Moesin Antibody (MSN/493)

Knockout Validated: Moesin Antibody (MSN/493) [NBP2-44580] - Western blot using lysates from U20S parental cell line and Moesin-1 knockout U20S cell line (KO), collected in RIPA buffer. Nitrocellulose membrane was probed with Mouse Anti-Human/Mouse Moesin Monoclonal Antibody (Catalog # NBP2-44580) at a 1:1000 dilution O/N at 4C, followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody and ECL detection. A specific band was detected for Moesin-1 (as indicated) in the parental U20S cell line, but is not detectable in knockout U20S cell line. The Ponceau stained transfers of each blot are shown to confirm equal protein loading. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

Knockout Validated: Moesin Antibody (MSN/493) [NBP2-44580] - Western blot using lysates from U20S parental cell line and Moesin-1 knockout U20S cell line (KO), collected in RIPA buffer. Nitrocellulose membrane was probed with Mouse Anti-Human/Mouse Moesin Monoclonal Antibody (Catalog # NBP2-44580) at a 1:1000 dilution O/N at 4C, followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody and ECL detection. A specific band was detected for Moesin-1 (as indicated) in the parental U20S cell line, but is not detectable in knockout U20S cell line. The Ponceau stained transfers of each blot are shown to confirm equal protein loading. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

Knockout Validated: Moesin Antibody (MSN/493) [NBP2-44580] - Western blot using lysates from U20S parental cell line and Moesin-1 knockout U20S cell line (KO), collected in RIPA buffer. Nitrocellulose membrane was probed with Mouse Anti-Human/Mouse Moesin Monoclonal Antibody (Catalog # NBP2-44580) at a 1:1000 dilution O/N at 4C, followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody and ECL detection. A specific band was detected for Moesin-1 (as indicated) in the parental U20S cell line, but is not detectable in knockout U20S cell line. The Ponceau stained transfers of each blot are shown to confirm equal protein loading. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).