CellTiter-Blue Cell Viability Assay G8081 from Promega

Product Specs
ItemCellTiter-Blue Cell Viability Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberG8081
Detection TargetCell viability
Detectionfluorescence
Quantity100ml
MethodFluorometric (Blue)
Sample TypeLive Cells

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Cornification of keratinocytes is associated with differential changes in the catalytic activity and the immunoreactivity of transglutaminase-1Sci RepDecember 6, 2023Marta Surbek, Tessa Van de Steene, Attila Placido Sachslehner, Bahar Golabi, Johannes Griss, Sven Eyckerman, Kris Gevaert, Leopold Eckhartcorresponding inhibitors were added. CellTiter-Blue assay The CellTiter-Blue assay (catalogue number G8080, Promega), which measures the ability of living cells to convert resazurin into fluorescent resorufin,

(2) Clarithromycin overcomes stromal cell-mediated drug resistance against proteasome inhibitors in myeloma cells via autophagy flux blockage leading to high NOXA expressionPLoS OneDecember 1, 2023Shota Moriya, Hiromi Kazama, Hirotsugu Hino, Naoharu Takano, Masaki Hiramoto, Shin Aizawa, Keisuke Miyazawaof cells The number of viable cells was assessed using CellTiter Blue, a cell viability assay kit (#G8081; Promega, Madison, WI), according to the manufacturer’s instructions, as previously described in detail

(3) Lysophosphatidylserine induces necrosis in pressure overloaded male mouse hearts via G protein coupled receptor 34Nature CommunicationsJuly 31, 2023Ryuta Sugihara, Manabu Taneike, Tomokazu Murakawa, Takahito Tamai, Hiromichi Ueda, Rika Kitazume-Taneike, Takafumi Oka, Yasuhiro Akazawa, Hiroki Nishida, Kentaro Mine, et al.Polar Lipid). After designated periods or concentrations of drug exposure, Cell Titer Blue reagent (G8081, Promega) was added into culture medium 90 min before measurement. Cell viabilities were estimated by

(4) Genome-wide CRISPR screens define determinants of epithelial-mesenchymal transition mediated immune evasion by pancreatic cancer cellsScience AdvancesJuly 14, 2023Yuanzhuo Gu, Zhengkui Zhang, Marcel G. M. Camps, Ferry Ossendorp, Ruud H. Wijdeven, Peter ten DijkeAfter removing culture medium, the cell viability was measured by the CellTiter-Blue Viability Assay (G8081, Promega). In all cases, cell viability was calculated relative to the untreated group, and each group

(5) Protocol to generate an in vitro model to study vascular calcification using human endothelial and smooth muscle cellsSTAR ProtocolsJune 9, 2023Elisa Persiani, Elisa Ceccherini, Ilaria Gisone, Antonella Cecchettini, Federico VozziSmooth Muscle Growth Supplement (SMGS),Thermo Fisher Scientific,S00725 CellTiter-Blue® Cell Viability Assay,Promega,G8081 Calcium Colorimetric Assay kit,Merck,MAK022 Endothelial Cell Growth Medium MV2,PromoCell,C-22221 Endothelial

(6) A new MAPK13-guided inhibitor for respiratory inflammation and mucus productionbioRxivJune 1, 2023Shamus P. Keeler, Kangyun Wu, Yong Zhang, Dailing Mao, Ming Li, Courtney A. Iberg, Stephen R. Austin, Samuel A. Glaser, Jennifer Yantis, Stephanie Podgorny, et al.numbers of viable cells were determined using a resazurin-based CellTiter-Blue Cell Viability Assay (#G8080, Promega, Madison, WI). In addition, hTECs were cultured under 3D-Matrigel conditions to permit organoid

(7) Low level of antioxidant capacity biomarkers but not target overexpression predicts vulnerability to ROS-inducing drugsRedox BiologyJune 1, 2023Jana Samarin, Piotr Fabrowski, Roman Kurilov, Hana Nuskova, Johanna Hummel-Eisenbeiss, Hannelore Pink, Nan Li, Vivienn Weru, Hamed Alborzinia, Umut Yildiz, et al.FLUOstar OPTIMA ELISA reader (550/590 nm) about 2 h after CellTiter-Blue staining (Promega, Cat No. G8081). Mean values±standard deviations (SD) were calculated. EC50 were calculated from dose response curves

(8) Lactoferrin/sialic acid prevents adverse effects of intrauterine growth restriction on neurite length: investigations in an in vitro rabbit neurosphere modelFrontiers in Cellular NeuroscienceApril 26, 2023Britta Anna Kühne, Lara Gutierrez-Vázquez, Estela Sánchez Lamelas, Laia Guardia-Escote, Laura Pla, Carla Loreiro, Eduard Gratacós, Marta Barenys, Miriam Illaviability The cell viability was assessed with the CellTiter-Blue® cell viability assay (Promega #G8081). This assay is based on the measurement of mitochondrial reductase activity of living cells by conversion

(9) First-in-class multifunctional TYMS non-classical antifolate inhibitor with potent in vivo activity that prolongs survivalJCI InsightApril 25, 2023Maria V. Guijarro, Patrick C. Kellish, Peter E. Dib, Nicholas G. Paciaroni, Akbar Nawab, Jacob Andring, Lidia Kulemina, Nicholas V. Borrero, Carlos Modenutti, Michael Feely, et al.reduction of MTS using Cell Titer 96 R Aqueous One Solution Cell Proliferation Assay Kit (Promega, catalog G8081), following manufacturer’s recommendations. Chemiluminescence output (integration time 1,000 ms) was

(10) Reduced C9orf72 expression exacerbates polyGR toxicity in patient iPSC-derived motor neurons and a Type I protein arginine methyltransferase inhibitor reduces that toxicityFrontiers in Cellular NeuroscienceApril 17, 2023Therese L. Dane, Anna L. Gill, Fernando G. Vieira, Kyle R. Dentonfor 24 or 48 h prior to assessing viability by CellTiter-Blue (Promega Corporation, Madison, WI, USA, G8081) fluorescence or live-cell imaging. Doses for exogenous application of polyGR15 were chosen based on

(11) Investigating miR-9 as a mediator in laryngeal cancer health disparitiesFrontiers in OncologyApril 4, 2023Christina Gobin, Samuel Inkabi, Chayil C. Lattimore, Tongjun Gu, James N. Menefee, Mayrangela Rodriguez, Heather Kates, Christopher Fields, Tengfei Bian, Natalie Silver, et al.cisplatin and spent media were aspirated from the wells and a 1:7 dilution of the Cell Titer Blue reagent (G8080, Promega) was applied to the cells in the wells. Baseline fluorescence (560/590 nm) was assessed using

(12) Amphiphilic Cyclodextrin Nanoparticles as Delivery System for Idebenone: A Preformulation StudyMoleculesMarch 28, 2023Federica De Gaetano, Angela Scala, Consuelo Celesti, Kim Lambertsen Larsen, Fabio Genovese, Corrado Bongiorno, Loredana Leggio, Nunzio Iraci, Nunzio Iraci, Antonino Mazzaglia, et al.NPs Cell viability was evaluated with the Cell-Titer-Blue Cell Viability Assay (Promega, Madison, WI, USA, G8080) after 72 h. For the cytotoxicity analysis, the cells were pretreated with NPs (conditions 1–4) for

(13) Components of the sympathetic nervous system as targets to modulate inflammation - rheumatoid arthritis synovial fibroblasts as neuron-like cells?Journal of inflammation (London, England)March 14, 2023Cheng X, Lowin T, Honke N, Pongratz G.RASFs, cells were incubated with CellTiter-Blue reagent following the instruction of the manufacturer (G8081, Promega). By determining the reduction from resazurin to resorufin, the cell viability was estimated

(14) Discovery of natural-product-derived sequanamycins as potent oral anti-tuberculosis agentsCellMarch 2, 2023Jidong Zhang, Christine Lair, Christine Roubert, Kwame Amaning, María Belén Barrio, Yannick Benedetti, Zhicheng Cui, Zhongliang Xing, Xiaojun Li, Scott G. Franzblau, et al.commercial assays,Critical commercial assays,Critical commercial assays ,, CellTiter-Blue,Promega,Cat# G8080 Cell Mask Blue,Invitrogen,Cat# H32720 Maxwell RSC Cultured Cells DNA Kit,Promega,Cat#AS1620 Maxwell

(15) Rescue of lysosomal function as therapeutic strategy for SPG15 hereditary spastic paraplegiaBrainMarch 1, 2023Chiara Vantaggiato, Genny Orso, Giulia Guarato, Francesca Brivio, Barbara Napoli, Elena Panzeri, Simona Masotti, Filippo Maria Maria Santorelli, Maria Lamprou, Sentiljana Gumeni, et al.toxicity analysis Compound toxicity was determined by using the CellTiter-Blue Cell Viability Assay (G8080, Promega), according to the manufacturer’s instructions. Briefly, cells were seeded in 96-well plates

(16) Whole-cell energy modeling reveals quantitative changes of predicted energy flows in RAS mutant cancer cell linesiScienceFebruary 17, 2023Thomas Sevrin, Lisa Strasser, Camille Ternet, Philipp Junk, Miriam Caffarini, Stella Prins, Cian D’Arcy, Simona Catozzi, Giorgio Oliviero, Kieran Wynne, et al.C7026 Pierce™ BCA Protein Assay,ThermoFisher Scientific,# 23225 CellTiter-Blue® Cell Viability Assay,Promega,# G8081 CellTiter-Glo® Luminescent Cell Viability Assay,Promega,# G9241 Glucose-Glo™ Assay,romega,# J6021 Lactate-Glo™ Assay

(17) Multilayer In Vitro Human Skin Tissue Platforms for Quantitative Burn Injury InvestigationBioengineering (Basel)February 17, 2023Sean Brocklehurst, Neda Ghousifam, Kameel Zuniga, Danielle Stolley, Marissa Nichole Rylandersample preparation to establish a baseline prior to burn tests using a CellTiter Blue assay (Promega G8081, Promega, Madison, WI, USA) according to the manufacturer’s procedure. 2.2.2. Spatial Distribution

(18) Three-Dimensional Evaluation of the Cytotoxicity and Antibacterial Properties of Alpha Lipoic Acid-Capped Silver Nanoparticle Constructs for Oral ApplicationsNanomaterials (Basel, Switzerland)February 12, 2023Abdelmoneim D, Porter G, Duncan W, Lim K, Easingwood R, Woodfield T, Coates D.were incubated at 37 °C, 5% CO2, for 20 h, then 40 μL of Prestoblue® cell viability regent (Cat. No. G8080; Promega, In Vitro Technologies, Beverly Hills, CA, USA) was added to each well. The well plates were

(19) A Selective SARS-CoV-2 Host-Directed Antiviral Targeting Stress Response to Reactive Oxygen SpeciesACS central scienceJanuary 25, 2023Tang C, Coelho AR, Rebelo M, Kiely-Collins H, Carvalho T, Bernardes GJL.of PL was added to cells for another 24 h incubation. CellTiter Blue viability assay (Promega, Cat#G8080) was used to assess the viability of cells after treatment, according to the manufacturer’s protocol. Viral

(20) p53 engages the cGAS/STING cytosolic DNA sensing pathway for tumor suppressionMolecular cellJanuary 19, 2023Ghosh M, Saha S, Li J, Montrose DC, Martinez LA.Kit,Qiagen,Cat# 27106 Reasy Mini Kit,Qiagen,Cat# 74104 CellTiter-Blue® Cell Viability Assay,Promega,Cat# G8081 AnnexinV-FITC/PI apoptosis detection kit,BiLegend,Cat# 640914 Clarity Max Western ECL Substrate,Bio-Rad,Cat#

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(1)

6/16/2023Excellent and Easy to Use Kit for Monitoring Cell Viability Wei Yang SitThe kit provides a homogeneous, fluorescent method for monitoring cell viability. The assay is based on the ability of living cells to convert a redox dye (resazurin) into a fluorescent end product (... Read Review
(1)

5/1/2015CellTiter-Blue for use in viability assays Robert LangCellTiter-Blue was used to evaluate the viability/proliferation of U2OS cells (ATCC) treated with PLK1 or negative control siRNA to determine the appropriate positive control concentration to measure ... Read Review

CellTiter-Blue™ Cell Viability Assay From Promega
The CellTiter-Blue™ Viability Assay is used to estimate the number of viable cells present in ... Read More

CellTiter-Blue Cell Viability Assay

CellTiter-Blue Cell Viability Assay from Promega

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Promega

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CellTiter-Blue™ Cell Viability Assay From Promega

CellTiter-Blue Cell Viability Assay from Promega

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