ε -dimethyl-L-lysine		250 mg 1 g	60025-025 60025-1
Fmoc-Lys(Me3)-OH chloride CAS# 201004-29-7		250 mg 500 mg 1 g	53099-F025 53099-F05 53099-F1

Methylated (mono & di- symmetrical & asymmetrical) Arginines

Product		Size	Catalog #
Fmoc-Arg(Me)(Pbf)–OH CAS# 135616-49-7		0.25 g 1 g	65281-025 65281-1
Fmoc-Arg(Me)2-OH (asymmetrical) Fmoc-Nω,ω-dimethyl-L-arginine (asymmetrical) CAS# 268564-10-9		250 mg 1 g	26218-F025 26218-F1
Fmoc-Arg(Me)2-OH (symmetrical) Fmoc-Nω,ω-dimethyl-L-arginine (symmetrical)		250 mg 1 g	26217-F025 26217-F1

Deiminated (citrullinated) Arginines

Product		Size	Catalog #
Fmoc-DL-citrulline		1 g 5 g	26216-F1 26216-F5
Boc-DL-citrulline		1 g 5 g	26216-B1 26216-B5
Fmoc-L–citrulline CAS# 133174-15-9		1 g 5 g	21359 21361
Boc-L–citrulline CAS# 45234-13-7		1 g 5 g	26215-B1 26215-B5

Phosphorylated Serines

Product		Size	Catalog #
Fmoc-Ser[PO(OBzl)-OH]-OH N-α-Fmoc-O-benzyl-L–phosphoserine CAS# 158171 - 14 - 3		1 g 5 g 25 g 50 g 100 g 1 cartridge 10 cartridges	20419 20420 20420-25 20420-50 20420-100 29869 23219
Fmoc-D-Ser[PO(OBzl)-OH]-OH N-α-Fmoc-O-benzyl-D-phosphoserine		1 g 5 g	60083-1 60083-5

Phosphorylated Threonines

Product		Size	Catalog #
Fmoc-Thr[PO(OBzl)-OH]-OH N-α-Fmoc-O-benzyl-L-phosphothreonine CAS# 175291-56-2		1 g 5 g 25 g 50 g 100 g	20835 20836 20836-25 20836-50 20836-100
Fmoc-D-Thr[PO(OBzl)-OH]-OH N-α-Fmoc-O-benzyl-D-phosphothreonine		1 g 5 g	60557-1 60557-5

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Reference:

1. Walsh, CT. et al. Angew. Chem. Int. Ed.44, 7342 (2005).
2. Grunstein, M. et al. J. Cell Sci. Suppl.19, 29 (1995).
3. Strahl, BR and CD Allis, Nature403, 41 (2000).
4. Strahl, BD. et al. Proc. Natl. Acad. Sci.96, 14967 (1999).
5. Jenuwein, T. and CD. Allis Science293, 1074 (2001).
6. Sterner, DE. et al. Microbiol. Mol. Biol. Rev. 64, 435 (2000).
7. Lachner, M. et al. J. Cell Sci.116, 2117 (2003).
8. Protocol Online
9. Wysocka, J. Methods40, 339 (2006).