Total Exosome RNA & Protein Isolation Kit 4478545 from Thermo Fisher Scientific

Product Specs
ItemTotal Exosome RNA & Protein Isolation Kit
CompanyThermo Fisher Scientific
Price Pricing Info Supplier Page View Company Product Page
Catalog Number4478545
Brand/TrademarkInvitrogen™
ApplicationsNext-Generation Sequencing, Real-Time Quantitative PCR (qPCR), Western Blot, microRNA Analysis
SourceBodily Fluid
Nucleic Acid TypeExosome RNA
Detection TargetTotal RNA, Protein, RNA & Protein, miRNA
TypeTotal Exosome RNA & Protein Isolation Kit
Sample TypeOrganelle & Vesicle-Related Samples, Exosomes

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Thermo Fisher Scientific

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Waltham, MA 02451

United States

Phone: 800-678-5599

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Website: www.thermofisher.com

(1) Fibroblast-derived extracellular vesicles as trackable efficient transporters of an experimental nanodrug with fibrotic heart and lung targetingTheranosticsJanuary 1, 2024Jorge RuizdelRio, Gabriela Guedes, Danielle Novillo, Elena Lecue, Ana Palanca, Aitziber L. Cortajarena, Ana V. VillarmKate2 100K-EVs (190 μL of 1.9x1014 EVs/mL) was isolated with the isolation kit (Thermo fisher, ref.:4478545) following the specifications for enriching the sample in small RNAs. Final miRNA concentration obtained

(2) Dynamic Interplay in Tumor Ecosystems: Communication between Hepatoma Cells and FibroblastsInternational Journal of Molecular SciencesSeptember 12, 2023Gábor Petővári, Gábor Tóth, Lilla Turiák, Anna L. Kiss, Krisztina Pálóczi, Anna Sebestyén, Adrián Pesti, András Kiss, Kornélia Baghy, Katalin Dezső, et al.Supplementary Figure S5. RNA from EVs was isolated using a total exosome RNA and protein isolation kit (#4478545, Thermo Fisher Scientific Inc.) following the manufacturer’s protocol. The yield and purity of RNA were

(3) Identifying Treatment Resistance Related Pathways by Analyzing Serum Extracellular Vesicles of Patients With Resistant Versus Regressed RetinoblastomaInvest Ophthalmol Vis SciAugust 21, 2023Radhika Manukonda, Saumya Jakati, Jyothi Attem, Dilip K. Mishra, Tirupathi Rao Mocherla, Mamatha M. Reddy, Khushboo Gulati, Krishna Mohan Poluri, Geeta K. Vemuganti, Swathi Kalikiextracellular vesicles (sEVs) were recovered from serum samples using the commercially available Invitrogen Total Exosome RNA & Protein Isolation Kit (Thermo Fisher Scientific, Waltham, MA, USA) based on exosome precipitation. Briefly, according to manufacturer's

(4) Role of miR-15a-5p and miR-199a-3p in the inflammatory pathway regulated by NF-κB in experimental and human atherosclerosisClin Transl MedAugust 21, 2023Paula González-López, Marta Álvarez-Villarreal, Rubén Ruiz-Simón, Andrea R. López-Pastor, Melina Vega de Ceniga, Leticia Esparza, José L. Martín-Ventura, Óscar Escribano, Almudena Gómez-Hernándezthe exosomes from the plasma samples the total exosome RNA and protein isolation kit (from plasma) (4478545, Thermo Fisher Scientific, Waltham, MA, USA), were used following the protocol recommended by the manufacturer.

(5) Potential of miRNAs in Plasma Extracellular Vesicle for the Stratification of Prostate Cancer in a South African PopulationCancers (Basel)August 4, 2023Dada Oluwaseyi Temilola, Martha Wium, Juliano Paccez, Azola Samkele Salukazana, Hasan H. Otu, Giuseppina M. Carbone, Lisa Kaestner, Stefano Cacciatore, Luiz Fernando Zerbiniusing 500 μL each of isolated EVs using the Invitrogen Total Exosome RNA & Protein Isolation Kit (cat. #4478545). Extracted RNA was eluted in 50 μL nuclease-free water and stored at −80 °C. Sample quality control

(6) Sickle Cell Hemoglobin Genotypes Affect Malaria Parasite Growth and Correlate with Exosomal miR-451a and let-7i-5p LevelsInternational Journal of Molecular SciencesApril 19, 2023Keri Oxendine Harp, Alaijah Bashi, Felix Botchway, Daniel Addo-Gyan, Mark Tetteh-Tsifoanya, Amanda Lamptey, Georgina Djameh, Shareen A. Iqbal, Cecilia Lekpor, Saswati Banerjee, et al.1XPBS, followed by RNA isolation from exosomes using the Total Exosome RNA/Protein Isolation kit (Cat# 4478545, Invitrogen, Waltham, MA, USA) in accordance with the manufacturer’s instructions for sections “1-Isolate

(7) Cancer‐associated fibroblasts promote tumor cell growth via miR ‐493‐5p in intrahepatic cholangiocarcinomaCancer ScienceMarch 1, 2023Katsuya Toshida, Shinji Itoh, Noboru Harada, Akinari Morinaga, Kyohei Yugawa, Takahiro Tomiyama, Yukiko Kosai‐Fujimoto, Takahiro Tomino, Takeshi Kurihara, Yoshihiro Nagao, et al.EVs, EVs were pretreated with the Total Exosome RNA & Protein Isolation Kit (Thermo Fisher Scientific, 4478545) and purified according to the manufacturer's instructions. Total RNA isolated from EVs derived from

(8) Post‐prostatic‐massage urine exosomes of men with chronic prostatitis/chronic pelvic pain syndrome carry prostate‐cancer‐typical micro RNA s and activate proto‐oncogenesMolecular OncologyMarch 1, 2023Laura Schneider, Temuujin Dansranjav, Elena Neumann, Hang Yan, Adrian Pilatz, Hans‐Christian Schuppe, Florian Wagenlehner, Undraga Schagdarsurenginobtained as outlined above using the Total Exosome RNA & Protein Isolation Kit (Invitrogen; cat. no. 4478545) according to the manufacturer's protocol. Exosome RNA was eluted in 50 μL of preheated elution solution

(9) Practical tips and new trends in electrochemical biosensing of cancer-related extracellular vesiclesAnalytical and Bioanalytical ChemistryMarch 1, 2023Patrick Severin Sfragano, Serena Pillozzi, Gerolama Condorelli, Ilaria Palchettiand N. Sacchi to isolate RNA from cells and tissues with a single extraction [86]. Similarly, the “Total Exosome RNA & Protein Isolation Kit” by Life Technologies follows three phases based on (i) an organic extraction with acid phenol:chloroform,

(10) Exosomes mediated fibrogenesis in dilated cardiomyopathy through a MicroRNA pathwayiScienceFebruary 17, 2023Xuebin Fu, Rachana Mishra, Ling Chen, Mir Yasir Arfat, Sudhish Sharma, Tami Kingsbury, Muthukumar Gunasekaran, Progyaparamita Saha, Charles Hong, Peixin Yang, et al.heart tissues.70 RNA from Exo samples was isolated using a Total Exosome RNA Isolation Kit (Invitrogen, 4478545). Generally, the cell and Exo samples were dissociated in the QIAzol Lysis Reagent. Chloroform was added

(11) Electrochemical Genosensing of Overexpressed GAPDH Transcripts in Breast Cancer ExosomesAnalytical ChemistryJanuary 31, 2023Arnau Pallares-Rusiñol, Silio Lima Moura, Mercè Martí, Maria Isabel Pividoriextracted using the total exosome RNA and the protein isolation kit (Thermo Fisher Scientific, ref. 4478545) and analyzed with the Agilent RNA 6000 Nano Kit (ref. 5067-1511, Agilent) by Genomics Bioinformatics

(12) Cancer cell-derived exosomal miR-425-3p induces white adipocyte atrophyAdipocyteDecember 31, 2022Anwen Liu, Wenxia Pan, Shutong Zhuang, Yuanzhi Tang, Haitao ZhangTable S1. MiR-425-3p quantification and transfection Total Exosome RNA and Protein Isolation Kit (4478545, Thermo Fisher Scientific, Vilnius, Lithuania) was used to extract and purify total RNA from exosomes.

(13) COVID-19 plasma exosomes promote proinflammatory immune responses in peripheral blood mononuclear cellsScientific reportsDecember 16, 2022Chen L, Chen R, Yao M, Feng Z, Yuan G, Ye F, Nguyen K, Karn J, McComsey GA, McIntyre TM, et al.eliminate backgrounds. Immunoblotting Total exosome proteins were extracted and purified using the Total Exosome RNA & Protein Isolation Kit (ThermoFisher) following the manufacturer’s instructions with a protease inhibitor cocktail (ThermoFisher).

(14) Circ_0004104 participates in the regulation of ox-LDL-induced endothelial cells injury via miR-942-5p/ROCK2 axisBMC Cardiovascular DisordersDecember 2, 2022Yuanyuan Zhang, Shaojun Wang, Sicong Guo, Xinzhong Zhang, Chuan Yang, Guangsheng Su, Jiye Wandissolved with 200 μL PBS, and stored at − 80 °C. Total exosome RNA and protein isolation kit (cat. no. 4478545; Invitrogen) was used to isolate RNA and proteins from exosomes and the protein levels of exosome surface

(15) Induction of m6A methylation in adipocyte exosomal LncRNAs mediates myeloma drug resistanceJournal of Experimental & Clinical Cancer ResearchDecember 1, 2022Zhiming Wang, Jin He, Duc-hiep Bach, Yung-hsing Huang, Zongwei Li, Huan Liu, Pei Lin, Jing Yangwere lysed in radioimmunoprecipitation assay (RIPA) lysis and extraction buffer and isolated using the total exosome RNA & Protein Isolation Kit (Thermo Fisher Scientific). Cell lysates were then subjected to SDS-PAGE, transferred to a polyvinylidene

(16) Extracellular vesicle-derived miRNAs improve stem cell-based therapeutic approaches in muscle wasting conditionsFrontiers in ImmunologyNovember 14, 2022Laura Yedigaryan, Ester Martínez-Sarrà, Giorgia Giacomazzi, Nefele Giarratana, Bernard K. van der Veer, Alessio Rotini, Silvia Querceto, Hanne Grosemans, Álvaro Cortés-Calabuig, Sara Salucci, et al.software (NIH). 2.7 RNA sequencing (RNA-seq) Total RNA and proteins were isolated from EVs using the Total Exosome RNA & Protein Isolation Kit (Invitrogen) and following manufacturers’ instructions. Samples were verified and processed by the Genomics

(17) Peripheral inflammatory markers during an acute bacterial infection in older patients with and without cognitive dysfunction: A case control studyBrain, Behavior, & Immunity - HealthNovember 1, 2022PatrÃcia Regueira, Ana Rita Silva, Ana LuÃsa Cardoso, Ana Maria Cardoso, InÃªs Baldeiras, Isabel Santana, Joaquim Cerejeiraisolated exosomes was performed employing the Total Exosome RNA & Protein Isolation Kit (Invitrogen, #4478545), according to the manufacturer's recommendations. RNA elution was performed in 35 μl of RNAse free

(18) circRAB3IP modulates cell proliferation by reorganizing gene expression and mRNA processing in a paracrine mannerRNANovember 1, 2022Natasa Josipovic, Karoline K. Ebbesen, Anne Zirkel, Adi Danieli-Mackay, Christoph Dieterich, Leo Kurian, Thomas B. Hansen, Argyris Papantonisshort-term at −80°C. For assessment of circRNA enrichment in HUVECs, exosomes were used for RNA extraction (Total Exosome RNA & Protein Isolation Kit, Invitrogen), according to the manufacturer's instructions and qPCR analysis as above. For extracellular vesicle

(19) Small extracellular vesicles from young adipose-derived stem cells prevent frailty, improve health span, and decrease epigenetic age in old miceScience advancesOctober 21, 2022Sanz-Ros J, Romero-García N, Mas-Bargues C, Monleón D, Gordevicius J, Brooke RT, Dromant M, Díaz A, Derevyanko A, Guío-Carrión A, et al.obtained from different sources were stored in PBS at −80°C; for the isolation of RNA, we used the Total Exosome RNA Protein Isolation Kit (Thermo Fisher Scientific), following the manufacturer’s instructions. Small noncoding RNA expression profiling

(20) MircoRNA in Extracellular Vesicles from Patients with Pulmonary Arterial Hypertension Alters Endothelial Angiogenic ResponseInternational Journal of Molecular SciencesOctober 8, 2022Avinash Khandagale, Padraic Corcoran, Maryam Nikpour, Anders Isaksson, Gerhard WikstrÃ¶m, Agneta Siegbahn, Christina Christerssonplasma) using Ambion mirVana™ miRNA Isolation Kit (ThrmoFisher Scientific, Göteborg, Sweden)or Invitrogen Total Exosome RNA & Protein Isolation Kit (ThrmoFisher Scientific, Göteborg, Sweden), following the supplier’s instructions. In each case, the

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4/30/2018Isolating RNA From Exosomes Rachel CrosslandThe Total Exosome RNA & Protein Isolation Kit can be used to isolate Total RNA and protein from exosomes. In our lab, we use the Total Exosome RNA & Protein Isolation Kit to isolate RNA from exosomes ... Read Review

Total Exosome RNA & Protein Isolation Kit from Thermo Fisher Scientific

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Total Exosome RNA & Protein Isolation Kit from Thermo Fisher Scientific

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