Bright-Glo Luciferase Assay System from Promega

Bright-Glo Luciferase Assay System from Promega

Description

High-throughput quantitation of firefly (Photinus pyralis) luciferase expression in mammalian cells requires highly sensitive reagents that can adapt to continuous-process robotic systems. Bright-Glo Luciferase Assay System is designed specifically to meet the needs of continuous-process systems by providing robust, homogeneous assay chemistry that achieves high assay sensitivity and approximately 30-minute signal half-life without prior sample processing. These attributes also benefit scientists who are using fewer samples but still require high sensitivity and ease of use.

Specifications

  • Detailed Specifications
  • ItemBright-Glo Luciferase Assay System
  • CompanyPromega
  • Price Supplier PageView Company Product Page
  • Catalog NumberE2610
  • Quantity10 ml
  • MethodLuciferase
  • ReactivityMammalian
  • Sample TypeMammalian cells
  • SensitivityUp to tenfold more light intensity than other homogeneous luciferase assay reagents results in increased sensitivity.
  • Detection TargetLuciferase
  • SpeciesMammalian

References

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  • Sevetson, B., Taylor, S. and Pan, Y. (2004) Cbfa1/RUNX2 directs specific expression of the sclerosteosis gene (SOST). J. Biol. Chem. 279(14) , 13849-58
  • Volz, A., Ehlers, A., Younger, R., Forbes, S., Trowsdale, J., Schnorr, D., Beck, S. and Ziegler, A. (2003) Complex transcription and splicing of odorant receptor genes. J. Biol. Chem. 278 (22) , 19691-19701 .
  • Altmann, S.M., Mellon, M.T., Distel, D.L. and Kim, C.H. (2003) Molecular and functional analysis of an interferon gene from the zebrafish, Danio rerio. J. Virol. 77 , 1992-2002 .
  • Chanda, S.K., White, S., Orth, A.P., Reisdorph, R., Miraglia, L., Thomas, R.S., DeJesus, P., Mason, D.E., Huang, Q., Vega, R., Yu, D., Nelson, C.G., Smith, B.M., Terry, R., Linford, A.S., Yu, Y., Chirn, G., Song, C., Labow, M.A., Cohen, D., King, F.J., Peters, E.C., Schultz, P.G., Vogt, P.K., Hogenesch, J.B., and Caldwell, J.S. (2003) Genome-scale functional profiling of the mammalian AP-1 signaling pathway. Proc. Natl. Acad. Sci. USA 100 , 12153-12158 .
  • Rapisarda, A., Uranchimeg, B., Scudiero, D.A., Selby, M., Sausville, E.A., Shoemaker, R.H. and Melillo, G. (2002) Identification of small molecule inhibitors of hypoxia-inducible factor 1 transcriptional activation pathway. Cancer Res. 62(15) , 4316-4324 .
  • Gomez-Escobar, N., Gregory, W.F. and Maizels, R.M. (2000) Identification of tgh-2, a filarial nematode homolog of Caenorhabditis elegans daf-7 and human transforming growth factor B, expressed in microfilarial and adult stages of Brugia malayi. Infect. Immun. 68 (11) , 6402-6410 .
  • Promega
    Promega
    2800 Woods Hollow Rd.
    Madison, Wisconsin
    United States
    Phone: 800-356-9526
    1-800-356-9526
    800-356-1970