Fig 1: The results of the chondrogenic potential of the primary bone-derived cells. (a) Bern score of the toluidine blue-stained chondrogenic pellets for each donor group is determined. No significant differences are obtained between the three donor groups (p = 0.252; general linear model with age as covariate, and a Bonferroni post hoc comparison). Individual samples and means are shown. (b) Representative images of the toluidine blue-stained chondrogenic pellets for each group of donors are shown. Images for all donors are shown in Supplemental Figure S2. (c) von Kossa histology shows no difference in the rate of mineralization between the three donor groups (p >0.05; chi-squared test). Representative images of the von Kossa-stained chondrogenic pellets for each group of donors are shown. Images for all donors are shown in Supplemental Figure S3. (d) Immunofluorescence for the a-1 chain of type II collagen (Col2A1) shows no difference in the presence of the hyaline cartilage between the three donor groups (p > 0.05; chi-squared test). Representative images of the Col2A1-stained chondrogenic pellets for each group of donors are shown. Images for all donors are shown in Supplemental Figure S4. Scale bars, 200 µm. OA, osteoarthritis.