Collagen Type II Antibody 600-401-104-0.5 from Rockland Immunochemicals, Inc.

Product Specs
ItemCollagen Type II Antibody
CompanyRockland Immunochemicals, Inc.
Price Pricing Info Supplier Page View Company Product Page
Catalog Number600-401-104-0.5
Quantity500 µg
ReactivityMammalian, Human, Bovine
ApplicationsELISA,Immunohistochemistry,IF Microscopy,Immunoprecipitation,Western Blot
ImmunogenCollagen Type II from human knee cartilage and bovine nasal cartilage
HostRabbit
Conjugate/TagUnconjugated
FormatLiquid (sterile filtered)
Concentration1.03 mg/mL
ClonalityPolyclonal
PurityCollagen II Antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type II collagens and has negligible cross-reactivity with Type I, III, IV, V or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible
NCBI Gene AliasesAOM, COL11A3, STL1, SEDC, ANFH
Antibody TypePrimary
TargetCollagen Type II
Gene NameCOL2A1
StorageStore vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing

Specifications/Features

Application Note: Anti-Collagen Type II has been tested by dot blot and IHC and is suitable for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, immunohistochemistry, native (non-denaturing, non-dissociating) PAGE, and western blotting for highly sensitive qualitative analysis.

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Rockland Immunochemicals, Inc.

P.O. Box 5199

Limerick, Pennsylvania 19468

United States

Phone: (484) 791-3823
Fax: (484) 369-8654

Company Profile

Website: https://www.rockland.com

(1) Tyrosinase-crosslinked, tissue adhesive and biomimetic alginate sulfate hydrogels for cartilage repairBiomedical materials (Bristol, England)June 24, 2020Öztürk E, Stauber T, Levinson C, Cavalli E, Arlov Ø, Zenobi-Wong M.and imaged with fluorescence microscopy. The primary antibodies used were anti-collagen 2 (Rockland, 600-401104-0.5), anti-proteoglycan hyaluronic acid-binding region (12/21/1-C-6, DSHB), anti-collagen 1 (Abcam, ab6308). Subcutaneous

(2) Is There a Scientific Rationale for the Refixation of Delaminated Chondral Flaps in Femoroacetabular Impingement? A Laboratory StudyClinical orthopaedics and related researchApril 1, 2020Levinson C, Naal FD, Salzmann GM, Zenobi-Wong M, Leunig M.

(3) Human lung microvascular endothelial cells as potential alternatives to human umbilical vein endothelial cells in bio-3D-printed trachea-like structuresTissue & cellApril 1, 2020Taniguchi D, Matsumoto K, Machino R, Takeoka Y, Elgalad A, Taura Y, Oyama S, Tetsuo T, Moriyama M, Takagi K, et al.

(4) Microencapsulation improves chondrogenesis in vitro and cartilaginous matrix stability in vivo compared to bulk encapsulationBiomaterials scienceMarch 17, 2020Li F, Levinson C, Truong VX, Laurent-Applegate LA, Maniura-Weber K, Thissen H, Forsythe JS, Zenobi-Wong M, Frith JE.

(5) Nanocomposite bioink exploits dynamic covalent bonds between nanoparticles and polysaccharides for precision bioprintingBiofabricationMarch 13, 2020Lee M, Bae K, Levinson C, Zenobi-Wong M.

(6) Phenome-based approach identifies RIC1-linked Mendelian syndrome through zebrafish models, biobank associations and clinical studiesNature medicineJanuary 1, 2020Unlu G, Qi X, Gamazon ER, Melville DB, Patel N, Rushing AR, Hashem M, Al-Faifi A, Chen R, Li B, et al.

(7) An injectable heparin-conjugated hyaluronan scaffold for local delivery of transforming growth factor β1 promotes successful chondrogenesisActa biomaterialiaNovember 1, 2019Levinson C, Lee M, Applegate LA, Zenobi-Wong M.

(8) Chondrocytes From Device-Minced Articular Cartilage Show Potent Outgrowth Into Fibrin and Collagen HydrogelsOrthopaedic journal of sports medicineSeptember 1, 2019Levinson C, Cavalli E, Sindi DM, Kessel B, Zenobi-Wong M, Preiss S, Salzmann G, Neidenbach P.

(9) Characterization of polydactyly chondrocytes and their use in cartilage engineeringScientific reportsMarch 12, 2019Cavalli E, Levinson C, Hertl M, Broguiere N, Brück O, Mustjoki S, Gerstenberg A, Weber D, Salzmann G, Steinwachs M, et al.

(10) A selective ER-phagy exerts procollagen quality control via a Calnexin-FAM134B complexThe EMBO journalJanuary 15, 2019Forrester A, De Leonibus C, Grumati P, Fasana E, Piemontese M, Staiano L, Fregno I, Raimondi A, Marazza A, Bruno G, et al.

(11) Cell-Instructive Alginate Hydrogels Targeting RhoABioconjugate chemistrySeptember 19, 2018Formica FA, Cavalli E, Broguiere N, Zenobi-Wong M.

(12) Scaffold-free trachea regeneration by tissue engineering with bio-3D printingInteractive cardiovascular and thoracic surgeryMay 1, 2018Taniguchi D, Matsumoto K, Tsuchiya T, Machino R, Takeoka Y, Elgalad A, Gunge K, Takagi K, Taura Y, Hatachi G, et al.

(13) Intramembranous bone formation after callus distraction is augmented by increasing axial compressive strainPloS oneJanuary 1, 2018Schuelke J, Meyers N, Reitmaier S, Klose S, Ignatius A, Claes L.

(14) Nanoassemblies of Tissue-Reactive, Polyoxazoline Graft-Copolymers Restore the Lubrication Properties of Degraded CartilageACS nanoMarch 28, 2017Morgese G, Cavalli E, Müller M, Zenobi-Wong M, Benetti EM.

(15) Repair of osteochondral defects with in vitro engineered cartilage based on autologous bone marrow stromal cells in a swine modelScientific reportsJanuary 13, 2017He A, Liu L, Luo X, Liu Y, Liu Y, Liu F, Wang X, Zhang Z, Zhang W, Liu W, et al.

(16) Factor XIII Cross-Linked Hyaluronan Hydrogels for Cartilage Tissue EngineeringACS biomaterials science & engineeringDecember 12, 2016Broguiere N, Cavalli E, Salzmann GM, Applegate LA, Zenobi-Wong M.

(17) A Bioinspired Ultraporous Nanofiber-Hydrogel Mimic of the Cartilage Extracellular MatrixAdvanced healthcare materialsDecember 1, 2016Formica FA, Öztürk E, Hess SC, Stark WJ, Maniura-Weber K, Rottmar M, Zenobi-Wong M.

(18) Evaluation of chitosan-GP hydrogel biocompatibility in osteochondral defects: an experimental approachBMC veterinary researchAugust 27, 2014Martins EA, Michelacci YM, Baccarin RY, Cogliati B, Silva LC.

(19) Osteoblast-specific Krm2 overexpression and Lrp5 deficiency have different effects on fracture healing in micePloS oneJanuary 1, 2014Liedert A, Röntgen V, Schinke T, Benisch P, Ebert R, Jakob F, Klein-Hitpass L, Lennerz JK, Amling M, Ignatius A.

(20) Mesenchyme-specific knockout of ESET histone methyltransferase causes ectopic hypertrophy and terminal differentiation of articular chondrocytesThe Journal of biological chemistryNovember 8, 2013Lawson KA, Teteak CJ, Zou J, Hacquebord J, Ghatan A, Zielinska-Kwiatkowska A, Fernandes RJ, Chansky HA, Yang L.

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Rockland Anti-Collagen II Antibody – Immunohistochemistry

Collagen Type II Antibody

Collagen Type II Antibody from Rockland Immunochemicals, Inc.

Description

Specifications/Features

Rockland Immunochemicals, Inc.

Citations (24)

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Collagen Type II Antibody from Rockland Immunochemicals, Inc.

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