Fig 1: Depletion of 4E-BP1 in hMSCs reduces the levels of core components of the mitochondrial respiration complex III. (A) Schematic diagram of quantitative proteomic analysis. (B) Gene Ontology Biological Process (GO-BP) enrichment analysis of differentially expressed proteins (DEPs) between EIF4EBP1+/+ and EIF4EBP1-/- hMSCs (P4). (C) GSEA of a statistically significant gene set, oxidative phosphorylation, in EIF4EBP1-/- hMSCs compared with EIF4EBP1+/+ hMSCs (P4). (D) Gene Ontology Cellular Component (GO-CC) enrichment analysis of DEPs between EIF4EBP1+/+ and EIF4EBP1-/- hMSCs (P4). (E) Boxplots showing the scaled protein abundance of DEPs of mitochondrial complexes I, III, and IV in EIF4EBP1-/- hMSCs compared with EIF4EBP1+/+ hMSCs (P4). n = 3 biological replicates. Two-tailed unpaired t-test. (F) Western blot analysis of components of mitochondrial respiration complexes I, II, III, IV, V using a total OXPHOS human WB antibody cocktail (Abcam ab110411) in EIF4EBP1+/+ and EIF4EBP1-/- hMSCs (P4). Statistical data are presented as means ± SEM. n = 3 biological replicates. Two-tailed unpaired t-test. (G) Western blot analysis of UQCRC2, UQCRB and UQCRFS1 using independent antibodies in EIF4EBP1+/+ and EIF4EBP1-/- hMSCs (P4). Antibodies for UQCRC2 (Santa sc-390378), UQCRB (Abcam ab190360) and UQCRFS1 (Santa sc-271609) were utilized for targeting UQCRC2, UQCRB and UQCRFS1, respectively. Statistical data are presented as means ± SEM. n = 3 biological replicates. Two-tailed unpaired t-test.
Supplier Page from Abcam for Total OXPHOS Human WB Antibody Cocktail