Proteins undergo post-translational modifications (PTM) as a mechanism to expand the coding capacity of genes.1 PTMs found in histone “tails” (amino termini) such as acetylation, methylation and phosphorylation affect chromatin structure and exquisitely regulate gene expression.2-7
The use of synthetic histone peptides containing PTMs has helped unraveled some of the mysteries of PTMs and their interaction with downstream effectors. The use of 15-20 amino acid long histone peptides containing biotin either on the N or C-terminus used in pull-down assays has advanced the understanding of these epigenetic relationships.8-9
At AnaSpec, EGT Group, we are pleased to be a source of premium quality amino acids, whether Fmoc or Boc protected, for PTM peptide synthesis. In addition to PTM lysines and arginines, Fmoc protected phosphorylated D and L-serines and threonines are also available.
Methylated (mono, di & tri) Lysines
Methylated (mono & di- symmetrical & asymmetrical) Arginines
Deiminated (citrullinated) Arginines
Please click here to obtain a custom peptide price quotation.
- Walsh, CT. et al. Angew. Chem. Int. Ed.44, 7342 (2005).
- Grunstein, M. et al. J. Cell Sci. Suppl.19, 29 (1995).
- Strahl, BR and CD Allis, Nature403, 41 (2000).
- Strahl, BD. et al. Proc. Natl. Acad. Sci.96, 14967 (1999).
- Jenuwein, T. and CD. Allis Science293, 1074 (2001).
- Sterner, DE. et al. Microbiol. Mol. Biol. Rev. 64, 435 (2000).
- Lachner, M. et al. J. Cell Sci.116, 2117 (2003).
- Protocol Online
- Wysocka, J. Methods40, 339 (2006).