Anti-HA.11 Epitope Tag 901513 from BioLegend

Product Specs
ItemAnti-HA.11 Epitope Tag
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number901513
Quantity200 µL
ApplicationsWestern Blot (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP), Flow Cytometry (FC), Purification
Conjugate/TagUnconjugated
IsotypeIgG1, Kappa
Clone16B12
HostMouse
ImmunogenMonoclonal antibody HA.11 was raised against the twelve amino acid peptide CYPYDVPDYASL
ClonalityMonoclonal
Brand/TrademarkMMS-101R-200
TargetHA.11

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
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Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylationMolecular cellJuly 6, 2023Yiu SPT, Zerbe C, Vanderwall D, Huttlin EL, Weekes MP, Gewurz BE.,Cell Signaling Technology,cat# 5174; RRID: AB_10622025 Anti-HA.11 tag mouse monoclonal antibody (16B12),Biolegend,cat# 901513; RRID: AB_2820200 Anti-NLRP3 rabbit polyclonal antibody,Proteintech,cat# 19771-1-AP; RRID: AB_10646484 Anti-ASC/TMS1

(2) FBXL4 suppresses mitophagy by restricting the accumulation of NIX and BNIP3 mitophagy receptorsThe EMBO JournalJuly 3, 2023Giang Thanh Nguyen‐Dien, Keri‐Lyn Kozul, Yi Cui, Brendan Townsend, Prajakta Gosavi Kulkarni, Soo Siang Ooi, Antonio Marzio, Nissa Carrodus, Steven Zuryn, Michele Pagano, et al.anti‐GFP (B‐2, sc‐9996) were obtained from Santa Cruz Biotechnology. Mouse monoclonal anti‐HA (clone 16B12; 901513) was obtained from BioLegend. Rabbit monoclonal anti‐BNIP3 (clone EPR4034; ab109362: WB) was obtained

(3) Dynamic interplay between RPL3- and RPL3L-containing ribosomes modulates mitochondrial activity in the mammalian heartNucleic acids researchJune 23, 2023Milenkovic I, Santos Vieira HG, Lucas MC, Ruiz-Orera J, Patone G, Kesteven S, Wu J, Feneley M, Espadas G, Sabidó E, et al.tubes and kept at –80°C for subsequent input analysis. A 4 μl aliquot of anti-HA antibody (BioLegend, 901513) was added to the remaining cleared lysate and incubated for 4 h at 4°C on an end-over-end rotator.

(4) Functional gene delivery to and across brain vasculature of systemic AAVs with endothelial-specific tropism in rodents and broad tropism in primatesNature communicationsJune 8, 2023Chen X, Wolfe DA, Bindu DS, Zhang M, Taskin N, Goertsen D, Shay TF, Sullivan EE, Huang SF, Ravindra Kumar S, et al.microtome to 15–30 μm for immunostaining using the following antibodies: mouse anti-HA (Biolegend catalog #901513, diluted 1:1000) and rabbit anti-NeuN (Millipore catalog #ABN78, 1:2000). In a subset of IHC experiments

(5) Altered Signaling and Desensitization Responses in PTH1R Mutants Associated with Eiken SyndromeCommunications BiologyJune 2, 2023Ignacio Portales-Castillo, Thomas Dean, Ross W. Cheloha, Brendan A. Creemer, Jean-Pierre Vilardaga, Sofya Savransky, Ashok Khatri, Harald Jüppner, Thomas J. Gardellaat room temperature with anti-HA mouse monoclonal primary antibody (anti-HA.11, BioLegend, Cat. No. 901513), rinsed, incubated for 1 h at room temperature with a goat-anti-mouse IgG secondary antibody poly-conjugated

(6) RagD auto-activating mutations impair MiT/TFE activity in kidney tubulopathy and cardiomyopathy syndromeNature communicationsMay 15, 2023Sambri I, Ferniani M, Campostrini G, Testa M, Meraviglia V, de Araujo MEG, Dokládal L, Vilardo C, Monfregola J, Zampelli N, et al.dilution 1:1000 IF) and actin AC-74 (# A2228 − 1:5000 WB) were from Sigma-Aldrich; HA.11 epitope tag (cat. 901513 − 1:1000) was from Biolegend; HA clone 3F10 (ref. 1186 7423001 1:800 FACS); Anti-HA High Affinity (ref.11867423001

(7) Evidence for existence of an apoptosis-inducing BH3-only protein, sayonara, in DrosophilaThe EMBO journalApril 17, 2023Ikegawa Y, Combet C, Groussin M, Navratil V, Safar-Remali S, Shiota T, Aouacheria A, Yoo SK.antibody (1:1,000; 3724, Cell Signaling Technology), Anti‐HA.11 Epitope Tag mouse antibody (1:1,000; 901513, BioLegend), Myc‐tag mouse antibody (1:1,000, #2276, Cell Signaling Technology), IRDye® 680RD Goat anti‐Rabbit

(8) Phosphorylation‐linked complex profiling identifies assemblies required for Hippo signal integrationMolecular Systems BiologyApril 12, 2023Federico Uliana, Rodolfo Ciuffa, Ranjan Mishra, Andrea Fossati, Fabian Frommelt, Sabrina Keller, Martin Mehnert, Eivind Salmorin Birkeland, Frank van Drogen, Nevena Srejic, et al.Abcam), anti‐YAP1 (#15407, Santa Cruz), anti‐YAP1phosphoS127 (#4911, Cell Signaling), and anti‐HA (HA.11,901513, BioLegend). Proteins were detected by enhanced chemiluminescence (ECL, Amersham) using horseradish‐peroxidase‐coupled

(9) A novel G protein-biased agonist at the μ opioid receptor induces substantial receptor desensitisation through G protein-coupled receptor kinaseBritish journal of pharmacologyApril 1, 2023Groom S, Blum NK, Conibear AE, Disney A, Hill R, Husbands SM, Li Y, Toll L, Kliewer A, Schulz S, et al.

(10) Aging and memory are altered by genetically manipulating lactate dehydrogenase in the neurons or glia of fliesAging (Albany NY)February 28, 2023Ariel K. Frame, J. Wesley Robinson, Nader H. Mahmoudzadeh, Jason M. Tennessen, Anne F. Simon, Robert C. Cummingfollows: rabbit anti-LDHA (PA5-26531, ThermoFisher Scientific; 1:1000), and mouse anti-HA.11 epitope tag (901513, BioLegend; 1:1000). Subsequently, blots were probed with horse radish peroxidase (HRP) conjugated secondary

(11) Structure of the lysosomal mTORC1–TFEB–Rag–Ragulator megacomplexNatureFebruary 16, 2023Zhicheng Cui, Gennaro Napolitano, Mariana E. G. de Araujo, Alessandra Esposito, Jlenia Monfregola, Lukas A. Huber, Andrea Ballabio, James H. Hurleysc-20011, 1:500 immunofluorescence) were from Santa Cruz; antibody to HA.11 Epitope Tag (catalogue no. 901513) was from Biolegend and HRP-conjugated secondary antibodies to mouse (catalogue no. 401215, 1:5,000

(12) A Novel Single Vector Intersectional AAV Strategy for Interrogating Cellular Diversity and Brain FunctionbioRxiv : the preprint server for biologyFebruary 8, 2023Hughes AC, Pollard BG, Xu B, Gammons JW, Chapman P, Bikoff JB, Schwarz LA.Antibody,Species,Dilution,Source TH,Rabbit,1:1000,EMD Millipore (ab152) TH,Mouse,1:1000,EMD Millipore (MAB318) HA.11,Mouse,1:1000,BioLegend (901513) cFos,Rabbit,1:1000,Synaptic Systems (226008) GFP,Chicken,1:1000,Aves Labs (GFP3717982) GFP,Rabbit,1:1000,Invitrogen

(13) Identification of mosquito proteins that differentially interact with alphavirus nonstructural protein 3, a determinant of vector specificityPLOS Neglected Tropical DiseasesJanuary 25, 2023Nathaniel M. Byers, Paul L. Burns, Olga Stuchlik, Matthew S. Reed, Jeremy P. Ledermann, Jan Pohl, Ann M. Powers(Thermofisher T2015) + 0.1% Tween20 was used for blocking and antibody dilution. Anti-HA (Biolegend 901513) diluted 1:1000 and anti-actin (BD Biosciences 612656) diluted 1:3333 were used. An alkaline phosphatase

(14) Functional gene delivery to and across brain vasculature of systemic AAVs with endothelial-specific tropism in rodents and broad tropism in primatesbioRxivJanuary 18, 2023Xinhong Chen, Damien A. Wolfe, Dhanesh Sivadasan Sivadasan Bindu, Mengying Zhang, Naz Taskin, David Goertsen, Timothy F. Shay, Erin Sullivan, Sheng-Fu Huang, Sripriya Ravindra Ravindra Kumar, et al.microtome to 15-30 μm for immunostaining using the following antibodies: mouse anti-HA (Biolegend catalog #901513, 1:1000) and rabbit anti-NeuN (Millipore catalog #ABN78, 1:2000). In a subset of IHC experiments we

(15) A Live Cell Protein Complementation Assay for ORFeome-Wide Probing of Human HOX InteractomesCellsJanuary 3, 2023Jia Y, Reboulet J, Gillet B, Hughes S, Forcet C, Tribollet V, Hajj Sleiman N, Kundlacz C, Vanacker JM, Bleicher F, et al.Abcam, Paris, France), anti-GFP (rabbit) (1:2000, A11122, Invitrogen, France), anti-HA (mouse) (1:3000, 901513, Biolegend, San Diego, CA, USA) and anti-HOXB13 (rabbit) (1:1000, PA5-98698, Invitrogen, France). All

(16) Signal sequences encode information for protein folding in the endoplasmic reticulumThe Journal of cell biologyJanuary 2, 2023Sun S, Li X, Mariappan M.Laboratory of Molecular Biology, Cambridge, UK). Rabbit α-BiP (#11587-1-AP; Proteintech), Mouse α-HA (#901513; Biolegend), Mouse α-PDI (#MA3-018; Affinity Bioreagents), Goat α-Rat-HRP (#7077; Cell Signaling), Goat

(17) Rpl3l gene deletion in mice reduces heart weight over timeFrontiers in physiologyJanuary 1, 2023Grimes KM, Prasad V, Huo J, Kuwabara Y, Vanhoutte D, Baldwin TA, Bowers SLK, Johansen AKZ, Sargent MA, Lin SJ, et al.antibodies were diluted in blocking buffer and then applied to the sections overnight at 4°C: HA (BioLegend #901513 at 1:500) and desmin (Abcam, ab15200 at 1:300). The next day, the sections were washed in 1x PBS and

(18) UCP2-dependent redox sensing in POMC neurons regulates feedingCell ReportsDecember 27, 2022Nal Ae Yoon, Sungho Jin, Jung Dae Kim, Zhong Wu Liu, Qiushi Sun, Rebecca Cardone, Richard Kibbey, Sabrina DianoRRID:AB_514500 Donkey anti-rabbit IgG,Jackson ImmunoResearch,Cat# 711-005-152; RRID:AB_2340585 HA .11,BioLegend,Cat# 901513; RRID:AB_2565335 GFP,Abcam,Cat# ab13970; RRID:AB_300798 ,, Bacterial and virus strains,Bacterial

(19) Intramembrane client recognition potentiates the chaperone functions of calnexinThe EMBO journalDecember 15, 2022Bloemeke N, Meighen-Berger K, Hitzenberger M, Bach NC, Parr M, Coelho JP, Frishman D, Zacharias M, Sieber SA, Feige MJ.mouse monoclonal (M2) anti‐FLAG (F1804, Sigma‐Aldrich) at 1:1,000; mouse monoclonal (16B12) anti‐HA (901513, Biolegend) at 1:500; mouse monoclonal (C8.B6) anti‐calnexin (MAB3126, Chemicon) at 1:1,000; monoclonal

(20) PARG and BRCA1–BARD1 cooperative function regulates DNA repair pathway choice during gametogenesisNucleic Acids ResearchDecember 8, 2022Shalini Trivedi, Jitka Blazícková, Nicola Silvacytological analyses were: mouse anti-GFP 1:500 (Roche, #11814460001), mouse anti-HA 1:600 (Biolegend, #901513), rabbit anti-HA pre-absorbed against untagged animals 1:100 (Sigma, #H6908), rabbit anti-OLLAS tag

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(1)

1/27/2022Anti-HA.11 Antibody from BioLegend Isabelle da CostaThis is an exceptional antibody for both immunoblotting and immunofluorescence assays of Ha-tagged proteins.
(1)

8/1/2016HA Tag Antibody Is Excellent For ICC Timur MavlyutovGoal of this research is finding specific anti-HA antibody. After transfection of HA-CAS to PC12 cells, specific anti-HA signal was detected in the cell nuclei of a few transfected cells. Thus, ... Read Review
(1)

7/7/2016Extremely Reliable Anti-HA Antibody Nico MeyerWe analyzed samples of crude Arabidopsis thaliana (mouse-ear cress) protein extract from seedlings or later developmental stages according to their protein content. HA tagged proteins in Arabidopsis ... Read Review
(1)

10/6/2015Anti-HA (16B12) Biolegend Rodrigo Orlandini de CastroU2OS cells transfected with HA-SceI were lysed in sample buffer and proteins were separated in 4-20% gradient SDS-PAGE. Ha-SceI expression was probed by anti-HA (16B12) (1:1000) from BioLegend. The ... Read Review
(1)

9/24/2015Excellent Antibody for Detecting HA Epitope Tag Samyabrata BhaduriThis antibody detects HA epitope tagged proteins with great ease in a variety of human and mouse cell types.

Anti-HA.11 Epitope Tag from BioLegend

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Anti-HA.11 Epitope Tag from BioLegend

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