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Dual-Glo Luciferase Assay System
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Dual-Glo Luciferase Assay System
Dual-Glo Luciferase Assay System
Dual-Glo Luciferase Assay System from Promega
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Citations:
(191)
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Dual-Glo Luciferase Assay System
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Description
A homogeneous reagent system that enables fast, simple quantitation of both firefly and Renilla luciferase luminescence signals from cells that have not been preconditioned or prelysed
Product Specs
Company Info
Citations
Reviews
Product Reviews
Product Specs
Item
Dual-Glo Luciferase Assay System
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
E2980
Reactivity
mammalian
Detection Target
Luciferase
Species
mammalian
Method
Luciferase
Sample Type
Mammalian cell culture media
Quantity
10 x 100ml
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Promega
2800 Woods Hollow Rd.
Madison
,
Wisconsin
United States
Phone
: 800-356-9526
Fax: 800-356-1970
Company Profile
Website:
http://www.promega.com
Citations (191)
(1)
Programmable modulation of ribosomal frameshifting by mRNA targeting CRISPR-Cas12a system
iScience
December 15, 2023
Shih-Hong Huang, Shih-Cheng Chen, Tsu-Ying Wu, Cheng-Yao Chen, Chien-Hung Yu
screen,Amersham, Rabbit Reticulocyte Lysate,
Promega
,Cat#
L4960
Luciferase Assay System
,
Promega
,Cat#
E2940
,,
Experimental models
: Cell lines,
Experimental models
: Cell lines,
Experimental models
: Cell lines ,, HEK293T,
ATCC
,Cat#
(2)
Non-overlapping epitopes on the gHgL-gp42 complex for the rational design of a triple-antibody cocktail against EBV infection
Cell Reports Medicine
November 21, 2023
Junping Hong, Ling Zhong, Liqin Liu, Qian Wu, Wanlin Zhang, Kaiyun Chen, Dongmei Wei, Hui Sun, Xiang Zhou, Xinyu Zhang,
et al.
100000840 IGEPAL CA-720,Sigma Aldrich,Cat # 238589
Dual-Glo luciferase assay system
,
Promega
,Cat#
E2940
Tissue DNA kit,Omega,Cat# D3396-02 EBER detection kit,ZSGB-BIO,Cat# ISH-7001–100 ,, Deposited data,Deposited
(3)
FUS-dependent microRNA deregulations identify TRIB2 as a druggable target for ALS motor neurons
iScience
November 17, 2023
Wan Yun Ho, Li-Ling Chak, Jin-Hui Hor, Fujia Liu, Sandra Diaz-Garcia, Jer-Cherng Chang, Emma Sanford, Maria J. Rodriguez, Durgadevi Alagappan, Su Min Lim,
et al.
construct (sensor, wild type or seed mutant) per well using Lipofectamine 2000 (Thermo Fisher Scientific,
E2940
) following manufacturer’s protocol. The effectiveness of
miRNA
-mediated
knockdown
is assayed with dual
(4)
Tle4 controls both developmental acquisition and early post-natal maturation of corticothalamic projection neuron identity
Cell reports
August 29, 2023
Galazo MJ, Sweetser DA, Macklis JD.
assays
,, ,, LightCycler Fast start DNA Master SYBER Green I,Roche,Cat # 3515885001 Dual-Glo system,
Promega
,Cat#
E2940
,, Deposited data,, ,, N/A,, ,,
Experimental models
: Organisms/
strains
,, ,, Tle4KO
mice
,"
Wheat
(5)
ZIC2 induces pro-tumor macrophage polarization in nasopharyngeal carcinoma by activating the JUNB/MCSF axis
Cell Death & Disease
July 21, 2023
Qian Liu, Ting Yang, Yu Zhang, Ze-Dong Hu, Yan-Min Liu, Yi-Ling Luo, Shang-Xin Liu, Hua Zhang, Qian Zhong
collected and analyzed for luciferase activity using the
Dual-Glo Luciferase Assay System
(
Promega
, #
E2940
, Madison, WI), and
firefly
luciferase activity was normalized to that of TK-Renilla luciferase according
(6)
Encephalitis and poor neuronal death–mediated control of herpes simplex virus in human inherited RIPK3 deficiency
Science Immunology
April 21, 2023
Zhiyong Liu, Eduardo J. Garcia Reino, Oliver Harschnitz, Hongyan Guo, Yi-Hao Chan, Noopur V. Khobrekar, Mary L. Hasek, Kerry Dobbs, Darawan Rinchai, Marie Materna,
et al.
Reagent (
Roche
).
Luciferase
activities were assessed 24 h later, in a dual luciferase
assay
(
Promega
,
E2940
). Firefly luciferase activities were normalized against Renilla luciferase activities.
Mass cytometry
(7)
Disease-related p63 DBD mutations impair DNA binding by distinct mechanisms and varying degree
Cell Death & Disease
April 18, 2023
Christian Osterburg, Marco Ferniani, Dario Antonini, Ann-Sophie Frombach, Ludovica D’Auria, Susanne Osterburg, Rebecca Lotz, Frank Löhr, Sebastian Kehrloesser, Huiqing Zhou,
et al.
and boiled. The
luciferase reporter assay
was performed using the
Dual-Glo luciferase assay system
(#
E2940
,
Promega
) according to the manufacturers’ recommendation.
Luminescence
signals were measured using a
(8)
Circ_0022920 Maintains the Contractile Phenotype of Human Aortic Vascular Smooth Muscle Cells Via Sponging microRNA‐650 and Promoting Transforming Growth Factor Beta Receptor 1 Expression in Angiotensin II‐Induced Models for Aortic Dissection
Journal of the American Heart Association
April 4, 2023
Mao‐Xun Huang, Hu‐Lin Piao, Yong Wang, Zhi‐Cheng Zhu, Ri‐Hao Xu, Tian‐Ce Wang, Dan Li, Ke‐Xiang Liu
firefly
and
renilla
luciferase activities were examined with the
Dual‐Glo® Luciferase Assay System
(
E2940
,
Promega
) following the product manual. The relative
firefly
luciferase activity was normalized to the
(9)
Divalent siRNAs are bioavailable in the lung and efficiently block SARS-CoV-2 infection
Proceedings of the National Academy of Sciences
March 14, 2023
Vignesh N. Hariharan, Minwook Shin, Ching-Wen Chang, Daniel O’Reilly, Annabelle Biscans, Ken Yamada, Zhiru Guo, Mohan Somasundaran, Qi Tang, Kathryn Monopoli,
et al.
quantified using the
Dual-Glo® Luciferase Assay System
according to the manufacturer′s protocol (
Promega
, #
E2940
).
Luminescence
was detected on a Veritas Luminometer (Promega, #998-9100) or a Tecan M1000 (Tecan).
(10)
NCAPD2 promotes breast cancer progression through E2F1 transcriptional regulation of CDK1
Cancer science
March 1, 2023
He J, Gao R, Yang J, Li F, Fu Y, Cui J, Liu X, Huang K, Guo Q, Zhou Z,
et al
.
GL002‐CDK1‐WT as negative control. According to the instructions of
Promega
dual‐luciferase system (Cat. No.
E2940
), Firefly luciferase value and Renilla luciferase signals were determined. Each experimental analysis
(11)
A bio-orthogonal linear ubiquitin probe identifies STAT3 as a direct substrate of OTULIN in glioblastoma
Nucleic acids research
February 22, 2023
Du X, Pang J, Gu B, Si T, Chang Y, Li T, Wu M, Wang Z, Wang Y, Feng J,
et al
.
NE-PER™ Nuclear and Cytoplasmic Extraction Reagents
(
78835
,
Thermo Fisher
),
Dual-Glo® Luciferase Assay System
(
E2940
,
Promega
),
CellTiter-Glo® Luminescent Cell Viability Assay
(E7572, Promega), FITC Annexin V (640905,
(12)
Signaling events induced by lipopolysaccharide-activated Toll in response to bacterial infection in shrimp
Frontiers in Immunology
February 3, 2023
Sheng Wang, Haoyang Li, Qinyao Li, Bin Yin, Sedong Li, Jianguo He, Chaozheng Li
our previous described (24) and measured with
Dual-Glo Luciferase Assay System
kit (
Promega
, cat. No.
E2940
).
Western blotting
was used to examine 50% of
cell lysis
as inputs. 2.9
Immunofluorescence
and
confocal laser scanning microscopy
(13)
ERCC6L facilitates the progression of laryngeal squamous cell carcinoma by the binding of FOXM1 and KIF4A
Cell death discovery
February 2, 2023
Cui M, Chang Y, Wang J, Wu J, Li G, Tan J.
KIF14A-WT as negative control. According to the instructions of
Promega
dual-luciferase system (Cat. No.
E2940
, Madison, USA), Firefly luciferase value and Renilla luciferase signals were determined. Chromatin
(14)
Genetic and functional odorant receptor variation in the Homo lineage
iScience
January 20, 2023
Claire A. de March, Hiroaki Matsunami, Masashi Abe, Matthew Cobb, Kara C. Hoover
commercial
assays
,Critical commercial
assays
,,
Dual-Glo Luciferase Assay System
,
Promega
,Catalog#
E2940
,, Deposited data,Deposited data,Deposited data Functional Data (raw),This paper,https://github.com/kchoover14/OldNoseshttps://doi.org/10.6084/m9.figshare.21542943 Protein
(15)
Senescent cells perturb intestinal stem cell differentiation through Ptk7 induced noncanonical Wnt and YAP signaling
Nature communications
January 11, 2023
Yun J, Hansen S, Morris O, Madden DT, Libeu CP, Kumar AJ, Wehrfritz C, Nile AH, Zhang Y, Zhou L,
et al
.
firefly
and Renilla luciferase activity was measured using
Dual-Glo Luciferase Assay system
(
Promega
, #
E2940
) on a
Perkin Elmer
EnVision multilabel reader
. The ratios of
firefly
luminescence
to
Renilla
luminescence
(16)
RBM24 inhibits the translation of SARS-CoV-2 polyproteins by targeting the 5'-untranslated region
Antiviral research
January 1, 2023
Yao Y, Sun H, Chen Y, Tian L, Huang D, Liu C, Zhou Y, Wang Y, Wen Z, Yang B,
et al
.
firefly
luciferase was measured at 48 h post
transfection
(hpt) with a
luciferase reporter assay
system (
E2940
;
Promega
). All the reporter
assays
were repeated at least three times. 4.9
In vitro translation
and
(17)
BCR-ABL1-driven exosome-miR130b-3p-mediated gap-junction Cx43 MSC intercellular communications imply therapies of leukemic subclonal evolution
Theranostics
January 1, 2023
Chai C, Sui K, Tang J, Yu H, Yang C, Zhang H, Li SC, Zhong JF, Wang Z, Zhang X.
activity was measured 48h after
transfection
using the
Dual-Glo® Luciferase Assay System
(
Promega
,
E2940
). All the experiments were repeated three times, in triplicate each time. Chromatin immunoprecipitation
(18)
Human hepatocyte-enriched miRNA-192-3p promotes HBV replication through inhibiting Akt/mTOR signalling by targeting ZNF143 in hepatic cell lines
Emerging Microbes & Infections
December 31, 2022
Fahong Li et al.
previously [18]. Luciferase reporter gene
assay
The Dual-Glo
luciferase reporter assay
system (
Promega
,
E2940
) was used to detect the
firefly
luciferase activity and the internal control Renilla luciferase activity,
(19)
The Highly Potent AhR Agonist Picoberin Modulates Hh-Dependent Osteoblast Differentiation
Journal of Medicinal Chemistry
December 22, 2022
Jana Flegel, Saad Shaaban, Zhi Jun Jia, Britta Schulte, Yilong Lian, Adrian Krzyzanowski, Malte Metz, Tabea Schneidewind, Fabian Wesseler, Anke Flegel,
et al.
legends. Luciferase activities were determined using the
Dual-Glo Luciferase Assay System
(
Promega
, #
E2940
) for THP-1 and Hepa1c1c7 cells according to the manufacturer’s instructions. For
NIH-3T3 cells
,
HepG2 cells
(20)
MicroRNA-dependent suppression of biological pacemaker activity induced by TBX18
Cell Reports Medicine
December 20, 2022
Lizbeth Sanchez, Thassio Mesquita, Rui Zhang, Ke Liao, Russell Rogers, Yen-Nien Lin, Rodrigo Miguel-dos-Santos, Akbarshakh Akhmerov, Liang Li, Asma Nawaz,
et al.
assays
,,
Pierce DAB Substrate Kit
,
Thermo Fisher
,Cat# PI34002 Dual
Luciferase reporter Assay
,
Promega
,Cat#
E2940
Click-iT Plus EdU Alexa 594,
Thermo Fisher
,Cat#
C10639
Click-iT Plus EdU Alexa 488,
Thermo Fisher Scientific
,Cat#
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Reviews
Write a Review
(1)
12/20/2018
Detection of Luciferase from Firefly or Renilla
Hong Sun
We wanted to detect renilla luciferase levels among different treatment conditions and normalized to firefly luciferase as internal control. This kit is easy to use and great for luciferase assays.
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Product Reviews
Dual-Glo™ Luciferase Assay System From Promega
Luciferase assays are widely used to quantify gene expression. Typically, they are used following ...
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Dual-Glo Luciferase Assay System from Promega
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