Bio-Plex 3D Suspension Array System from Bio-Rad

Product Specs
ItemBio-Plex 3D Suspension Array System
CompanyBio-Rad
Price Get Pricing Get Quote for this Product
Catalog NumberBIOPLEX3D
Brand/TrademarkLSG LSD
QuantityEA
TypeMultiplex Plate Reader
RangeUp to 500 Individual analytes
ApplicationsMultiplex analysis of 500 unique analytes
Plate Formats96- and 384-well plates
Wavelength(s)635 nm (classification), 532 nm (reporter)
Optical SystemLasers/APDs/PMTs

Add to Compare List

Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

Website: Bio-Rad Life Science Research Product Promotions

(1) Convalescent human IgG, but not IgM, from COVID-19 survivors confers dose-dependent protection against SARS-CoV-2 replication and disease in hamstersFrontiers in ImmunologyMarch 21, 2023Hannah A. D. King, Vincent Dussupt, Letzibeth Mendez-Rivera, Bonnie M. Slike, Ursula Tran, Nathan D. Jackson, Erica Barkei, Michelle Zemil, Emily Tourtellott-Fogt, Caitlin H. Kuklis, et al.detection reagent, microspheres were resuspended in 40 μl sheath fluid (Luminex). Data was collected on a Bio-Plex®3D Suspension Array system (Bio-Rad) running xPONENT® v.4.2 (Luminex). Signal to Noise (S/N) ratio were calculated by the dividing

(2) A non-neutralizing glycoprotein B monoclonal antibody protects against herpes simplex virus disease in miceJournal of Clinical InvestigationFebruary 1, 2023Masayuki Kuraoka, Clare Burn Burn Aschner, Ian W. Windsor, Aakash Mahant Mahant Mahant, Scott J. Garforth, Susan Luozheng Kong, Jacqueline M. Achkar, Steven C. Almo, Garnett Kelsoe, Betsy C. HeroldBiotech). After 3 washes, the beads were resuspended in assay buffer, and the plates were read on a Bio-Plex 3D Suspension Array System (Bio-Rad). The following antigens were coupled with carboxylated beads (Luminex Corp.): BSA (Affymetrix),

(3) Infant Antibody Repertoires during the First Two Years of Influenza VaccinationmBioDecember 20, 2022Masayuki Kuraoka, Nicholas C. Curtis, Akiko Watanabe, Hidetaka Tanno, Seungmin Shin, Kevin Ye, Elizabeth Macdonald, Olivia Lavidor, Susan Kong, Tarra Von Holle, et al.Southern Biotech). After three washes, the beads were resuspended in assay buffer and the plates read on a Bio-Plex 3D suspension array system (Bio-Rad). Antigens and controls included BSA, mouse anti-human Ig(κ) (SB81a; Southern Biotech), mouse

(4) Fc receptor engagement of HIV-1 Env-specific antibodies in mothers and infants predicts reduced vertical transmissionFrontiers in ImmunologyDecember 12, 2022Brittani M. Barrows, Shelly J. Krebs, Ningbo Jian, Michelle Zemil, Bonnie M. Slike, Vincent Dussupt, Ursula Tran, Letzibeth Mendez-Rivera, David Chang, Anne Marie O’Sullivan, et al.were washed then resuspended in 40 μL sheath fluid (Luminex Corporation, Austin TX) and analyzed on a Bio-Plex 3D Suspension Array system (Bio-Rad, Hercules CA) running xPONENT v.4.2 (Luminex Corporation, Austin, TX). Antigen-coated microspheres:

(5) Autoreactivity and broad neutralization of antibodies against HIV-1 are governed by distinct mutations: Implications for vaccine design strategiesFrontiers in ImmunologyNovember 18, 2022Xiaojun Li, Dongmei Liao, Zhengyang Li, Jixi Li, Marilyn Diaz, Laurent Verkoczy, Feng Gaobeads were resuspended in assay buffer and fluorescence signals from each microsphere was measured on a Bio-Plex 3D Suspension Array System (Bio-Rad Laboratories, Hercules, CA). Host protein binding assay Binding of recombinant CH103 Ab mutants

(6) Context-dependent effects of IL-2 rewire immunity into distinct cellular circuitsThe Journal of experimental medicineJuly 4, 2022Whyte CE, Singh K, Burton OT, Aloulou M, Kouser L, Veiga RV, Dashwood A, Okkenhaug H, Benadda S, Moudra A, et al.IL-13 levels using ProCartaPlex Immunoassays (Thermo Fisher Scientific) and acquired on the Luminex Bio-Plex 3D Suspension Array System (Bio-Rad). Treg expression of IL-2 in vitro CD4+ Treg and Tconv cells were sorted from Foxp3Thy1.1

(7) Recall of B cell memory depends on relative locations of prime and boost immunizationScience ImmunologyMay 6, 2022Masayuki Kuraoka, Chen-Hao Yeh, Goran Bajic, Ryutaro Kotaki, Shengli Song, Ian Windsor, Stephen C. Harrison, Garnett KelsoeBiotech). After three washes, the beads were re-suspended in assay buffer and the plates were read on a Bio-Plex 3D Suspension Array System (Bio-Rad). The following antigens were coupled with carboxylated beads (Luminex Corp): BSA (Affymetrix),

(8) Low-dose in vivo protection and neutralization across SARS-CoV-2 variants by monoclonal antibody combinationsNature immunologyDecember 1, 2021Dussupt V, Sankhala RS, Mendez-Rivera L, Townsley SM, Schmidt F, Wieczorek L, Lal KG, Donofrio GC, Tran U, Jackson ND, et al.uniquely coded magnetic microspheres (Luminex) per the manufacturer’s protocol. Data were collected on a Bio-Plex 3D Suspension Array system (Bio-Rad) running xPONENT v.4.2 (Luminex). Signal-to-noise ratios were calculated by the dividing the

(9) Silver Nanoparticles Impair Cognitive Functions and Modify the Hippocampal Level of Neurotransmitters in a Coating-Dependent MannerInternational Journal of Molecular SciencesNovember 24, 2021Katarzyna Dziendzikowska, Małgorzata Węsierska, Joanna Gromadzka-Ostrowska, Jacek Wilczak, Michał Oczkowski, Sylwia Męczyńska-Wielgosz, Marcin KruszewskiCA, USA) in the Bio-Plex 200 System (Bio-Rad, Hercules, CA, USA). The final data were analyzed in the Bio-Plex 3D Suspension Array System (Bio-Rad). The assay allows estimating the plasma concentration of granulocyte stimulating factor (G-CSF),

(10) IFNγ Is Critical for CAR T Cell–Mediated Myeloid Activation and Induction of Endogenous ImmunityCancer DiscoverySeptember 1, 2021Darya Alizadeh et al.Panel 11plex (ThermoFisher Scientific) according to the manufacturer’s instructions and acquired on the Bio-Plex 3D Suspension Array System (Bio-Rad Laboratories). Quantitative PCR All primers used for qPCR analysis are listed in Supplementary

(11) Pulmonary mesenchymal stem cells are engaged in distinct steps of host response to respiratory syncytial virus infectionPLOS PathogensJuly 28, 2021Melanie Brügger et al.Human Cytokine 27-plex Assay (Bio-Rad) was used according to the manufacturer’s protocol and read on a Bio-Plex 3D suspension array system including a Bio-Plex Manager software v 6.0 (Bio-Rad). Animals Newborn lambs, males and females were

(12) Synergistic Action of Diclofenac with Endotoxin-Mediated Inflammation Exacerbates Intestinal Injury in VitroACS Infectious DiseasesApril 9, 2021Wen Li Kelly Chen, Emily Suter, Hikaru Miyazaki, Jason Velazquez, Douglas A. Lauffenburger, Linda G. Griffith, Rebecca L. Carriergenerate an 8-point standard curve. Assays were prepared according to manufacturer’s protocol and run on a Bio-Plex 3D Suspension Array System (Bio-Rad Laboratories, Inc.). Data were collected using the xPONENT for FLEXMAP 3D software, version

(13) Human physiomimetic model integrating microphysiological systems of the gut, liver, and brain for studies of neurodegenerative diseasesScience advancesJanuary 1, 2021Trapecar M, Wogram E, Svoboda D, Communal C, Omer A, Lungjangwa T, Sphabmixay P, Velazquez J, Schneider K, Wright CW, et al.diluted the protein stock to generate an eight-point standard curve. Samples were analyzed with the Bio-Plex 3D Suspension Array System (Bio-Rad Laboratories Inc.). Data were collected using the xPONENT for FLEXMAP 3D software, version

(14) RV144 HIV-1 vaccination impacts post-infection antibody responsesPLOS PathogensDecember 8, 2020Thembi Mdluli, Ningbo Jian, Bonnie Slike, Dominic Paquin-Proulx, Gina Donofrio, Aljawharah Alrubayyi, Syna Gift, Rebecca Grande, Mary Bryson, Anna Lee, et al.detection reagent, microspheres are resuspended in 40 μL sheath fluid (Luminex) as above. Data was collected on a Bio-Plex 3D Suspension Array system (Bio-Rad, Hercules CA) running xPONENT v.4.2 (Luminex Corp). Ig subclass and isotype specificity were

(15) Effect of mare’s milk prebiotic supplementation on the gut microbiome and the immune system following antibiotic therapyBiodiversitas Journal of Biological DiversityOctober 8, 2020Madiyar Nurgaziyev et al.bead panel included in a Milliplex MAP kit (Millipore Corp., MA, USA). Levels were measured with a Bio-Plex® 3D suspension array system (Bio-Rad Laboratories, CA, USA). Bioinformatic analysis Bioinformatic analysis was performed in the

(16) Human T Cells Expressing a CD19 CAR-T Receptor Provide Insights into Mechanisms of Human CD19-Positive β Cell DestructionCell reports. MedicineSeptember 22, 2020Ma H, Jeppesen JF, Jaenisch R.(custom assay from R&D to analyze IL2, TNFalpha, INFgamma, GM-CSF, CCL3, CCL4, and CCL5) with a Bio-Rad Bioplex3D Luminex equipment with the cytokine standards and suggested settings provided by the manufacturer. In vitro

(17) Elafin inhibits obesity, hyperglycemia, and liver steatosis in high-fat diet-treated male miceScientific reportsJuly 30, 2020Wang J, Ortiz C, Fontenot L, Mukhopadhyay R, Xie Y, Law IKM, Shih DQ, Mattai SA, Li Z, Koon HW.serum cytokine levels were determined with a 27-plex Multiplex ELISA (#m500kcaf0y, Bio-Rad) using a Bio-plex 3D suspension array system (Bio-Rad) at UCLA Center for Systems Biomedicine64. Human mesenteric fat samples Human mesenteric

(18) Gut-Liver Physiomimetics Reveal Paradoxical Modulation of IBD-Related Inflammation by Short-Chain Fatty AcidsCell systemsMarch 25, 2020Trapecar M, Communal C, Velazquez J, Maass CA, Huang YJ, Schneider K, Wright CW, Butty V, Eng G, Yilmaz O, et al.standard in the same media and serially diluted the protein stock to generate an 8-point standard curve. A Bio-Plex 3D Suspension Array System (Bio-Rad Laboratories, Inc.) was used to analyze the samples. Treg and Th17 cell activation assay To

(19) Cross-Reactivity to Kynureninase Tolerizes B Cells That Express the HIV-1 Broadly Neutralizing Antibody 2F5Journal of immunology (Baltimore, Md. : 1950)December 15, 2019Finney J, Yang G, Kuraoka M, Song S, Nojima T, Verkoczy L, Kitamura D, Haynes BF, Kelsoe G.washes with assay buffer, the beads were suspended in assay buffer and fluorescence was measured with a Bio-Plex 3D Suspension Array System (Bio-Rad). The following antigens were coupled with carboxylated beads (Luminex Corp): goat anti-mouse

(20) Immunization of BLT Humanized Mice Redirects T Cell Responses to Gag and Reduces Acute HIV-1 ViremiaJournal of VirologyOctober 15, 2019Daniel T. Claiborne et al.were determined using the Milliplex human high-sensitivity T cell panel immunoassay kit (Millipore) and read on a Bio-Plex 3D suspension array system (Bio-Rad Laboratories). Coefﬁcient of variation (%CV) was below 30 for all samples. Readings with bead

Be the First to Write a Review!

Multiplex Immunoassays: Five Tips for Optimizing Your Experiments
November 29, 2016
Biomedical and pharmaceutical research increasingly requires concurrent measurement of multiple biomarkers to reveal early indications of disease or response to treatment. Multiplex sandwich immunoassays are frequently ... read more

Watch Video: Bio-Plex® Suspension Array System
The Bio-Plex Suspension Array System is a flexible, easy-to-use multiplex Immunoassay system based ... Read More