Goat Anti-Human IgG Antibody, HRP conjugate AP309P from MilliporeSigma

Product Specs
ItemGoat Anti-Human IgG Antibody, HRP conjugate
CompanyMilliporeSigma
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberAP309P
Brand/TrademarkChemicon
Source/Expression SystemAntibodies
ApplicationsVirus detection: Antibodies for serology assay development
Conjugate/Tagperoxidase conjugate
Hostgoat
Reactivityhuman
ClonalityPolyclonal
TargetHuman IgG
Concentration1.0 mg/mL
Clonepolyclonal

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MilliporeSigma

400 Summit Drive

Burlington, Massachusetts 01803

United States

Phone: (800)-645-5476
(800)-645-5439

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Website: www.SigmaAldrich.com/

(1) Characterization of the humanized FRG mouse model and development of an AAV-LK03 variant with improved liver lobular biodistributionMolecular therapy. Methods & clinical developmentMarch 9, 2023Cabanes-Creus M, Navarro RG, Liao SHY, Scott S, Carlessi R, Roca-Pinilla R, Knight M, Baltazar G, Zhu E, Jones M, et al.receiving 50 μL per well of horseradish peroxidase-conjugated anti-human Fc-specific IgG (Chemicon AP309P, diluted 1:10,000 in blocking buffer). Plates were incubated for 1 h at room temperature and washed

(2) A tick C1q protein alters infectivity of the Lyme disease agent by modulating interferon γCell reportsNovember 22, 2022Tang X, Arora G, Matias J, Hart T, Cui Y, Fikrig E.(1:2000, Abcam, #ab6908), and anti-human IgG HRP conjugated secondary antibody (1:2000, Sigma-Aldrich, #AP309P), KPL Sureblue TMB Microwell Peroxidase substrate, 1-component (Seracare, #5120-0077) was added. The

(3) Serum polyethylene glycol-specific IgE and IgG in patients with hypersensitivity to COVID-19 mRNA vaccinesAllergology InternationalOctober 1, 2022Mariko Mouri, Mitsuru Imamura, Shotaro Suzuki, Tatsuya Kawasaki, Yoshiki Ishizaki, Keiichi Sakurai, Hiroko Nagafuchi, Norihiro Matsumura, Marina Uchida, Takayasu Ando, et al.samples were diluted 32-fold. Subsequently, HRP-conjugated goat anti-human IgG antibody (Millipore AP309P) was incubated at 1:100 000 dilution, and the TMB substrate was used for detection. To prepare a standard

(4) Cross-Reactivity of SARS-CoV-2 Nucleocapsid-Binding Antibodies and Its Implication for COVID-19 Serology TestsVirusesSeptember 14, 2022Alexandra Rak, Svetlana Donina, Yana Zabrodskaya, Larisa Rudenko, Irina Isakova-Sivaktimes and an HRP-conjugated goat anti-human IgG secondary antibody (Sigma, St. Louis, MO, USA, cat#AP309P) was added to each well and incubated at 37 °C for 1 h. Then, the plates were thoroughly washed and

(5) Multicenter analysis of neutrophil extracellular trap dysregulation in adult and pediatric COVID-19JCI InsightAugust 22, 2022Carmelo Carmona-Rivera, Yu Zhang, Kerry Dobbs, Tovah E. Markowitz, Clifton L. Dalgard, Andrew J. Oler, Dillon R. Claybaugh, Deborah Draper, Meng Truong, Ottavia M. Delmonte, et al.0.05% PBS-Tween (PBS-T). HRP-conjugated anti-human IgG secondary Ab (1:5000; MilliporeSigma, catalog AP309P) was incubated for 1 hour, at RT, followed by 5 washes with PBS-T. The plate was developed in the presence

(6) Dimerization of SARS-CoV-2 nucleocapsid protein affects sensitivity of ELISA based diagnostics of COVID-19International Journal of Biological MacromoleculesMarch 1, 2022Wajihul Hasan Khan, Nida Khan, Avinash Mishra, Surbhi Gupta, Vikrant Bansode, Deepa Mehta, Rahul Bhambure, M. Ahmad Ansari, Shukla Das, Anurag S. RathoreImmunoblot was then incubated with HRP conjugated-goat anti-human IgG secondary antibodies (Millipore, AP309P) in a dilution of 1:10,000 for 1 h. The immunoblot was washed thrice with 1X TBST and was visualized

(7) The Tissue Distribution of SARS-CoV-2 in Transgenic Mice With Inducible Ubiquitous Expression of hACE2Frontiers in Molecular BiosciencesJanuary 18, 2022Alexander A. Dolskiy et al.washed two times and incubated with secondary rabbit antibodies against human IgG conjugated with HRP (AP309P, Sigma, United States) for 1 h. Signal detection was performed using an AEC substrate (Sigma, United

(8) A human secretome library screen reveals a role for Peptidoglycan Recognition Protein 1 in Lyme borreliosisPLoS pathogensNovember 1, 2020Gupta A, Arora G, Rosen CE, Kloos Z, Cao Y, Cerny J, Sajid A, Hoornstra D, Golovchenko M, Rudenko N, et al.washing and incubating with Goat anti-human IgG HRP conjugated secondary antibody (Sigma-Aldrich, #AP309P) (1:5000), KPL Sureblue TMB Microwell Peroxidase substrate, 1-component (Seracare, #5120–0077) was added.

(9) Novel adenovirus vaccine vectors based on the enteric-tropic serotype 41VaccineMarch 1, 2007Franck Lemiale, Hedi Haddada, Gary J. Nabel, Douglas E. Brough, C. Richter King, Jason G.D. GallProgram, cat. #3957) as a primary antibody and a goat anti-human IgG-HRP conjugate (Chemicon, Cat. #AP309P) as the secondary antibody. 2.4 Animals Female BALB/c (H-2d) mice 8 to 10 weeks of age were purchased

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Goat Anti-Human IgG Antibody, HRP conjugate from MilliporeSigma

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Goat Anti-Human IgG Antibody, HRP conjugate from MilliporeSigma

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