APC Annexin V Apoptosis Detection Kit with 7-AAD 640930 from BioLegend

Product Specs
ItemAPC Annexin V Apoptosis Detection Kit with 7-AAD
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number640930
Quantity100 tests
ApplicationsFlow Cytometry (FC)
ReactivityHuman, Mouse, Rat
Antibody TypePrimary
NCBI Full Gene Nameannexin A5
NCBI Gene AliasesANX5, CPB-I, ENX2, HEL-S-7, PP4, RPRGL3, VAC-alph
Detection TargetAnnexin V
Conjugate/Tag/LabelAPC

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Company Profile

Website: biolegend.com

(1) PD-L1 Is Involved in the Development of Non-Hodgkin’s Lymphoma by Mediating Circulating Lymphocyte ApoptosisVaccines (Basel)September 11, 2023Manal Mohamed Saberidentified by flow cytometry analyses using an APC Annexin-V apoptosis detection kit (BioLegend, catalog no. 640930). First, the cells were stained for monoclonal antibodies. A cold staining buffer (BioLegend, catalog

(2) Porphyromonas gingivalis Peptidyl Arginine Deiminase (PPAD) in the Context of the Feed-Forward Loop of Inflammation in PeriodontitisInt J Mol SciAugust 18, 2023Zsombor Prucsi, Agnieszka Zimny, Alicja Płonczyńska, Natalia Zubrzycka, Jan Potempa, Maja SochalskaFlow Cytometry The percentage of viable cells was measured by staining with Annexin-V-APC at 1:400 (BioLegend, #640930, San Diego, CA, USA) or Annexin-V eFluor450 diluted at 1:200 (Invitrogen, #88-8007-74, Waltham, MA,

(3) SMAD2 inhibits pyroptosis of fibroblast-like synoviocytes and secretion of inflammatory factors via the TGF-β pathway in rheumatoid arthritisArthritis research & therapyAugust 9, 2023Mao X, Wu W, Nan Y, Sun W, Wang Y.visualized on a Western blotting detection system (Bio-Rad, USA). Flow cytometry The APC Annexin V kit (#640930, Biolegend) was used to detect cell death according to the manufacturer's instructions. Cells were harvested

(4) Impaired regulatory function of granzyme B-producing B cells against T cell inflammatory responses in lupus miceLupus Science & MedicineJuly 27, 2023Jimeng Xue, Liling Xu, Hua Zhong, Mingxin Bai, Xin Li, Ranran Yao, Ziye Wang, Zhen Zhao, Hongchao Li, Huaqun Zhu, et al.IL-17A (Cat# 506904), APC Annexin V Apoptosis Detection Kit with 7-aminoactinomycin D (7-AAD) (Cat# 640930) were purchased from BioLegend (San Diego, California, USA). Phorbol 12-myristate 13-acetate (PMA),

(5) From a drug repositioning to a structure-based drug design approach to tackle acute lymphoblastic leukemiaNature CommunicationsMay 29, 2023Magali Saez-Ayala, Laurent Hoffer, Sébastien Abel, Khaoula Ben Yaala, Benoit Sicard, Guillaume P. Andrieu, Mehdi Latiri, Emma K. Davison, Marco A. Ciufolini, Paul Brémond, et al.cell death was assayed using Annexin V-APC and 7AAD following manufacturer’s instructions (Biolegend, 640930). For γH2A.X measurements, cells were harvested after 24 h, fixed in 4% paraformaldehyde and permeabilized

(6) Collateral activity of the CRISPR/RfxCas13d system in human cellsCommunications biologyMarch 28, 2023Shi P, Murphy MR, Aparicio AO, Kesner JS, Fang Z, Chen Z, Trehan A, Guo Y, Wu X.assays Annexin V and DAPI staining assays were performed using the apoptosis detection Kit (BioLegend, 640930) according to the manufacturer’s protocol. Briefly, 48 h after transfection, cells were washed twice

(7) Salivary gland LAMP3 mRNA expression is a possible predictive marker in the response to hydroxychloroquine in Sjögren's diseasePloS oneJanuary 1, 2023Nakamura H, Tanaka T, Ji Y, Zheng C, Afione SA, Warner BM, Oliveira FR, Motta ACF, Rocha EM, Noguchi M, et al.apoptosis in vitro, A253 cells were stained using the APC Annexin V Apoptosis Detection Kit with 7-AAD (#640930, BioLegend, USA), and apoptotic cells were detected using the BD Accuri Flow Cytometer (BD Biosciences,

(8) BCR-ABL1-driven exosome-miR130b-3p-mediated gap-junction Cx43 MSC intercellular communications imply therapies of leukemic subclonal evolutionTheranosticsJanuary 1, 2023Chai C, Sui K, Tang J, Yu H, Yang C, Zhang H, Li SC, Zhong JF, Wang Z, Zhang X.instructions. The vitality of CIK cells was analyzed using an Annexin V-APC Apoptosis Detection Kit (BioLegend, 640930), according to the manufacturer's instructions. RNA isolation and library preparation for bulk transcriptome profiling

(9) Search for Synergistic Drug Combinations to Treat Chronic Lymphocytic LeukemiaCellsNovember 18, 2022Eleonora Ela Hezkiy, Santosh Kumar, Valid Gahramanov, Julia Yaglom, Arkadi Hesin, Suchita Suryakant Jadhav, Ekaterina Guzev, Shivani Patel, Elena Avinery, Michael A. Firer, et al.Coulter flow cytometer) [17], (iv) flow cytometry analysis of apoptosis using annexin V binding kit (Cat#640930, Biolegend and cat#ab14085, Abcam), and (v) measuring Caspase substrate cleavage of poly(ADP-ribose)

(10) Lamin A/C-dependent chromatin architecture safeguards naïve pluripotency to prevent aberrant cardiovascular cell fate and functionNature communicationsNovember 4, 2022Wang Y, Elsherbiny A, Kessler L, Cordero J, Shi H, Serke H, Lityagina O, Trogisch FA, Mohammadi MM, El-Battrawy I, et al.min at room temperature according to the APC Annexin V Apoptosis Detection Kit with 7-AAD (Biolegend, 640930) instructions. Cells were then resuspended in 400 µl of Annexin V Binding Buffer and subjected to FACS

(11) The neonatal Fc receptor expression during macrophage differentiation is related to autophagyFront ImmunolNovember 1, 2022Juliette Lamamy, Anthony Larue, Julie Mariot, Christine DhommÃ©e, Marie-VÃ©ronique Demattei, Yves Delneste, ValÃ©rie Gouilleux-GruartCell viability/apoptosis was detected using the APC Annexin V Apoptosis Detection Kit with 7-AAD (#640930, Biolegend, USA). Fluorescence was measured with a Cytoflex cytometer (Beckman Coulter). Data were analyzed

(12) Nociceptor neurons affect cancer immunosurveillanceNatureNovember 1, 2022Balood M, Ahmadi M, Eichwald T, Ahmadi A, Majdoubi A, Roversi K, Roversi K, Lucido CT, Restaino AC, Huang S, et al.and collected by centrifugation (5 min at 1,300 rpm), stained using anti-Annexin V, 7-AAD (BioLegend, 640930) and anti-CD8 for 20 min at 4 °C, and immunophenotyped by flow cytometry using a FACSCanto II (Becton

(13) Dysregulation of miR-1-3p: An Early Event in Colitis-Associated DysplasiaInternational Journal of Molecular SciencesOctober 27, 2022Mariana F. Fragoso, Geysson J. Fernandez, Lisa Vanderveer, Harry S. Cooper, Michael Slifker, Margie L. ClapperAnnexinV+/7AAD+ and 7AAD+ only) were analyzed, following staining with APC AnnexinV and 7AAD (Cat #640930, Biolegend, San Diego, CA, USA), according to the manufacturer’s instructions. The cell cycle profile

(14) CD6 deficiency impairs early immune response to bacterial sepsisiScienceOctober 21, 2022Cristina Català et al.paraformaldehyde). For apoptosis analysis APC Annexin V Apoptosis Detection Kit with 7-AAD (Biolegend, 640930) was used following manufacturer’s instructions. Labeled cells were analyzed with a BD FACSCanto II

(15) Ac2-26 attenuates hepatic ischemia-reperfusion injury in mice via regulating IL-22/IL-22R1/STAT3 signalingPeerJSeptember 28, 2022Wanzhen Li et al.(Shanghai, China). APC Annexin V apoptosis detection kit with 7-AAD (Annexin V-APC/7-AAD) assay kit (640930) was purchased from Biolegend (San Diego, California, USA). Anti-TNF- α (K009343M), radioimmunoprecipitation assay

(16) Enforcing GLUT3 expression in CD8+ T cells improves fitness and tumor control by promoting glucose uptake and energy storageFrontiers in ImmunologySeptember 20, 2022Elisabetta Cribioli, Greta Maria Paola Giordano Attianese, Pierpaolo Ginefra, Amandine Signorino-Gelo, Romain Vuillefroy de Silly, Nicola Vannini, Christoph Hess, Melita Irving, George Coukosthe manufacturer’s suggestions. For Annexin V/7-AAD staining the Apoptosis Detection kit was used (640930, Biolegend) according to the manufacturer’s protocol. For cell proliferation, the Cell Trace Violent

(17) Fatty acid metabolism in aggressive B-cell lymphoma is inhibited by tetraspanin CD37Nature communicationsSeptember 13, 2022Peeters R, Cuenca-Escalona J, Zaal EA, Hoekstra AT, Balvert ACG, Vidal-Manrique M, Blomberg N, van Deventer SJ, Stienstra R, Jellusova J, et al.were established by assessing peak intensities. AnnexinV/7-AAD (1:100, BioLegend, San Diego, CA, USA, 640930) stainings were used to determine viability after inhibition using flow cytometry (MACS Quant, Miltenyi

(18) Venetoclax enhances the efficacy of therapeutic antibodies in B-cell malignancies by augmenting tumor cell phagocytosisBlood AdvancesAugust 23, 2022Denis Martin Vogiatzi, Fotini Vogiatzi, Kristina Heymann, Dorothee Winterberg, Dorothee Winterberg, Thies RÃ¶sner, Lennart RÃ¶sner, Michelle Heib, Carina Lynn Gehlert, Carina Lynn Gehlert, et al.isothiocyanate (FITC) (A07768), CD20-FITC (A07772), CD38-FITC (A07778), and Biolegend (Annexin V, #640930). Anti-murine macrophages markers F4-80 and CD11b were obtained from Miltenyi. Xenografts and patient

(19) Stromal remodeling regulates dendritic cell abundance and activity in the tumor microenvironmentCell reportsAugust 16, 2022Papadas A, Deb G, Cicala A, Officer A, Hope C, Pagenkopf A, Flietner E, Morrow ZT, Emmerich P, Wiesner J, et al.manufacturer’s instructions (APC Annexin V Apoptosis Detection Kit with 7-AAD, Biolegend, catalog #640930). Each sample was analyzed by flow cytometry with proper machine settings. Antigen presentation assay MutuDC1940

(20) Mitochondrial fission induces immunoescape in solid tumors through decreasing MHC-I surface expressionNature communicationsJuly 6, 2022Lei X, Lin H, Wang J, Ou Z, Ruan Y, Sadagopan A, Chen W, Xie S, Chen B, Li Q, et al.and fixed in 500 μL of Annexin V binding buffer containing 5 μL Annexin V-APC and 5 μL 7-AAD (Cat. No.640930, BioLegend). Following a 20-min incubation at room temperature in the dark, 20,000 cells were analyzed

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APC Annexin V Apoptosis Detection Kit with 7-AAD from BioLegend

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APC Annexin V Apoptosis Detection Kit with 7-AAD from BioLegend

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