ToxBLAzer DualScreen Kit from Life Technologies

ToxBLAzer DualScreen Kit from Life Technologies

Description

ToxBLAzer; DualScreen Kit provide the key advantage of combining the ratiometric reporter gene readout of beta-lactamase with a cytotoxicity readout in the same assay. Thus, ToxBLAzer; DualScreen yields reporter readouts that normalize cytotoxic cellular responses, which is a necessity in screening applications which down regulate transcription. The ToxBLAzer; substrate contains both the LiveBLAzer;-FRET B/G substrate and a proprietary cytotoxicity indicator. Both compounds readily enter the cell, where endogenous esterases rapidly convert them into fluorescent compounds Esterase activity and an intact cell membrane are required for detection of the indicator. The cytotoxicity indicator's fluorescence is red-shifted to ensure compatibility with the blue and green emissions of the LiveBLAzer;-FRET B/G substrate, allowing detection of both reporter gene activity and cytotoxicity in the same assay sample.Advantages of ToxBLAzer; DualScreen Kits:Multiplexed readout results in rapid identif

Specifications

  • Detailed Specifications
  • ItemToxBLAzer DualScreen Kit
  • CompanyLife Technologies
  • Price Pricing InfoSupplier PageView Company Product Page
  • Catalog NumberK1138
  • Quantity200 µg
  • ApplicationsBeta-Lactamase Activity Assays:Cell Analysis:Cell Viability & Cytotoxicity:Cell-Based β-Lactamase Assays:Cell-Based GPCR Reporter Assays:Cell-Based Nuclear Receptor Reporter Assays:Cell-Based Reporter Assays:Cellular Pathway Analysis Assays:Drug Discovery & Development:Enzyme & Protein Activity Assays:G-Protein Coupled Receptor Biology:Kinase Biology:Kinase Pathway Analysis:Nuclear Receptor Biology:Other Cellular Pathway Assay Products:Pathway Biology:Proteins, Expression, Isolation & Analysis:Reporter Assays:Target & Lead Identification & Validation:Cellular Imaging & Microscopy:Cell Viability, Proliferation & Function
  • DetectionRatiometric blue:green reporter readout reduces false hits by minimizing experimental noise
  • MethodFluorometric
  • Detection TargetCell viability
Life Technologies
Life Technologies
5791 Van Allen Way
Carlsbad, CA 92008
United States
Phone: (760) 603-7200

Citations