Fig 1: GIGYF2 protein interacts with p97/VCP, and GIGYF deletion affects the UPS in human cells(A) Growth of cells lacking both GIGYF1 and 2 (??), measured in Incucyte. Representative of 3 biological replicates; data are average of 3 independent wells.(B) Western blot showing the effect of GIGYF1/2 double deletion (??) on the level of poly-ubiquitylated proteins in the absence and presence of proteasome inhibitor (MG132), before or after UV irradiation.(C) Top, western blot of coIP of p97/VCP with GIGYF2 from WT or GIGYF1/2 double-knockout cells (??). Bottom, inputs.(D) Representative images from proximity ligation assays (PLAs), using the cells and antibodies indicated. White scale bar: 10 µm.(E) Graph showing PLA signals per cell from (D) in WT or ?? cells with antibody combinations shown below. Mean and SD shown in black. ****p < 0.0001 following one-way ANOVA and Dunnett’s multiple comparisons. 69 cells analyzed in each condition. Representative of 2 biological replicates.(F) Western blot of GIGYF1 and 2 after subcellular fractionation. Tubulin and histone mark the cytoplasm and chromatin, respectively.