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DeadEnd Colorimetric TUNEL System
DeadEnd Colorimetric TUNEL System from Promega
Be the first to write a review!
Citations:
(152)
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DeadEnd Colorimetric TUNEL System
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Description
A modified TUNEL Assay designed for simple, rapid detection of apoptotic cells in situ at the single-cell level. Measures nuclear DNA fragmentation
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
DeadEnd Colorimetric TUNEL System
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
G7130
Quantity
40 reactions
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Promega
2800 Woods Hollow Rd.
Madison
,
Wisconsin
United States
Phone
: 800-356-9526
Fax: 800-356-1970
Company Profile
Website:
http://www.promega.com
Citations (152)
(1)
Assessment of Hepatic Lesions After non-Thermal Tumor Ablation by Irreversible Electroporation in a Pig Model
Technology in Cancer Research & Treatment
October 20, 2023
Sung-Min Jeon, Enkhzaya Davaa, Yixin Jiang, Ratchapol Jenjob, Ngoc-Thuan Truong, Kyung-Ju Shin, Seok Jeong, Su-Geun Yang
transferase-mediated
dUTP
digoxigenin nick end
labeling
(
TUNEL
)
staining
was also performed with diaminobenzidine (
G7130
,
Promega
, USA) according to the manufacturer's protocol. To investigate the
dilatation
of hepatic sinusoids
(2)
Pioglitazone protects PC12 cells against oxidative stress injury: An in vitro study of its antiapoptotic effects via the PPARγ pathway
Experimental and Therapeutic Medicine
September 22, 2023
Yali Li, Jun Long, Libo Li, Ziyao Yu, Yanjing Liang, Bin Hou, Li Xiang, Xiaolin Niu
experiments performed with triplicate samples.
TUNEL assay
A
DeadEnd™ Colorimetric TUNEL System
(cat. no.
G7130
;
Promega Corporation
) was used to detect PC12 apoptosis in the control, H2O2, pioglitazone + H2O2, pioglitazone
(3)
Absence of Either Ripk3 or Mlkl Reduces Incidence of Hepatocellular Carcinoma Independent of Liver Fibrosis
Molecular cancer research : MCR
September 1, 2023
Mohammed S, Thadathil N, Ohene-Marfo P, Tran AL, Van Der Veldt M, Georgescu C, Oh S, Nicklas EH, Wang D, Haritha NH,
et al
.
performed using paraffin-embedded liver sections (5 μm) by using the
DeadEnd Colorimetric TUNEL System
(#
G7130
,
Promega
) following the manufacturer's instructions. Picrosirius red
staining
Picrosirius red
staining
(4)
The acid-sensing nociceptor TRPV1 controls breast cancer progression in bone via regulating HGF secretion from sensory neurons
Research square
July 5, 2023
Okui T, Hiasa M, Hata K, Roodman GD, Nakanishi M, Yoneda T.
primary antibodies to CD-31 (
1:50
,
Abcam
, #
ab28364
),
DeadEnd™ Colorimetric TUNEL System
(
Promega
, #
G7360
, Madison, WI),
Ki67
(
1:400
,
Cell Signaling
, #
9129
), PGP9.5 (
1:200
,
Abcam
, #
ab8189
), CGRP (
1:200
Abcam
,
(5)
Vernonia amygdalina Ethanol Extract Protects against Doxorubicin-Induced Cardiotoxicity via TGFβ, Cytochrome c, and Apoptosis
Molecules
May 24, 2023
Rony Abdi Syahputra, Urip Harahap, Yahdiana Harahap, Andayana Puspitasari Gani, Aminah Dalimunthe, Amer Ahmed, Satirah Zainalabidin
section immersed in paraffin was stained using
TUNEL
procedures
and a detection kit (
Promega
, Cat. no.
G7130
; Madison, WI, USA). The slides were rehydrated with multilevel ethanol for three minutes, and then washed
(6)
Obesity impairs cardiolipin-dependent mitophagy and therapeutic intercellular mitochondrial transfer ability of mesenchymal stem cells
Cell death & disease
May 13, 2023
Sagar S, Faizan MI, Chaudhary N, Singh V, Singh P, Gheware A, Sharma K, Azmi I, Singh VP, Kharya G,
et al
.
kit,
Invitrogen
,
L3224
6,Dead cell apoptosis kit,Invitrogen,
V13242
7,
DeadEnd™ Colorimetric TUNEL System
,
Promega
,
G7130
8,ATP assay kit,
Abcam
,
ab83355
9,Mouse IL-13 DuoSet
ELISA
,
R&D Systems
,
DY413
,Chemicals,Chemicals,Chemicals 1,Sodium
(7)
Insulin signaling is critical for sinoatrial node maintenance and function
Experimental & Molecular Medicine
May 1, 2023
Sangmi Ock, Seong Woo Choi, Seung Hee Choi, Hyun Kang, Sung Joon Kim, Wang-Soo Lee, Jaetaek Kim
Zeiss AG, Jena, Germany). Apoptosis was determined using a colorimetric DeadEnd™
TUNEL Assay
Kit (#
G7130
;
Promega
Corp., Madison, WI, USA) following the manufacturer’s instructions. Isolation of SAN pacemaker
(8)
BaoShenTongLuo formula protects against podocyte injury by regulating AMPK-mediated mitochondrial biogenesis in diabetic kidney disease
Chinese medicine
March 26, 2023
Guo Y, Wang M, Liu Y, Pang Y, Tian L, Zhao J, Liu M, Shen C, Meng Y, Wang Y,
et al
.
Transferase-Mediated
dUTP
-biotin Nick end Labelling (
TUNEL
) analysis The
DeadEnd™ Colorimetric TUNEL System
(
G7130
/G7160,
Promega
, WI, USA) was used to identify apoptotic glomerular cells in vivo. Paraffin-embedded
(9)
Bile salt hydrolase in non-enterotoxigenic Bacteroides potentiates colorectal cancer
Nature communications
February 10, 2023
Sun L, Zhang Y, Cai J, Rimal B, Rocha ER, Coleman JP, Zhang C, Nichols RG, Luo Y, Kim B,
et al
.
temperature in order to rehydrate the samples. Using the DeadEndTM Colorimetric
TUNEL
System (
Promega
, #
G7130
),
TUNEL
assays
were carried out in accordance with the kit’s instructions. For
TUNEL
signal intensity
(10)
Hepatic ribosomal protein S6 (Rps6) insufficiency results in failed bile duct development and loss of hepatocyte viability; a ribosomopathy-like phenotype that is partially p53-dependent
PLOS Genetics
January 19, 2023
Sarah A. Comerford, Elizabeth A. Hinnant, Yidong Chen, Robert E. Hammer
apoptosis Liver cell apoptosis was determined using the
DeadEnd Colorimetric TUNEL System
(
Promega
, Catalog #
G7360
) applied to de-paraffinized liver sections as recommended by the manufacturer. For quantitation of the
(11)
Glutamine prevents acute kidney injury by modulating oxidative stress and apoptosis in tubular epithelial cells
JCI insight
November 8, 2022
Thomas K, Zondler L, Ludwig N, Kardell M, Lüneburg C, Henke K, Mersmann S, Margraf A, Spieker T, Tekath T,
et al
.
H&E,
TUNEL
,
and IHC
staining
.
TUNEL
staining
was performed with the
DeadEnd Colorimetric TUNEL system
(
G7130
,
Promega
) strictly according to the manufacturer’s instructions. Apoptotic cells in cortex and medulla
(12)
Roles of FoxM1-driven basal β-cell proliferation in maintenance of β-cell mass and glucose tolerance during adulthood
Journal of diabetes investigation
October 1, 2022
Kohata M, Imai J, Izumi T, Yamamoto J, Kawana Y, Endo A, Sugawara H, Seike J, Kubo H, Komamura H,
et al
.
(
TUNEL
) in situ detection, the specimens were processed using a
DeadEnd™ Colorimetric TUNEL System
(
G7360
;
Promega Corporation
, Madison, WI, USA) to detect DNA fragmentation associated with apoptosis. One percent
(13)
A high-throughput drug discovery pipeline to optimize kidney normothermic machine perfusion
Frontiers in Physiology
September 26, 2022
Smilla Hofmann, Florian Grahammer, Ilka Edenhofer, Victor G. Puelles, Tobias B. Huber, Jan Czogalla
Tubular damage was evaluated by two researchers. For
TUNEL
staining
,
Deadend Colorimetric TUNEL System
(
G7130
,
Promega
) was used according to the manufacturer’s specifications. The
incubation
time of
proteinase K
(14)
Stage-Specific Effect of Inositol Hexaphosphate on Cancer Stem Cell Pool during Growth and Progression of Prostate Tumorigenesis in TRAMP Model
Cancers
August 30, 2022
Raina K, Kandhari K, Jain AK, Ravichandran K, Maroni P, Agarwal C, Agarwal R.
for PCNA (#M0879) was from DakoCytomation (Glostrup, Denmark). Dead End Colorimetric
TUNEL
System (#
G7130
) was purchased from
Promega
(Madison, WI, USA). Antibodies for VEGF (#
ab46154
), GLUT4 (#
ab654
), Sox-2
(15)
Supramolecular Hydrogel Based Post-Surgical Implant System for Hydrophobic Drug Delivery Against Glioma Recurrence
International Journal of Nanomedicine
May 16, 2022
Mrunal Vitthal Wanjale, Vishnu Sunil Jaikumar, K C Sivakumar, Riya Ann Paul, Jackson James, G S Vinod Kumar
Antibody (Apoptosis sampler kit
Cell signaling technologies
#
9664
). DeadEnd
Colorimetry
TUNEL
system (
G7130
) was from
Promega
.
Cell culture
inserts were purchased from
Millipore
(Cat No.
MCSP24H48
) and Hi media
(16)
Effects of Iron Supplementation on Testicular Function and Spermatogenesis of Iron-Deficient Rats
Nutrients
May 14, 2022
Chih-Wei Tsao, Yuan-Ru Liao, Ting-Chia Chang, Yih-Fong Liew, Chin-Yu Liu
dUTP
nick end
labeling
(
TUNEL
)
staining
was performed using the DeadEndTM Colorimetric
TUNEL
System (
G7130
,
Promega
, Madison, WI, USA). In brief, 3-μm paraffin-embedded slides were soaked in 0.85% saline for
(17)
The Inhibitory Effects of Anti-ERC/Mesothelin Antibody 22A31 on Colorectal Adenocarcinoma Cells, within a Mouse Xenograft Model
Cancers (Basel)
April 28, 2022
Gentaro Taniguchi et al.
detection of apoptosis in the
tumor
tissues was performed using the DeadEndTM Colorimetric
TUNEL
System (
G7130
,
Promega
, Madison, WI, USA) following the manufacturer’s protocols. As positive controls of
TUNEL assay
,
(18)
Non-apoptotic activation of Drosophila caspase-2/9 modulates JNK signaling, the tumor microenvironment, and growth of wound-like tumors
Cell Reports
April 19, 2022
Derek Cui Cui Xu, Li Wang, Kenneth M. Yamada, Luis Alberto Baena-Lopez
assays
,Critical commercial
assays
,Critical commercial
assays
DeadEnd™ Colorimetric TUNEL System
,
Promega
,Cat#
G7360
"Click-iT™ EdU Cell Proliferation Kit for Imaging, Alexa Fluor™ 647 dye",
ThermoFisher Scientific
,Cat#
(19)
Inhibition of Integrin αvβ6 Activation of TGF-β Attenuates Tendinopathy
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
April 1, 2022
Wang X, Liu S, Yu T, An S, Deng R, Tan X, Crane J, Zhang W, Pan D, Wan M,
et al
.
protocol (
Promega
, Madison, WI, catalog no.
G3250
, for Figures 2, 3, 4F,P, and 6G;
Promega
, catalog no.
G7360
, for Figure 5F).
Immunohistochemistry
and
immunofluorescence
staining
of
human
and
mouse
sections was
(20)
rAAV-delivered PTEN therapeutics for prostate cancer
Molecular Therapy - Nucleic Acids
March 8, 2022
Jianzhong Ai, Jia Li, Qin Su, Hong Ma, Qiang Wei, Hong Li, Guangping Gao
USA).18
TUNEL assay
PCa
cell apoptosis was assessed using the
DeadEnd Colorimetric TUNEL System
from
Promega
(
G7360
, Madison, WI, USA). Briefly, the slides were deparaffinized in two changes of xylene (5 min each), and
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DeadEnd Colorimetric TUNEL System from Promega
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