Dual-Glo Luciferase Assay System E2940 from Promega

Product Specs
ItemDual-Glo Luciferase Assay System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE2940
Reactivitymammalian
Detection TargetLuciferase
Speciesmammalian
Quantity100ml
MethodLuciferase
Sample TypeMammalian cell culture media

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) ALDOC promotes non-small cell lung cancer through affecting MYC-mediated UBE2N transcription and regulating Wnt/β-catenin pathwayAging (Albany NY)September 30, 2023Bin Shang, Fengjuan Lu, Shujuan Jiang, Mengmeng Xing, Xinyu Mao, Guanghai Yang, Hao ZhangRenilla luciferase signal were measured using the Promega (Madison, WI, USA) dual luciferase system (Cat. E2940). The sequences of both UBE2N-WT and UBE2N-MUT DNA in the luciferase reporter assay were listed in “Supplementary

(2) Tle4 controls both developmental acquisition and early post-natal maturation of corticothalamic projection neuron identityCell reportsAugust 29, 2023Galazo MJ, Sweetser DA, Macklis JD.assays,, ,, LightCycler Fast start DNA Master SYBER Green I,Roche,Cat # 3515885001 Dual-Glo system,Promega,Cat# E2940 ,, Deposited data,, ,, N/A,, ,, Experimental models: Organisms/strains,, ,, Tle4KO mice,"Wheat

(3) Mitochondrial carrier 1 (MTCH1) governs ferroptosis by triggering the FoxO1-GPX4 axis-mediated retrograde signaling in cervical cancer cellsCell death & diseaseAugust 8, 2023Wang X, Ji Y, Qi J, Zhou S, Wan S, Fan C, Gu Z, An P, Luo Y, Luo J.fraction was subsequently assayed for luciferase activity with a Dual-Luciferase Reporter Assay System (E2940, Promega Corporation, USA) following the manufacturers’ protocols. Chromatin immunoprecipitation (ChIP)

(4) SOX9 is a key component of RUNX2-regulated transcriptional circuitry in osteosarcomaCell & BioscienceJuly 25, 2023Young-Im Kim, Yu-Chou Tseng, Gamze Ayaz, Shasha Wang, Hualong Yan, Wendy du Bois, Howard Yang, Tao Zhen, Maxwell P. Lee, Paul Liu, et al.Renilla luciferase activities were measured using Dual-Glo® Luciferase Assay System (Promega, Cat: E2940). Normalized luciferase signal was calculated as firefly luciferase signal versus Renilla luciferase

(5) ZIC2 induces pro-tumor macrophage polarization in nasopharyngeal carcinoma by activating the JUNB/MCSF axisCell Death & DiseaseJuly 21, 2023Qian Liu, Ting Yang, Yu Zhang, Ze-Dong Hu, Yan-Min Liu, Yi-Ling Luo, Shang-Xin Liu, Hua Zhang, Qian Zhongcollected and analyzed for luciferase activity using the Dual-Glo Luciferase Assay System (Promega, #E2940, Madison, WI), and firefly luciferase activity was normalized to that of TK-Renilla luciferase according

(6) Structure Optimization of 12β-O-γ-Glutamyl Oleanolic Acid Derivatives Resulting in Potent FXR Antagonist/Modulator for NASH TherapyPharmaceuticals (Basel)May 17, 2023Hao Ma, Yunyang Bao, Shuaishuai Niu, Shaorong Wang, Yiming Li, Hongwei He, Na Zhang, Weishuo Fangduplicated wells. Antagonism activity test: The firefly-Renilla Dual Fluorescence Assay Kit (Promega, #E2940, Madison, WI, USA) was used to measure the antagonism activity of the OA derivatives. Firstly, the firefly

(7) Encephalitis and poor neuronal death–mediated control of herpes simplex virus in human inherited RIPK3 deficiencyScience ImmunologyApril 21, 2023Zhiyong Liu, Eduardo J. Garcia Reino, Oliver Harschnitz, Hongyan Guo, Yi-Hao Chan, Noopur V. Khobrekar, Mary L. Hasek, Kerry Dobbs, Darawan Rinchai, Marie Materna, et al.Reagent (Roche). Luciferase activities were assessed 24 h later, in a dual luciferase assay (Promega, E2940). Firefly luciferase activities were normalized against Renilla luciferase activities. Mass cytometry

(8) Disease-related p63 DBD mutations impair DNA binding by distinct mechanisms and varying degreeCell Death & DiseaseApril 18, 2023Christian Osterburg, Marco Ferniani, Dario Antonini, Ann-Sophie Frombach, Ludovica D’Auria, Susanne Osterburg, Rebecca Lotz, Frank Löhr, Sebastian Kehrloesser, Huiqing Zhou, et al.and boiled. The luciferase reporter assay was performed using the Dual-Glo luciferase assay system (#E2940, Promega) according to the manufacturers’ recommendation. Luminescence signals were measured using a

(9) Circ_0022920 Maintains the Contractile Phenotype of Human Aortic Vascular Smooth Muscle Cells Via Sponging microRNA‐650 and Promoting Transforming Growth Factor Beta Receptor 1 Expression in Angiotensin II‐Induced Models for Aortic DissectionJournal of the American Heart AssociationApril 4, 2023Mao‐Xun Huang, Hu‐Lin Piao, Yong Wang, Zhi‐Cheng Zhu, Ri‐Hao Xu, Tian‐Ce Wang, Dan Li, Ke‐Xiang Liufirefly and renilla luciferase activities were examined with the Dual‐Glo® Luciferase Assay System (E2940, Promega) following the product manual. The relative firefly luciferase activity was normalized to the

(10) Divalent siRNAs are bioavailable in the lung and efficiently block SARS-CoV-2 infectionProceedings of the National Academy of SciencesMarch 14, 2023Vignesh N. Hariharan, Minwook Shin, Ching-Wen Chang, Daniel O’Reilly, Annabelle Biscans, Ken Yamada, Zhiru Guo, Mohan Somasundaran, Qi Tang, Kathryn Monopoli, et al.quantified using the Dual-Glo® Luciferase Assay System according to the manufacturer′s protocol (Promega, #E2940). Luminescence was detected on a Veritas Luminometer (Promega, #998-9100) or a Tecan M1000 (Tecan).

(11) NCAPD2 promotes breast cancer progression through E2F1 transcriptional regulation of CDK1Cancer scienceMarch 1, 2023He J, Gao R, Yang J, Li F, Fu Y, Cui J, Liu X, Huang K, Guo Q, Zhou Z, et al.GL002‐CDK1‐WT as negative control. According to the instructions of Promega dual‐luciferase system (Cat. No. E2940), Firefly luciferase value and Renilla luciferase signals were determined. Each experimental analysis

(12) A bio-orthogonal linear ubiquitin probe identifies STAT3 as a direct substrate of OTULIN in glioblastomaNucleic acids researchFebruary 22, 2023Du X, Pang J, Gu B, Si T, Chang Y, Li T, Wu M, Wang Z, Wang Y, Feng J, et al.NE-PER™ Nuclear and Cytoplasmic Extraction Reagents (78835, Thermo Fisher), Dual-Glo® Luciferase Assay System (E2940, Promega), CellTiter-Glo® Luminescent Cell Viability Assay (E7572, Promega), FITC Annexin V (640905,

(13) Signaling events induced by lipopolysaccharide-activated Toll in response to bacterial infection in shrimpFrontiers in ImmunologyFebruary 3, 2023Sheng Wang, Haoyang Li, Qinyao Li, Bin Yin, Sedong Li, Jianguo He, Chaozheng Liour previous described (24) and measured with Dual-Glo Luciferase Assay System kit (Promega, cat. No. E2940). Western blotting was used to examine 50% of cell lysis as inputs. 2.9 Immunofluorescence and confocal laser scanning microscopy

(14) ERCC6L facilitates the progression of laryngeal squamous cell carcinoma by the binding of FOXM1 and KIF4ACell death discoveryFebruary 2, 2023Cui M, Chang Y, Wang J, Wu J, Li G, Tan J.KIF14A-WT as negative control. According to the instructions of Promega dual-luciferase system (Cat. No. E2940, Madison, USA), Firefly luciferase value and Renilla luciferase signals were determined. Chromatin

(15) Genetic and functional odorant receptor variation in the Homo lineageiScienceJanuary 20, 2023Claire A. de March, Hiroaki Matsunami, Masashi Abe, Matthew Cobb, Kara C. Hoovercommercial assays,Critical commercial assays ,, Dual-Glo Luciferase Assay System,Promega,Catalog# E2940 ,, Deposited data,Deposited data,Deposited data Functional Data (raw),This paper,https://github.com/kchoover14/OldNoseshttps://doi.org/10.6084/m9.figshare.21542943 Protein

(16) Senescent cells perturb intestinal stem cell differentiation through Ptk7 induced noncanonical Wnt and YAP signalingNature communicationsJanuary 11, 2023Yun J, Hansen S, Morris O, Madden DT, Libeu CP, Kumar AJ, Wehrfritz C, Nile AH, Zhang Y, Zhou L, et al.firefly and Renilla luciferase activity was measured using Dual-Glo Luciferase Assay system (Promega, #E2940) on a Perkin Elmer EnVision multilabel reader. The ratios of firefly luminescence to Renilla luminescence

(17) BCR-ABL1-driven exosome-miR130b-3p-mediated gap-junction Cx43 MSC intercellular communications imply therapies of leukemic subclonal evolutionTheranosticsJanuary 1, 2023Chai C, Sui K, Tang J, Yu H, Yang C, Zhang H, Li SC, Zhong JF, Wang Z, Zhang X.activity was measured 48h after transfection using the Dual-Glo® Luciferase Assay System (Promega, E2940). All the experiments were repeated three times, in triplicate each time. Chromatin immunoprecipitation

(18) RBM24 inhibits the translation of SARS-CoV-2 polyproteins by targeting the 5'-untranslated regionAntiviral researchJanuary 1, 2023Yao Y, Sun H, Chen Y, Tian L, Huang D, Liu C, Zhou Y, Wang Y, Wen Z, Yang B, et al.firefly luciferase was measured at 48 h post transfection (hpt) with a luciferase reporter assay system (E2940; Promega). All the reporter assays were repeated at least three times. 4.9 In vitro translation and

(19) Human hepatocyte-enriched miRNA-192-3p promotes HBV replication through inhibiting Akt/mTOR signalling by targeting ZNF143 in hepatic cell linesEmerging Microbes & InfectionsDecember 31, 2022Fahong Li et al.previously [18]. Luciferase reporter gene assay The Dual-Glo luciferase reporter assay system (Promega, E2940) was used to detect the firefly luciferase activity and the internal control Renilla luciferase activity,

(20) The Highly Potent AhR Agonist Picoberin Modulates Hh-Dependent Osteoblast DifferentiationJournal of Medicinal ChemistryDecember 22, 2022Jana Flegel, Saad Shaaban, Zhi Jun Jia, Britta Schulte, Yilong Lian, Adrian Krzyzanowski, Malte Metz, Tabea Schneidewind, Fabian Wesseler, Anke Flegel, et al.legends. Luciferase activities were determined using the Dual-Glo Luciferase Assay System (Promega, #E2940) for THP-1 and Hepa1c1c7 cells according to the manufacturer’s instructions. For NIH-3T3 cells, HepG2 cells

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Dual-Glo™ Luciferase Assay System From Promega
Luciferase assays are widely used to quantify gene expression. Typically, they are used following ... Read More

Dual-Glo Luciferase Assay System

Dual-Glo Luciferase Assay System from Promega

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Dual-Glo™ Luciferase Assay System From Promega

Dual-Glo Luciferase Assay System from Promega

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