alpha Tubulin Antibody 32-2500 from Thermo Fisher Scientific

Product Specs
Itemalpha Tubulin Antibody
CompanyThermo Fisher Scientific
Price Pricing Info Supplier Page View Company Product Page
Catalog Number32-2500
Quantity100 µg
ApplicationsImmunocytochemistry
Conjugate/TagUnconjugated
FormatPBS with 0.1% sodium azide; pH 7.4
ReactivityCanine, Human, Mouse, Non-human primate, Rat, Sea urchin
Targetalpha Tubulin
IsotypeIgG1
CloneB-5-1-2
HostMouse
ImmunogenSarkosyl-resistant filament from sea urchin sperm axonemes
NCBI Full Gene Nametubulin, alpha-like 3, tubulin, alpha 1A, tubulin, alpha 1C, tubulin, alpha 1B, tubulin alpha 3c, tubulin alpha 1b, tubulin alpha 3e, tubulin alpha 1c, tubulin alpha 1a, tubulin beta 2B class IIb, tubulin, beta 2B class IIB, tubulin, beta 2B class IIb, tubulin, alpha 4A, tubulin alpha 4a, tubulin alpha like 3
NCBI Gene Aliases2410129E14Rik, RGD1309427, K-ALPHA-1, TUBA3, TUBA2, TUBA1, LIS3, TUBA6, Tuba4, PMGYSA, Tuba2, TUBA1A, Tuba6, RGD1565476, brdp, H2-ALPHA, bA408E5.3, bA506K6.1, M[a]6, M[a]4, TUBB2B, CDCBM7, Tubb2, Tuba-1, ALS22, bcm948, B-ALPHA-1, Tuba1
ClonalityMonoclonal
Concentration0.5 mg/mL
Storage Temperature-20°C
PurityProtein A
Antibody TypePrimary
Storage-20°C

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

Company Profile

Website: www.thermofisher.com

(1) Three-dimensional structured illumination microscopy with enhanced axial resolutionNature biotechnologySeptember 1, 2023Li X, Wu Y, Su Y, Rey-Suarez I, Matthaeus C, Updegrove TB, Wei Z, Zhang L, Sasaki H, Li Y, et al.

(2) Strategic self-limiting production of infectious HIV particles by CRISPR in permissive cellsMolecular Therapy - Nucleic AcidsJune 13, 2023Hong Liu, Chen Chen, Shuren Liao, Danielle K. Sohaii, Conrad R.Y. Cruz, Tricia H. Burdo, Thomas J. Cradick, Anand Mehta, Carlos Barrero, Magda Florez, et al.(sc-374006; Santa Cruz Biotechnology, Dallas, TX), gp160 (13390; NIH AIDS Reagent Program), α-tubulin (32-2500; Life Technologies), HIV (3957; NIH AIDS Reagent Program), HIV-1 gp120 (2343; NIH AIDS Reagent Program),

(3) Understanding In Vitro Pathways to Drug Discovery for TDP-43 ProteinopathiesInternational journal of molecular sciencesNovember 25, 2022Cheng HWA, Callis TB, Montgomery AP, Danon JJ, Jorgensen WT, Ke YD, Ittner LM, Werry EL, Kassiou M.Merck), mouse anti-TIA (sc-48371, 1:500, Santa Cruz Biotechnology, Dallas, TX, USA), mouse anti-tubulin (32–2500, 1:5000, Thermo Fisher Scientific), rabbit anti-lamin B1 (ab16048, 1:5000, Abcam, Cambridge, UK). Blots

(4) Incorporating the image formation process into deep learning improves network performanceNature methodsNovember 1, 2022Li Y, Su Y, Guo M, Han X, Liu J, Vishwasrao HD, Li X, Christensen R, Sengupta T, Moyle MW, et al.

(5) The localization of PHRAGMOPLAST ORIENTING KINESIN1 at the division site depends on the microtubule-binding proteins TANGLED1 and AUXIN-INDUCED IN ROOT CULTURES9 in ArabidopsisThe Plant CellOctober 27, 2022Alison M Mills, Victoria H Morris, Carolyn G Rasmussen1:2,000 dilution of monoclonal anti-α-tubulin B-5-1-2 antibody (Life Technologies, Carlsbad, CA, USA; 32–2500) followed by 1:2000 dilution of Alexa-568 goat anti-mouse antibody (Thermo Fisher, Waltham, MA, USA;

(6) Modest increase of KIF11 expression exposes fragilities in the mitotic spindle, causing chromosomal instabilityJournal of cell scienceSeptember 1, 2022Dale KL, Armond JW, Hynds RE, Vladimirou E.reversine (Sigma-Aldrich). Antibodies were used as follows: mouse α-tubulin (Thermo Fisher Scientific, 32-2500), 1:5000 (western blotting or WB) and 1:500 (immunofluorescence or IF); rabbit α-tubulin (Abcam, ab52866),

(7) The receptor kinase SRF3 coordinates iron-level and flagellin dependent defense and growth responses in plantsNature communicationsAugust 1, 2022Platre MP, Satbhai SB, Brent L, Gleason MF, Cao M, Grison M, Glavier M, Zhang L, Gaillochet C, Goeschl C, et al.milk). The same membrane was re-blotted with Tubulin antibody as the internal control (Invitrogen™, Cat. 32-2500, 1:5000 in 5% non-fat milk; Goat Anti-Mouse IgG (H + L)-HRP Conjugate (Bio-Rad, Cat. No. 170-6516) in

(8) First Modern Data on the Lophophore Nervous System in Adult Novocrania anomala and a Current Assessment of Brachiopod PhylogenyBiology (Basel)March 6, 2022Elena Temerevaspecimens were incubated overnight in primary antibody (mouse anti-acetylated-a-tubulin; 1:700; cat. #32-2500; ThermoFischer) in PBT at 4 °C, washed in PBT three times for 5 h each, and exposed to the secondary

(9) Biallelic mutations in MOS cause female infertility characterized by human early embryonic arrest and fragmentationEMBO molecular medicineDecember 7, 2021Zhang YL, Zheng W, Ren P, Hu H, Tong X, Zhang SP, Li X, Wang H, Jiang JC, Jin J, et al.Abcam), rabbit anti‐Vinculin (1:1,000, ab207440, Abcam), and mouse polyclonal anti‐α‐tubulin (1:1,000, 32‐2500, Thermo Fisher Scientific). Co‐immunoprecipitation Co‐immunoprecipitation assays were performed as

(10) Expansion Microscopy Reveals Plasmodium falciparum Blood-Stage Parasites Undergo Anaphase with A Chromatin Bridge in the Absence of Mini-Chromosome Maintenance Complex Binding ProteinMicroorganismsNovember 6, 2021Liffner B, Absalon S.antibodies were used in this study: Mouse IgG1 anti-alpha Tubulin Clone B-5-1-2; ThermoFisher cat. No. 32-2500 (1:1000 unexpanded samples, 1:500 U-ExM samples), Mouse IgG2a anti-Centrin-1 Clone 20H5; EMD Millipore

(11) An immune-competent, replication-permissive Syrian Hamster glioma model for evaluating Delta-24-RGD oncolytic adenovirusNeuro-oncologyNovember 2, 2021Phillips LM, Li S, Gumin J, Daou M, Ledbetter D, Yang J, Singh S, Parker Kerrigan BC, Hossain A, Yuan Y, et al.

(12) PLEKHA5, PLEKHA6, and PLEKHA7 bind to PDZD11 to target the Menkes ATPase ATP7A to the cell periphery and regulate copper homeostasisMolecular Biology of the CellNovember 1, 2021Sophie Sluysmans, Isabelle Méan, Tong Xiao, Amina Boukhatemi, Flavio Ferreira, Lionel Jond, Annick Mutero, Christopher J. Chang, Sandra Citibrain sections); mouse β-tubulin (32-2600; Thermo Fisher Scientific; IB: 1/3500); mouse α-tubulin (32-2500; Thermo Fisher Scientific; IF: 1/250); guinea pig α-tubulin (AA345 scFv-F2C; in house [ Guerreiro and

(13) Volumetric interferometric lattice light-sheet imagingNature biotechnologyNovember 1, 2021Cao B, Coelho S, Li J, Wang G, Pertsinidis A.PBS) for 10 min and stained with primary antibodies (Mouse anti-α-tubulin, Thermo Fisher Scientific 32-2500, 1/100 dilution; Rabbit anti-Tomm20, Sigma HPA011562, 1/50 dilution) for 2 hr in blocking buffer. The

(14) The peroxisomal transporter ABCD3 plays a major role in hepatic dicarboxylic fatty acid metabolism and lipid homeostasisJournal of inherited metabolic diseaseNovember 1, 2021Ranea-Robles P, Chen H, Stauffer B, Yu C, Bhattacharya D, Friedman SL, Puchowicz M, Houten SM.Cell Signaling), anti-ACC (3662, Cell Signaling), anti-MLYCD (SAB2702043, Sigma), and anti-α-tubulin (32-2500, Thermo Fisher). The anti-MVK antibody was a gift from Dr. Hans Waterham (AMC, Amsterdam) (Hogenboom

(15) Peroxisomal L-bifunctional protein (EHHADH) deficiency causes male-specific kidney hypertrophy and proximal tubular injury in miceKidney360September 1, 2021Ranea-Robles P, Portman K, Bender A, Lee K, He JC, Mulholland DJ, Argmann C, Houten SM.Novus Bio), anti-CPT2 (26555–1-AP, Proteintech), anti-MCAD (55210–1-AP, Proteintech), anti-α-tubulin (32–2500, Thermo Fisher), anti-citrate synthase (GTX628143, Genetex), and anti-AMACR 16. RNA-seq, differential

(16) Site-Specifically-Labeled Antibodies for Super-Resolution Microscopy Reveal In Situ Linkage ErrorsACS NanoJuly 27, 2021Susanna M. Früh, Ulf Matti, Philipp R. Spycher, Marina Rubini, Sebastian Lickert, Thomas Schlichthaerle, Ralf Jungmann, Viola Vogel, Jonas Ries, Ingmar Schoenanti-mouse IgG (Jackson Immuno Research, ref: 715-005-151); mouse anti-β-tubulin (ThermoFisher Scientific, 32-2500). Other IgGs used for testing the conjugation protocol are listed in Supplementary Table S1. First,

(17) Murine deficiency of peroxisomal L-bifunctional protein (EHHADH) causes medium-chain 3-hydroxydicarboxylic aciduria and perturbs hepatic cholesterol homeostasisCellular and molecular life sciences : CMLSJuly 1, 2021Ranea-Robles P, Violante S, Argmann C, Dodatko T, Bhattacharya D, Chen H, Yu C, Friedman SL, Puchowicz M, Houten SM.anti-HMGCR (AMAb90618, Atlas Antibodies), anti-citrate synthase (GTX628143, Genetex), and anti-α-tubulin (32–2500, Thermo Fisher). Anti-MVK and anti-PMVK antibodies were a gift from Dr. Hans Waterham (AMC, Amsterdam)

(18) Combined Targeting of PARG and Wee1 Causes Decreased Cell Survival and DNA Damage in an S-Phase-Dependent MannerMolecular cancer research : MCRFebruary 1, 2021Agostini LC, Jain A, Shupp A, Nevler A, McCarthy G, Bussard KM, Yeo CJ, Brody JR.γH2A.X (ser139) (#9718), Wee1 (#4936), pCDK1 (Y15) (#911S), from Cell Signaling Technology, α-Tubulin (#32–2500) and β-actin (#MA5–15739) from Invitrogen. All primary antibodies were followed by LI-COR IR Dye secondary

(19) WW, PH and C-Terminal Domains Cooperate to Direct the Subcellular Localizations of PLEKHA5, PLEKHA6 and PLEKHA7Frontiers in cell and developmental biologyJanuary 1, 2021Sluysmans S, Méan I, Jond L, Citi S.2016), IF: 1:500); mouse β-tubulin (32-2600, Thermo Fisher Scientific, IB: 1:3500); mouse α-tubulin (32-2500, Thermo Fisher Scientific, IF: 1:250); guinea pig α-tubulin (AA345 scFv-F2C, Geneva Antibody Facility,

(20) Modulation of protein phosphatase 1 gamma 2 during cell division of cervical cancer HeLa cellsContemporary oncology (Poznan, Poland)January 1, 2021Agnihotri SK, Kar P, Singh M, Pant G, Mitra K, Bhatt MLB, Sachdev M.(25°C) followed by incubation with PP1γ2 specific primary antibody at a dilution of 1:250 and α-Tubulin (32-2500, Invitrogen)-specific primary antibody at a dilution of 1:500 overnight (16 hrs) at 4°C. After incubation,

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alpha Tubulin Antibody

alpha Tubulin Antibody from Thermo Fisher Scientific

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alpha Tubulin Antibody from Thermo Fisher Scientific

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