B-Raf Kinase Cascade Assay Kit from EMD Millipore

Description

Product Details
Company Info

Detailed Specifications
ItemB-Raf Kinase Cascade Assay Kit
CompanyEMD Millipore
PriceSupplier Page View Supplier Product Page
Catalog Number17-358
Quantity1 kit
ApplicationsIP-Kinase Assay;Kinase Assay
DetectionInquire
FormatRoutinely evalauted by Kinase Assay
MethodRadiometric (32P)
TargetB-Raf
Original Item NameB-Raf Kinase Cascade Assay Kit
Accession NumberNM_004333.3
Source/Expression SystemMolecular biology test kits or supplies

Product Description

B-Raf Kinase Cascade Assay Kit The assay kit is designed to measure B-Raf dependent phosphotransferase activity in a kinase cascade reaction using recombinant MEK1, unactive as a B-Raf substrate. Recombinant MAPK2/Erk2, unactive is phosphorylated and activated by the activated MEK1 leading to phosphorylation of a MAP Kinase substrate, myelin basic protein (MBP). [γ-32P]ATP is the final phosphate donor.

Specifications/Features

B-Raf (δ1-415), active (Cat.# 14-530);MEK1, unactive, rabbit (Cat.# 14-205);MAP Kinase 2/Erk2, unactive (Cat.# 14-198);Assay Dilution Buffer I (ADBI) (Cat.# 20-108);Magnesium/ATP Cocktail (Cat.# 20-113);P81 Phosphocellulose Squares (Cat.# 20-134);Myelin Basic Protein