Description
Product Characteristics:
Translation is carried out by the ribosome and several associated protein factors through three consecutive steps: initiation, elongation and termination. Termination of protein synthesis takes place when the ribosomal A site is occupied simultaneously by one of three stop codons and by a class 1 translation termination factor. In eukaryotes, this termination factor is the eukaryotic release factor 1 (eRF1), a protein that promotes hydrolysis of the last peptidyl-tRNA on the ribosome. eRF1 activity is stimulated by the association with the GTP-binding protein eRF3. eRF1 forms a quaternary complex with eRF3, GTP and the ribosome. This complex performs a dual role, where, in the GTP state,??? ??? ??? it controls the positioning of eRF1 toward the stop codon and peptidyl-tRNA, and, in the GDP state,??? ??? ??? it promotes the release of the eRFs from the ribosome. eRF1 contains a highly conserved Asn-Ile-Lys-Ser (NIKS) tetrapeptide, which is essential for the interaction of eRF1 with the ribosome. The gene encoding human eRF1 maps to chromosome 5q31.2.
Subcellular location: Cytoplasm
Synonyms: Cl1 protein, D5S1995, ERF, eRF1, ERF1_HUMAN, ETF1, Eukaryotic peptide chain release factor subunit 1, Eukaryotic release factor 1, Eukaryotic translation termination factor 1, MGC111066, Polypeptide chain release factor 1, Protein Cl1, RF1, Sup45 yeast omnipotent suppressor 45 homolog like 1, SUP45L1, TB3 1, TB3-1.
Target Information: This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in coding and non-coding transcript variants. Pseudogenes of this gene are found on chromosomes 5, 7 and X. [provided by RefSeq, Jan 2012]