Fig 1: Par3 localization requires Lgl in S2 cells.(A) Endogenous expression of Lgl in S2 cells stained with anti-Lgl and DAPI at 2 days post-transfection of the empty vector. (B) Par3 and endogenous Lgl localize complementarily in 71% of cells (n = 24) where overexpressed Par3 was asymmetrically localized. Arrow, Par3 crescent. Arrowhead, Lgl. (C) Live-imaging of myc-Par3-mKates without (left) or with (right) Lgl knockdown by RNAi at 2 days post-transfection. (D) S2 cells over-expressing flag-Par3 and myc-Lgl3A, stained with anti-flag-tag, anti-myc-tag and DAPI. Lgl3A was cortically uniform in contrast to cytoplasmic Par3 distribution. (E) Live-imaging of myc-Par3-mKates and Par6-GFP with Lgl knockdown by RNAi at 2 days post-transfection. Myc-Par3-mKate2 co-localized with Par6-GFP in 100% of cells (n = 109) where the overexpressed Par3 was uniformly distributed. (F) Live-imaging of myc-Par3-mKates and aPKC-GFP with Lgl knockdown by RNAi at 2 days post-transfection. Myc-Par3-mKate2 co-localized with aPKC-GFP in 97.8% of cells (n = 137) where overexpressed Par3 was uniformly distributed.