ELISA demonstrating the specificity of Human Anti-Ustekinumab Antibody, clone AbD17829 (HCA208) using various antigens as coating reagents (A: Human serum, B: human IgG1/kappa from myeloma plasma, C: Rituximab, D: Bevacizumab, E: Adalimumab, F: Alemtuzumab, G: Infliximab, H: Ustekinumab).
Antigens were coated at 5 μg/mL on a microtiter plate overnight. After washing and blocking with 5% BSA, the anti-Ustekinumab antibody, clone AbD17829 (20μL from a 2μg/mL solution) was added followed by Mouse anti-V5-tag:HRP in HiSPEC buffer . Detection was performed using a fluorogenic peroxidase substrate.

ELISA demonstrating the specificity of Human Anti-Ustekinumab Antibody, clone AbD17829 (HCA208) using various antigens as coating reagents (A: Human serum, B: human IgG1/kappa from myeloma plasma, C: Rituximab, D: Bevacizumab, E: Adalimumab, F: Alemtuzumab, G: Infliximab, H: Ustekinumab).
Antigens were coated at 5 μg/mL on a microtiter plate overnight. After washing and blocking with 5% BSA, the anti-Ustekinumab antibody, clone AbD17829 (20μL from a 2μg/mL solution) was added followed by Mouse anti-V5-tag:HRP (MCA1360P) in HiSPEC buffer (BUF049). Detection was performed using a fluorogenic peroxidase substrate.