Fig 2: KDM5B inhibitor AS-8351 attenuates tumor growth of Ewing sarcoma cells in vivo.A Xenograft tumors extracted from mice models. B Box plot of xenograft tumor weight between the control and AS-8351 treatment groups. C Tumor growth curves of the xenograft tumors. Blue arrows indicate that AS-8351 is being used. D Mice weight curves during the experiment. Blue arrows indicate that AS-8351 is being used. E Representative immunohistochemistry images of Ki-67 and CD99 protein expression in xenografts at the end of the experiment. Ki-67 is used to assess cell proliferation. CD99, typically expressed on the cell membrane of Ewing sarcoma, is used to identify cancer cells from the stroma. Scale bars: 50 µm. Error bars in B, C, D indicate standard deviation. P values in B are determined by two-tailed unpaired Student’s t test. P values in C and D are determined by two-way ANOVA test. *Above the bars indicates significant difference; ****P < 0.0001; n.s. represents no significance.