CFSE Cell Division Tracker Kit 423801 from BioLegend

Product Specs
ItemCFSE Cell Division Tracker Kit
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number423801
Quantity1 kit
ApplicationsIntracellular Staining for Flow Cytometry (ICFC), in-vivo Cell Tracking

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Suppression of NK Cell Activation by JAK3 Inhibition: Implication in the Treatment of Autoimmune DiseasesJ Immunol ResDecember 8, 2023Wai Chung Wu, Carol Shiu, Tak Keung Tong, Shui On Leung, Chin Wai Huidays. K562 and MSCs (target cells) were counted and 1 × 106 cells were labeled with 100 µM of CFSE (#423801, BioLegend) for 10 min at 37°C. Labeled target cells were washed and resuspended in complete ATCC-modified

(2) Macrophage infiltration in 3D cancer spheroids to recapitulate the TME and unveil interactions within cancer cells and macrophages to modulate chemotherapeutic drug efficacyBMC CancerDecember 7, 2023Khushwant Singh, Pramod K. Gautam130–123-310) antibodies were used. CalcineAM (Cat. No. 13529), Mitospy (Cat. No. 50–207-9911), CFSE (Cat. No. 423801) were purchased from Biolegend (San Diego, USA) and DAPI Solution (Cat. No. 62247) and Cobalt(II) chloride

(3) Protocol for PPP1R15A-inhibited mouse model establishment with subcutaneous B16F1 tumor and single-cell analysisSTAR ProtocolsDecember 1, 2023Rongjing Wang, Minghui Wang, Siyu Pei, Yuchao Zhang, Shiwei Guo, Wei Guo, Zhenchuan Wu, Hailong Wang, Yizhe Li, Yufei Zhu, et al.incubate the isolated CD8+ T cells with reagents from a CFSE Cell Division Tracker Kit (BioLegend, 423801) according to the manufacturer’s instructions (https://www.biolegend.com/en-us/products/cfse-cell-division-tracker-kit-9396)

(4) Primary explants of the postnatal thymus allow the expansion of clonogenic thymic epithelial cells that constitute thymospheresStem Cell Research & TherapyOctober 31, 2023Juan M. Ocampo-Godinez, Jose L. Gonzalez-Quiroz, Hector Cote-Palafox, Elizabeth George, Jael A. Vergara-Lope Nuñez, Guillermo Villagomez-Olea, Febe C. Vazquez-Vazquez, Edgar O. Lopez-Villegas, Gloria Leon-Avila, Maria L. Dominguez-Lopez, et al.of IL25 (Additional file 1: Fig. S7a-d). The proliferation assay was performed with CFSE BioLegend® (423801) cell division tracker at 5 mM on day 5 when a considerable number of cells emerged from the explants.

(5) Cold-Induced Reprogramming of Subcutaneous White Adipose Tissue Assessed by Single-Cell and Single-Nucleus RNA SequencingResearch (Wash D C)June 28, 2023Qing Liu, Qiaoyun Long, Jiayu Zhao, Wenjie Wu, Zexin Lin, Wei Sun, Ping Gu, Tuo Deng, Kerry Martin Loomes, Donghai Wu, et al.EDTA). Mouse spleen cells were incubated with 5 μM carboxyfluorescein succinimidyl ester (BioLegend, #423801) in PBS for 30 min at 37 °C in water batch and then cocultured with isolated primary adipocytes from

(6) Synthetic miR-21 decoy circularized by tRNA splicing mechanism inhibited tumorigenesis in glioblastoma in vitro and in vivo modelsMolecular Therapy - Nucleic AcidsJune 13, 2023Hadi Bayat, Mohammad Hossein Pourgholami, Saeid Rahmani, Safura Pournajaf, Seyed Javad MowlaC6 cells were labeled with 1 μL/mL carboxyfluorescein diacetate succinimidyl ester (BioLegend; Cat. #423801) according to the manufacturer’s recommendations. The labeled cells were plated at a number of 1.5 ×

(7) Isolation of slow-cycling cancer cells from lung patient-derived xenograft using carboxyfluorescein-succinimidyl ester retention-mediated cell sortingSTAR ProtocolsJune 1, 2023Jaebeom Cho, Hye-Young Min, Ho-Young Lee,Miltenyi Biotec,130-093-237 gentleMACS Dissociator,Miltenyi Biotec,130-093-235 CFSE Cell Division Tracker Kit,BioLegend,423801 ,, Software and algorithms,Software and algorithms,Software and algorithms ,, FACSDiva software,BD

(8) Low responsiveness of peripheral lymphocytes in extraparenchymal neurocysticercosisPLOS Neglected Tropical DiseasesJune 1, 2023Matthew L. Romo, Rocio Osorio, Andrea Toledo, Roger Carrillo-Mezo, Ricardo Valdez, Marta C. Romano, Edda Sciutto, Gladis Fragoso, Agnès Fleuryby staining 10 x106 PBMC with carboxyfluorescein diacetate succinimidyl ester (CFSE, Biolegend Cat. 423801) at 5μM diluted in RPMI-1640 for 5 minutes then washed twice with RPMI-1640 medium supplemented with

(9) Dual inhibition of IDO1/TDO2 enhances anti-tumor immunity in platinum-resistant non-small cell lung cancerCancer & metabolismMay 24, 2023Wu C, Spector SA, Theodoropoulos G, Nguyen DJM, Kim EY, Garcia A, Savaraj N, Lim DC, Paul A, Feun LG, et al.washed and incubated with 1 µm of carboxyfluorescein succinimidyl ester (CFSE) (BioLegend, cat no. 423801) at 37 °C for 7 min then thoroughly washed with staining buffer before in vitro co-culture. The proliferation

(10) Intrauterine desensitization enables long term survival of human oligodendrocyte progenitor cells without immunosuppressioniScienceMay 19, 2023Dou Ye, Suqing Qu, Yinxiang Yang, Zhaoyan Wang, Qian Wang, Weipeng Liu, Fan Zhang, Qian Guan, Xiaohua Wang, Jing Zang, et al.resuscitated and treated with 5-(and 6)-Carboxyfluorescein diacetate succinimidyl ester (CFSE, 0.1 μM, Cat. #423801, Biolegend, San Diego, California, USA) to assess cell division and proliferation, and were set up without

(11) An Ionic Liquid Ablation Agent for Local Ablation and Immune Activation in Pancreatic CancerAdvanced ScienceApril 1, 2023Junming Huang, Meng Wang, Fu Zhang, Shiyi Shao, Zhuo Yao, Xinyu Zhao, Qida Hu, Tingbo Lianganti‐CD11c‐pc5.5 (560584), and anti‐CD206‐AF647 (565250) antibodies were purchased from BD Biosciences. CFSE (423801), anti‐CD25‐AF700 (102024), and anti‐F4/80‐PE‐Cy7 (157308) antibodies were purchased from Biolegend

(12) T-cells engineered with a novel VHH-based chimeric antigen receptor against CD19 exhibit comparable tumoricidal efficacy to their FMC63-based counterpartsFrontiers in ImmunologyFebruary 16, 2023Fatemeh Nasiri, Pooria Safarzadeh Kozani, Fatemeh Rahbarizadehwas analyzed using the carboxyfluorescein succinimidyl ester (CFSE; BioLegend, San Diego, CA; Cat No. 423801) cell staining assay (22). Briefly, 106 - 107 effector T cells were labeled with 1 μL of 5 mM CFSE per

(13) Toxicity Impacts on Human Adipose Mesenchymal Stem/Stromal Cells Acutely Exposed to Aroclor and Non-Aroclor Mixtures of Polychlorinated BiphenylEnvironmental Science & TechnologyJanuary 31, 2023Riley M. Behan-Bush, Jesse N. Liszewski, Michael V. Schrodt, Bhavya Vats, Xueshu Li, Hans-Joachim Lehmler, Aloysius J. Klingelhutz, James A. Ankrumand plated for co-culture. PBMCs were stained with CFSE Cell Division Tracker dye (BioLegend; Cat: #423801) for 15 min. Any unbound CFSE dye was quenched with RPMI (15% FBS). Two-hundred fifty thousand cells

(14) A drug screening to identify novel combinatorial strategies for boosting cancer immunotherapy efficacyJournal of translational medicineJanuary 13, 2023Zhang Z, Wang G, Zhong K, Chen Y, Yang N, Lu Q, Yuan B, Wang Z, Li H, Guo L, et al.drugs plus B7-H3 CAR T cells respectively. B7-H3 CAR T cells were stained with CFSE (Biolegend, Cat# 423801). The HCT116 spheroids were stained with Calcein/PI (Beyotime, Cat# C2015M). Images were captured at

(15) FOXP3 Isoforms Expression in Cervical Cancer: Evidence about the Cancer-Related Properties of FOXP3Δ2Δ7 in KeratinocytesCancers (Basel)January 5, 2023Natalia Garcia-Becerra, Marco Ulises Aguila-Estrada, Luis Arturo Palafox-Mariscal, Georgina Hernandez-Flores, Adriana Aguilar-Lemarroy, Luis Felipe Jave-Suarezcell index change over time. 2.7. Cell Division Tracking CSFE Cell Division Tracker Kit (Cat. No. 423801, BioLegend, San Diego, CA, USA) was used for cell division analysis. CSFE (carboxyfluorescein succinimidyl

(16) Neutrophil-Derived IL-6 Potentially Drives Ferroptosis Resistance in B Cells in Lupus KidneyMediators of inflammationJanuary 1, 2023Wang Z, Shen J, Ye K, Zhao J, Huang S, He S, Qin Y, Meng L, Wang J, Song J.viability dye eFluor 660 (Invitrogen, #65-0864-14), FerroOrange (Dojindo, #F374), CFSE (Biolegend, #423801), IL6-PE (Biolegend, # 504503), B220-FITC (Biolegend, # 103205), Ki67-APC (Biolegend, # 350514), SLC7A11

(17) Cadonilimab, a tetravalent PD-1/CTLA-4 bispecific antibody with trans-binding and enhanced target binding aviditymAbsJanuary 1, 2023Pang X, Huang Z, Zhong T, Zhang P, Wang ZM, Xia M, Li B.(CHO-K1-PD1-CTLA4 cells constructed by Akeso Biopharma) were collected and labeled with CFSE (Biolegend, Cat.: 423801) (2.5 μM). Macrophages and target cells were co-cultured in DMEM-CM with indicated antibodies. DMEM-CM

(18) Single-cell characterization of leukemic and non-leukemic immune repertoires in CD8+ T-cell large granular lymphocytic leukemiaNature CommunicationsDecember 1, 2022Jani Huuhtanen et al.proliferation assay, cells were washed and incubated with CFSE Cell Division tracker kit (Biolegend Cat. 423801) for 20 min at 37 °C protected from light. Fluorescence was quenched by adding RPMI, washed, resuspended

(19) Protocol to drive human monocyte-to-macrophage polarization in vitro using tumor conditioned mediaSTAR ProtocolsDecember 1, 2022Samantha George, Mirella Georgouli, Victoria Sanz-Morenoassays,Critical commercial assays,Critical commercial assays ,, CFSE Cell Division Tracker Kit,BioLegend,423801 CD14+ Human microbeads,Miltenyi Biotec,130-050-201 LS columns,Miltenyi Biotec,130-042-401 "MACS Comp Bead Kit, anti-mouse Igκ

(20) Evaluation of B7-H3 Targeted Immunotherapy in a 3D Organoid Model of CraniopharyngiomaBiomoleculesNovember 24, 2022Tang M, Chen C, Wang G, Wang Y, Zhang Z, Li H, Lu Q, Wang Z, Zhao S, Yang C, et al.antiB7-H3 CAR-T cells were used at a number of 2 × 104/wells after staining with CFSE (Biolegend, Cat# 423801, San Diego, CA, US). For ADC treatment, 2D and 3D cells were treated with antiB7-H3-DM1 at 5 nM. Images

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9/22/2017CFSE Staining Kit for PBMC staining Max HübnerTo assay long-term T-cell proliferation intensity correlating with immunoparalysis before and after surgical intervention we took advantage of this easy-to-handle assay.

CFSE Cell Division Tracker Kit from BioLegend

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CFSE Cell Division Tracker Kit from BioLegend

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