Bright-Glo Luciferase Assay System E2610 from Promega

Product Specs
ItemBright-Glo Luciferase Assay System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE2610
ReactivityMammalian
Detection TargetLuciferase
SpeciesMammalian
Sample TypeMammalian cells
MethodLuciferase
Quantity10ml
SensitivityUp to tenfold more light intensity than other homogeneous luciferase assay reagents results in increased sensitivity.

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Non-neutralizing monoclonal antibody targeting VP2 EF loop of Coxsackievirus A16 can protect mice from lethal attack via Fc-dependent effector mechanismEmerging Microbes & InfectionsDecember 31, 2023Ruixiao Du, Chaoqiang An, Xin Yao, Yiping Wang, Ge Wang, Fan Gao, Lianlian Bian, Yalin Hu, Siyuan Liu, Qiaohui Zhao, et al.in 37°C, 5% CO2 cell culture incubator for 24 h. In the next day, add 100μL of Bright-Glo™ reagent (E2620, Promega) to each well and incubate 2 min, then the luminescence was measured. Homology analysis To

(2) Elevated binding and functional antibody responses to SARS-CoV-2 in infants versus mothersNat CommunAugust 11, 2023Caitlin I. Stoddard, Kevin Sung, Zak A. Yaffe, Haidyn Weight, Guillaume Beaudoin-Bussières, Jared Galloway, Soren Gantt, Judith Adhiambo, Emily R. Begnel, Ednah Ojee, et al.controls. After 55 h, luciferase activity was measured using the Bright-Glo Luciferase Assay System (Promega E2610) on a LUMIstar Omega plate reader (BMG Labtech) equipped with Omega software (v5.50 R4). Fraction infectivity

(3) Plasma Glycomic Markers of Accelerated Biological Aging During Chronic HIV InfectionbioRxivAugust 10, 2023Leila B Giron, Qin Liu, Opeyemi S Adeniji, Xiangfan Yin, Toshitha Kannan, Jianyi Ding, David Y. Lu, Susan Langan, Jinbing Zhang, Joao L. L. C. Azevedo, et al.from all wells and replaced with 100 μl of Bright-Glo luciferase substrate reagent (Promega; catalog #E2610). After 2 minutes, the well contents were mixed and transferred to a clear-bottom black 96-well microplate.

(4) Diminished responses to mRNA-based SARS-CoV-2 vaccines in individuals with rheumatoid arthritis on immune modifying therapiesJCI InsightAugust 8, 2023Samuel D. Klebanoff, Lauren B. Rodda, Chihiro Morishima, Mark H. Wener, Yevgeniy Yuzefpolskiy, Estelle Bettelli, Jane H. Buckner, Cate Speake, Marion Pepper, Daniel J. Campbellsupernatant was pipetted off and replaced with Bright-Glo Luciferase Assay System luciferase (Promega, E2610) for 2 minutes at 25°C in the dark before transferring to black-bottom plates for measuring luminescence

(5) DNA-encoded chemical libraries yield non-covalent and non-peptidic SARS-CoV-2 main protease inhibitorsCommun ChemAugust 4, 2023Ravikumar Jimmidi, Srinivas Chamakuri, Shuo Lu, Melek Nihan Ucisik, Peng-Jen Chen, Kurt M. Bohren, Seyed Arad Moghadasi, Leroy Versteeg, Christina Nnabuife, Jian-Yuan Li, et al.At 48h post transfection, media was removed from the wells and 50 µL of Bright-Glo reagent (Promega #E2620) was added to each well and incubated for 5 m before measuring luminescence using the Biotek Synergy H1

(6) Jointly modeling deep mutational scans identifies shifted mutational effects among SARS-CoV-2 spike homologsbioRxivAugust 2, 2023Hugh K. Haddox, Jared G. Galloway, Bernadeta Dadonaite, Jesse D. Bloom, Frederick A. Matsen IV, William S. DeWittvirus titers were measured 48 hours after infection using Bright-Glo Luciferase Assay System (Promega, E2610). Supplementary Material

(7) Optimized conditions for gene transduction into primary immune cells using viral vectorsScientific ReportsJuly 31, 2023Yeongrin Kim, Da Yeon Lee, Ji U Choi, Jin Song Park, So Myoung Lee, Chung Hyo Kang, Chi Hoon Parktransferred into 96-well plates. Cells were mixed with an equal volume of bright-glo assay solution (Promega, E2620) and incubated at room temperature for 10 min with shaking. Half of the mixture was transferred into

(8) SREBP-1 upregulates lipophagy to maintain cholesterol homeostasis in brain tumor cellsCell reportsJuly 25, 2023Geng F, Zhong Y, Su H, Lefai E, Magaki S, Cloughesy TF, Yong WH, Chakravarti A, Guo D.PowerUp™ SYBR™ Green Master Mix,Applied Biosystems,Cat#A25778 Bright-Glo™ Luciferase Assay System,Promega,Cat#E2610 Renilla-Glo™ Luciferase Assay System,Promega,Cat#E2710 Lenti-X Concentrator,Clontech,Cat#631231 KEY

(9) Mapping the neutralizing specificity of human anti-HIV serum by deep mutational scanningCell Host & MicrobeJuly 12, 2023Caelan E. Radford, Philipp Schommers, Lutz Gieselmann, Katharine H.D. Crawford, Bernadeta Dadonaite, Timothy C. Yu, Adam S. Dingens, Julie Overbaugh, Florian Klein, Jesse D. Bloominfected the cells. 48 hours after infection, we used the Bright-Glo Luciferase Assay System (Promega, E2610) to measure relative light units (RLUs) for each dilution. We estimated the average RLU/μL for each

(10) Adaptation of a transmitted/founder simian-human immunodeficiency virus for enhanced replication in rhesus macaquesPLOS PathogensJuly 3, 2023Anya Bauer, Emily Lindemuth, Francesco Elia Marino, Ryan Krause, Jaimy Joy, Steffen S. Docken, Suvadip Mallick, Kevin McCormick, Clinton Holt, Ivelin Georgiev, et al.virus inoculum diluted in growth medium for 48h. After 48h, cells were lysed with Bright-Glo (Promega #E2620), and RLUs were measured on a Luminoskan Ascent luminometer (Thermo Fisher Scientific #5300330). Entry

(11) SARS-CoV-2 Prevalence and Variant Surveillance among Cats in Pittsburgh, Pennsylvania, USAVirusesJune 30, 2023Santhamani Ramasamy, Abhinay Gontu, Sabarinath Neerukonda, Diana Ruggiero, Becky Morrow, Sheweta Gupta, Saranya Amirthalingam, John M. Hardham, Joshua T. Lizer, Michele Yon, et al.(ThermoScientific, Cat. No. 165306). After 48–72 h, the bright-glo luciferase reagent (Promega, Cat. No. E2620) was added to the wells (100 µL per well), the plates were read using BioTek Cytation 5 Cell Imaging

(12) Broad host tropism of ACE2-using MERS-related coronaviruses and determinants restricting viral recognitionCell DiscoveryJune 15, 2023Chengbao Ma, Chen Liu, Qing Xiong, Mengxue Gu, Lulu Shi, Chunli Wang, Junyu Si, Fei Tong, Peng Liu, Meiling Huang, et al.temperature. The intracellular luciferase activity was measured by Bright-Glo Luciferase Assay Kit (Promega, E2620) and detected with a GloMax 20/20 Luminometer (Promega) at 18 h post-infection. GFP intensity was analyzed

(13) Novel Fas-TNFR chimeras that prevent Fas ligand-mediated kill and signal synergistically to enhance CAR T cell efficacyMolecular Therapy - Nucleic AcidsJune 13, 2023Callum McKenzie, Mohamed El-Kholy, Farhaan Parekh, Mathew Robson, Katarina Lamb, Christopher Allen, James Sillibourne, Shaun Cordoba, Simon Thomas, Martin Puleovernight, at which point cells were analyzed with the Bright-Glo Luciferase Assay System (Promega, E2610) according to the manufacturer’s instructions, and then luminescence measured on a Varioskan LUX microplate

(14) Pseudoknot-targeting Cas13b combats SARS-CoV-2 infection by suppressing viral replicationMolecular therapy : the journal of the American Society of Gene TherapyJune 7, 2023Yu D, Han HJ, Yu J, Kim J, Lee GH, Yang JH, Song BM, Tark D, Choi BS, Kang SM, et al.with 100 μL of SARS-CoV-2 pseudovirus. After 24 h, the cells were treated with Bright-Glo Reagent (E2610; Promega) equal to the volume of culture medium in the well. The luminescence was measured with a luminometer

(15) Tumor cell-intrinsic and tumor microenvironmental conditions co-determine signaling by the glycoimmune checkpoint receptor Siglec-7Cellular and Molecular Life SciencesJune 1, 2023Eline J. H. van Houtum, Esther D. Kers-Rebel, Maaike W. Looman, Erik Hooijberg, Christian Büll, Daniel Granado, Lenneke A. M. Cornelissen, Gosse J. Ademaassays described, luminescence was assessed using the Bright-Glo™ Luciferase Assay System (Promega, E2620) according to the manufacturer’s protocol using a Victor3 multilabel plate reader (PerkinElmer). Background

(16) GDNF family receptor alpha-like antagonist antibody alleviates chemotherapy-induced cachexia in melanoma-bearing miceJournal of cachexia, sarcopenia and muscleJune 1, 2023Lee BY, Jeong J, Jung I, Cho H, Jung D, Shin J, Park JK, Park E, Noh S, Shin S, et al.recombinant human GDF15 (10 nM) was added and incubated for 4 h at 37°C. Bright‐Glo Luciferase Assay System (E2620; Promega) was used to activate the luciferase, and the luminescent signal was measured using an EnVision

(17) Structural conservation of Lassa virus glycoproteins and recognition by neutralizing antibodiesCell ReportsMay 30, 2023Hailee R. Perrett, Philip J.M. Brouwer, Jonathan Hurtado, Maddy L. Newby, Lin Liu, Helena Müller-Kräuter, Sarah Müller Aguirre, Judith A. Burger, Joey H. Bouhuijs, Grace Gibson, et al.Assays,Critical Commercial Assays,Critical Commercial Assays ,, Bright-Glo Luciferase Assay System,Promega,Cat# E2620 ,, Deposited Data,Deposited Data,Deposited Data ,, 3D map of LIV GPC-I53-50A (strain Josiah),This

(18) Liver-expressed antimicrobial peptide 2 elevation contributes to age-associated cognitive declineJCI insightMay 22, 2023Tian J, Guo L, Wang T, Jia K, Swerdlow RH, Zigman JM, Du H.expression of luciferase. Treated cells were incubated with Bright-Glo Luciferase Assay solution (Promega, E2610), and the signal was detected using a Biotek Neo2 microplate reader. Cell membrane isolation and membrane

(19) Primate TRIM34 is a broadly-acting, TRIM5-dependent lentiviral restriction factorbioRxivMay 7, 2023Joy Twentyman, Anthony Khalifeh, Abby L. Felton, Michael Emerman, Molly OhAinleAnalyzer (BD Biosciences, San Jose, CA) or Bright-Glo luciferase assay reagent (Promega, Madison, WI #E2620) using a LUMIstar Omega luminometer (BMG Labtech, Ortenberg, Germany). N-MLV Restriction assay HeLa cells

(20) Single-shot AAV-vectored vaccine against SARS-CoV-2 with fast and long-lasting immunityActa pharmaceutica Sinica. BMay 1, 2023Wu F, Luo S, Zhang Y, Ou Y, Wang H, Guo Z, He C, Bai S, He P, Jiang M, et al.at 37 °C in 5% CO2 air environment. Then, 100 μL of Bright Glo luciferase substrate (Promega, USA, #E2620) was added to each well. Two min after incubation at room temperature, 150 μL of lysates was transferred

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Bright-Glo Luciferase Assay System

Bright-Glo Luciferase Assay System from Promega

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Promega

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Bright-Glo™ Luciferase Assay From Promega

Bright-Glo™ Luciferase Assay System From Promega

Bright-Glo Luciferase Assay System from Promega

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