Zombie NIR(TM) Fixable Viability Kit 423106 from BioLegend

Product Specs
ItemZombie NIR(TM) Fixable Viability Kit
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number423106
Quantity500 tests
ApplicationsFlow Cytometry (FC)

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) PD-L1-expressing cancer-associated fibroblasts induce tumor immunosuppression and contribute to poor clinical outcome in esophageal cancerCancer immunology, immunotherapy : CIINovember 1, 2023Kawasaki K, Noma K, Kato T, Ohara T, Tanabe S, Takeda Y, Matsumoto H, Nishimura S, Kunitomo T, Akai M, et al.were also used. Dead cells (1:1000 dilution) were stained using a Zombie NIR Fixable Viability Kit (423106, BioLegend). Stained cells were analyzed using flow cytometry (FACSLyric; BD Biosciences, Franklin Lakes,

(2) SETDB1 Methylates MCT1 Promoting Tumor Progression by Enhancing the Lactate ShuttleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)October 1, 2023She X, Wu Q, Rao Z, Song D, Huang C, Feng S, Liu A, Liu L, Wan K, Li X, et al.min. After washing and centrifugation, the samples were stained with Zombie UV Fixable Viability Kit (423106, BioLegend) to exclude dead cells. Then, the samples were stained with anti‐CD45‐PerCP antibody (103129,

(3) Transcriptional regulation by the NSL complex enables diversification of IFT functions in ciliated versus nonciliated cellsSci AdvAugust 25, 2023Tsz Hong Tsang, Meike Wiese, Martin Helmstädter, Thomas Stehle, Janine Seyfferth, Maria Shvedunova, Herbert Holz, Gerd Walz, Asifa Akhtarresuspended in FACS buffer (0.2% FCS and 2 μM EDTA in PBS) supplemented with 1:500 Zombie-NIR (BioLegend, 423105). For DNA, RNA, or protein analysis, the cells were then passed through a 35-μm cell strainer. GFP-positive

(4) A HER2-targeting antibody-MMAE conjugate RC48 sensitizes immunotherapy in HER2-positive colon cancer by triggering the cGAS-STING pathwayCell death & diseaseAugust 24, 2023Wu X, Xu L, Li X, Zhou Y, Han X, Zhang W, Wang W, Guo W, Liu W, Xu Q, et al.expression on the cell membrane. The TILs were stained with the following antibodies: Zombie NIRTM (423105, Biolegend) for dead cell preclusion, CD45-BV510 (103138, Biolegend), CD3-APC (100236, Biolegend), CD4-FITC

(5) Omicron infection following vaccination enhances a broad spectrum of immune responses dependent on infection historyNature communicationsAugust 21, 2023Hornsby H, Nicols AR, Longet S, Liu C, Tomic A, Angyal A, Kronsteiner B, Tyerman JK, Tipton T, Zhang P, et al.re-suspended in the residual volume (~50 μl). Samples were stained with Live/Dead Zombie NIR (Biolegend, 423106) for 10 min at RT, after which and without washing, the surface antibody cocktail diluted in Brilliant Stain Buffer

(6) STING-IRG1 inhibits liver metastasis of colorectal cancer by regulating the polarization of tumor-associated macrophagesiScienceAugust 18, 2023Liu Y, Sun Q, Zhang C, Ding M, Wang C, Zheng Q, Ma Z, Xu H, Zhou G, Wang X, et al.AB_2136290 β-Actin Rabbit mAb,Abclonal,Cat#AC026; RRID: AB_2768234 Zombie NIR™ Fixable Viability Kit,Biolegend,Cat#423105 Brilliant Violet 510™ anti-mouse CD45 Antibody,Biolegend,Cat#103137; RRID: AB_256139 FITC anti-mouse/human CD11b

(7) Development of a Patient-Derived 3D Immuno-Oncology Platform to Potentiate Immunotherapy Responses in Ascites-Derived Circulating Tumor CellsCancers (Basel)August 16, 2023Thomas J. Gerton, Allen Green, Marco Campisi, Minyue Chen, Iliana Gjeci, Navin Mahadevan, Catherine A. A. Lee, Ranjan Mishra, Ha V. Vo, Koji Haratani, et al.again. Afterwards, the samples were incubated in the dark with Zombie NIR viability dye (Biolegend, 423105, Tokyo, Japan) resuspended in 1× PBS for 15 min. The cells were then washed again with 1× PBS supplemented

(8) Lymph node medulla regulates the spatiotemporal unfolding of resident dendritic cell networksImmunityAugust 8, 2023Ugur M, Labios RJ, Fenton C, Knöpper K, Jobin K, Imdahl F, Golda G, Hoh K, Grafen A, Kaisho T, et al.1708890 RNeasy Plus Micro Kit,QIAGEN,Cat# 74034 Zombie NIR™ Fixable Viability Kit,BioLegend,Cat# 423106 Zombie Aqua™ Fixable Viability Kit,BioLegend,Cat# 423102 ,, Deposited data,Deposited data,Deposited

(9) SAP deficiency aggravates periodontitis possibly via C5a-C5aR signaling-mediated defective macrophage phagocytosis of Porphyromonas gingivalisJournal of advanced researchAugust 1, 2023Wang L, Liang D, Huang Y, Chen Y, Yang X, Huang Z, Jiang Y, Su H, Wang L, Pathak JL, et al.times. Cells were counted and analyzed for cell viability using Zombie NIRTM Fixable Viability Kit (423105, Biolegend, Beijing, China). Cells were stained with 5 μg of PE-Cy7 anti-mouse F4/80 (123114, BioLegend,

(10) Preclinical evaluation of PHH-1V vaccine candidate against SARS-CoV-2 in non-human primatesiScienceJuly 21, 2023Antoni Prenafeta, Gregori Bech-Sàbat, Alexandra Moros, Antonio Barreiro, Alex Fernández, Manuel Cañete, Mercè Roca, Luis González-González, Carme Garriga, Joachim Confais, et al.(Omicron),JPT peptide technologies,PM-SARS2-RBDMUT08-1 Zombie NIR fixable viability stain,BioLegend,Cat# 423105 Streptavidin PE-Cy7 conjugate,eBioscience,Cat# 25-4317-82 ,, Critical commercial assays,Critical

(11) Chronic inflammation, neutrophil activity, and autoreactivity splits long COVIDNature communicationsJuly 14, 2023Woodruff MC, Bonham KS, Anam FA, Walker TA, Faliti CE, Ishii Y, Kaminski CY, Ruunstrom MC, Cooper KR, Truong AD, et al.washed in PBS, and resuspended in a PBS dilution of Zombie NIR fixable viability dye (BioLegend, no. 423106). Cells were washed and fixed at 0.8% paraformaldehyde (PFA) for 10 min at 22 °C in the dark before

(12) OX40L-Armed Oncolytic Virus Boosts T-cell Response and Remodels Tumor Microenvironment for Pancreatic Cancer TreatmentTheranosticsJuly 9, 2023Shiyu Liu, Fan Li, Qiongqiong Ma, Mingjuan Du, Haoran Wang, Yiping Zhu, Li Deng, Wenrui Gao, Chunlei Wang, Yanqin Liu, et al.buffer (CS0001, Leagene). The single cell suspension was stained with Zombie NIR™ Fixable Viability Kit (423105, BioLegend) and immune cell markers (Table S1). Flow cytometry analysis was performed on an LSR Fortessa

(13) Evolutionarily conserved amino acids in MHC-II mediate bat influenza A virus entry into human cellsPLOS BiologyJuly 6, 2023Okikiola M. Olajide, Maria Kaukab Osman, Jonathan Robert, Susanne Kessler, Lina Kathrin Toews, Thiprampai Thamamongood, Jacques Neefjes, Antoni G. Wrobel, Martin Schwemmle, Kevin Ciminski, et al.catalog no. 550826, 1:200) for 30 min on ice. Zombie NIR Fixable Viability Kit (BioLegend, catalog no. 423105, 1:1000) was used to assess live versus dead status of cells. After a final wash and centrifugation

(14) Murine Gammaherpesvirus 68 Efficiently Infects Myeloid Cells Resulting In An Atypical, Restricted Form Of InfectionbioRxivJuly 5, 2023Gabrielle Vragel, Brittany D. Gomez, Rachael E. Kostelecky, Kyra S. Noell, Ashley Tseng, Shirli Cohen, Manaal Dalwadi, Eva M. Medina, Elizabeth A. Nail, Andrew Goodspeed, et al.post-infection, stained with the live/dead discrimination dye Zombie Near-IR (Zombie NIR™, BioLegend, Cat. No. 423105), F4/80-AlexaFluor 674 (clone BM8, 1:200 dilution) in the presence of Fc receptor blockade (clone 2.4G2),

(15) Combined GIP receptor and GLP1 receptor agonism attenuates NAFLD in male APOE∗3-Leiden.CETP miceeBioMedicineJuly 1, 2023Zhixiong Ying, Robin van Eenige, Xiaoke Ge, Christy van Marwijk, Joost M. Lambooij, Bruno Guigas, Martin Giera, Jan Freark de Boer, Tamer Coskun, Hongchang Qu, et al.protocol. After washing with PBS, obtained CD45+ leukocytes were pelleted and stained with Zombie NIR (423106, Biolegend) for 20 min at room temperature. After staining, the cells were washed with PBS and fixated

(16) Human neutralizing antibodies to cold linear epitopes and to subdomain 1 of SARS-CoV-2bioRxivJune 29, 2023Filippo Bianchini, Virginia Crivelli, Morgan E. Abernathy, Concetta Guerra, Martin Palus, Jonathan Muri, Harold Marcotte, Antonio Piralla, Mattia Pedotti, Raoul De De Gasparo, et al.(Thermo Fischer Scientific, 47-0037-41), anti-CD8-APC-eFluor 780 (Invitrogen, 47-0086-42), Zombie NIR (BioLegend, 423105), as well as fluorophore-labeled ovalbumin (Ova) and peptides. Live single Zombie-NIR−CD14−CD16−CD3−CD8−CD20+Ova−peptide-PE+peptide-AF647+

(17) Mycobacterium tuberculosis-Specific CD4 T Cells Expressing Transcription Factors T-Bet or RORγT Associate with Bacterial Control in GranulomasmBioJune 27, 2023Nicole L. Grant, Kristen Kelly, Pauline Maiello, Helena Abbott, Shelby O’Connor, Philana Ling Lin, Charles A. Scanga, JoAnne L. Flynnwashes were performed, the cells were stained with Zombie near infrared (NIR) (BioLegend, catalog number 423105) and diluted 1:1000, per the manufacturer’s recommendation, for 10 min at 4°C in the dark. The cells

(18) Combinatorial BCL-2 family expression in Acute Myeloid Leukemia Stem Cells predicts clinical response to Azacitidine/VenetoclaxCancer DiscoveryJune 2, 2023Alexander Waclawiczek, Aino-Maija Leppä, Simon Renders, Karolin Stumpf, Cecilia Reyneri, Barbara Betz, Maike Janssen, Rabia Shahswar, Elisa Donato, Darja Karpova, et al.Members Thawed cells were stained with Zombie NIR Fixable Viability stain in PBS (BioLegend, cat #423105), followed by staining with cell-surface antibodies (see Supplementary Table S6). Stained cells were

(19) HSV-2 triggers upregulation of MALAT1 in CD4+ T cells and promotes HIV latency reversalJournal of Clinical InvestigationJune 1, 2023Carl A. Pierce, Lip Nam Loh, Holly R. Steach, Natalia Cheshenko, Paula Preston-Hurlburt, Fengrui Zhang, Stephanie Stransky, Leah Kravets, Simone Sidoli, William M Philbrick, et al.Flow cytometry. Cells were harvested and washed twice in PBS (21-040-CV, Corning) and stained with Zombie NIR (423106, BioLegend) for 15 minutes at room temperature. Cells were washed 3 times in 200 mL of FACS buffer (PBS

(20) Conserved transcriptional connectivity of regulatory T cells in the tumor microenvironment informs new combination cancer therapy strategiesNature ImmunologyJune 1, 2023Ariella Glasner, Samuel A. Rose, Roshan Sharma, Herman Gudjonson, Tinyi Chu, Jesse A. Green, Sham Rampersaud, Izabella K. Valdez, Emma S. Andretta, Bahawar S. Dhillon, et al.with Ghost Dye Red 780 (Tonbo Bioscience, 13-0865) or Zombie NIR Flexible Viability Kit (BioLegend, 423106) and a mixture of fluorophore-conjugated antibodies for 30 min at 4 °C cells, washed and fixed with

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(1)

11/29/2023Reliable Zombie NIR™ Fixable Viability Kit From BioLegend Varaang BhandulaThe Zombie NIR viability kit works well for identifying dead cells from cell suspensions isolated from mouse lungs, spleen, and lymph nodes. I recommend storing at -20C to ensure the dye works well ... Read Review
(1)

4/24/2021Relatively Good Fixable Live/Dead Dye Hong SunWe are interested in characterizing the immune populations in secondary lymphoid organs and tumors under different knockout condition.
(1)

1/11/2021Using Zombie NIR to Detect Dead Cells Alejandro PradosWe are using the Zombie NIR to characterize the immune cells populations in the mouse intestine during chronic inflammation. This reagent is perfect to clearly separate dead and live cells.
(1)

3/1/2016Cell Viability Kit for FC Maria Carmela SperanzaThis is a good and easy to use cell viability staining kit for flow cytometry.

Zombie NIR(TM) Fixable Viability Kit from BioLegend

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Zombie NIR(TM) Fixable Viability Kit from BioLegend

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