Fig 2: Summary of the role of AFSC-CM in experimental NEC.NEC induced an intestinal damage, increased inflammation (IL-6 and TNF-a), neutrophil infiltration (MPO), and elevated apoptosis (CC3), as well as decreased angiogenesis or vascularization (VEGF). Further, it diminished epithelial proliferation (Ki67) and stem cell regeneration activity (Lgr5 and Olfm4), however, CM administration reverses all of these effects to yield reduced intestinal injury and improved survival.

Fig 3: NEC survival and intestinal injury.(A) Survival curves for mice pups from the 3 groups on postnatal days 5 to 9. (B) Representative histological images of the terminal ileum. (C) Histological score of intestinal injury. Inflammatory gene marker expression of (D) Il-6 and (E) Tnf-a. Immunofluorescent protein expression of (F) MPO and (G) CC3 in the terminal ilea of the 3 groups. Yellow arrows indicate positive staining. Relative quantification of the (H) MPO and (I) CC3 protein expressions. (J) Angiogenesis/vascularization Vegf gene expression in the 3 groups. Data are presented as mean ± standard error, with significance of group comparisons based on one-way ANOVA and Tukey post-hoc tests. n = 10 for each group, *p<0.05, **p<0.01, and ***p<0.001.

Fig 4: Scatter dot plot of the percentage of double-positive ADAM8/marker cells as analyzed in 10 representative PDAC sections stained for CD68 (•), CD3 (¦), CD4 (?), CD8 (?), CD56 (?), SMA (?) and MPO (?). Note that the frequency of ADAM8+ stromal cells is highest for macrophages (CD68), NK cells (CD56), and neutrophils (MPO). For each section analyzed, data are derived from quantification of 5 viewing fields in the tumor proximal stroma areas. Median values with interquartile ranges are indicated. Note that the highest frequency of co-localization of ADAM8 with stromal markers is observed for MPO (neutrophils).

Fig 5: FXII is expressed by skin and blood neutrophils in FACAS.a Representative image from serial sections of the lesional skin of FACAS patient ca. 1 h after cold exposure. FXII immunoreactivity is restricted to neutrophils at perivascular sites and within blood vessels (white arrows), n = 2 technical replicates. b Exemplary images from cytospins isolated from the peripheral blood of FACAS patients and healthy control subjects. Only single FACAS neutrophils (0.72%), but not healthy control neutrophils (0%), present with double-positive cytoplasmatic staining for FXII and MPO. Original magnification ×400, n = 3 technical replicates. c Peripheral blood mononuclear cells were isolated from healthy control subjects by density-gradient centrifugation and stimulated with increasing doses of bradykinin. IL-8 secretion was assessed by ELISA. Pooled data of n = 3 different donors, n = 2 technical replicates. The graph shows individual data points and the mean ± s.e.m. Statistical significance is indicated by one-way ANOVA with Sidak’s multiple comparisons. Source data are provided as a source data file.