Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596) antibody ab61791 from Abcam

Product Specs
ItemAnti-Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596) antibody
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab61791
Antibody TypePrimary
HostRabbit
ApplicationsELISA, Immunocytochemistry/ Immunofluorescence, Western blot
ClonalityPolyclonal
Quantity100 UG
IsotypeIgG
Conjugate/TagUnconjugated
FormatThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site
Concentration1 mg/ml
ModificationsPhospho-Y596
Research AreaImmuno-oncology
Gene NameEPHB1
ReactivityHuman
TargetEph receptor B1 + Eph receptor B2

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Age-related decline in hippocampal tyrosine phosphatase PTPRO is a mechanistic factor in chemotherapy-related cognitive impairmentJCI InsightJuly 24, 2023Zhimeng Yao, Hongmei Dong, Jianlin Zhu, Liang Du, Yichen Luo, Qing Liu, Shixin Liu, Yusheng Lin, Lu Wang, Shuhong Wang, et al.Cell Signaling Technology), SRC (1:100; catalog sc-8056, Santa Cruz Biotechnology), p-EPHB2 (1:400; catalog ab61791, Abcam), EPHB2 (1:200; catalog 83029, Cell Signaling Technology), p-EPHA4 (1:100; catalog EP2731, ECM Biosciences

(2) Cell-cell contact-driven EphB1 cis- and trans- signalings regulate cancer stem cells enrichment after chemotherapyCell death & diseaseNovember 19, 2022Wang L, Peng Q, Xie Y, Yin N, Xu J, Chen A, Yi J, Shi W, Tang J, Xiang J.reagents Antibodies to proteins were obtained from the following sources: EphB1 (#ab129103) and phos-EphB1 (#ab61791) for Western blot were purchased from Abcam; EphB1 (#AF542) for immunohistochemistry was purchased from

(3) Tracheal separation is driven by NKX2-1-mediated repression of Efnb2 and regulation of endodermal cell sortingCell reportsMarch 15, 2022Lewis AE, Kuwahara A, Franzosi J, Bush JO.EPHB4 (1:75, R&D, AF446), phospho-EPHA2+A3+A4 (1:150, Abcam, ab62256), phospho-EPHB1+B2 (1:150, Abcam, ab61791), GFP (1:500, Abcam, ab13970), KRT5 (1:250, BioLegend, 905501), KRT8 (1:75, DSHB, AB_531826), LRIG1

(4) Key role for EphB2 receptor in kidney fibrosisClinical ScienceSeptember 17, 2021Zhimin Huang, Simeng Liu, Anna Tang, Laith Al-Rabadi, Mark Henkemeyer, Patrice N. Mimche, Yufeng HuangAF467 (cat. # AF467, R&D Systems, Minneapolis, MN, U.S.A.), rabbit anti-phospho-EphB1/EphB2Y594+Y596 ab61791 (Abcam, Cambridge, U.K.), rat anti-mouse F4/80 IgG (Cl:A3-1, Bio-Rad Laboratories, Inc., Hercules, CA,

(5) Peripheral EphrinB1/EphB1 signalling attenuates muscle hyperalgesia in MPS patients and a rat model of taut band-associated persistent muscle painMolecular PainDecember 28, 2020Feihong Jin, Lianying Zhao, Qiya Hu, Feng Qifollowing primary antibodies: anti-Eph receptor B1 and Eph receptor B2 (phospho Y594 and Y604) (Abcam, ab61791, 1:1000). The membranes were washed with Tris-buffered saline Tween-20 (TBST) and incubated for 1.5

(6) The pathophysiological nature of sarcomeres in trigger points in patients with myofascial pain syndrome: A preliminary studyEuropean Journal of PainNovember 1, 2020Feihong Jin, Yaqiu Guo, Zi Wang, Ahmed Badughaish, Xin Pan, Li Zhang, Feng Qiantibodies at 4°C overnight: anti‐Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y604) antibody (Abcam ab61791 1:300), anti‐RhoA (Abcam EP487Y 1:200), anti‐Rac1 (Abcam ab64533 1:150) and anti‐Cdc42 (cell division

(7) Ephrin-B2 paces neuronal production in the developing neocortexBMC developmental biologyMay 13, 2020Kischel A, Audouard C, Fawal MA, Davy A.Goat, AF467, WB: 1/1000. Eph A4: Cell signaling, Rabbit, 8793, WB: 1/2000. P-Eph B1–2: Abcam, Rabbit, ab61791, WB: 1/1000. P-Eph A4: Gift from Greenberg’s lab, Harvard medical school, Rabbit, WB: 1/1000. In situ hybridization

(8) Ligand-independent EphB1 signaling mediates TGF-β-activated CDH2 and promotes lung cancer cell invasion and migrationJournal of CancerJanuary 1, 2020Wang L, Peng Q, Sai B, Zheng L, Xu J, Yin N, Feng X, Xiang J.Reagents Antibodies to proteins were obtained from the following sources: EphB1 (#ab129103) and phos-EphB1 (#ab61791) for Western blot were purchased from Abcam; EphB1 (#AF542) for immunohistochemistry was purchased from

(9) Blood vessels guide Schwann cell migration in the adult demyelinated CNS through Eph/ephrin signalingActa neuropathologicaSeptember 1, 2019Garcia-Diaz B, Bachelin C, Coulpier F, Gerschenfeld G, Deboux C, Zujovic V, Charnay P, Topilko P, Baron-Van Evercooren A.Sigma); anti-EphB1 (1:50, SAB4500776, Sigma; anti-Eph receptor B1 + Eph receptor B2 (phospho Y594) (1:50, ab61791, Abcam); anti-Ki67 (1:100, 556003, BD Biosciences); anti-cleaved caspase3 (1:500, 9661S, Cell Signalling);

(10) Role of forward and reverse signaling in Eph receptor and ephrin mediated cell segregationExperimental cell researchAugust 1, 2019Wu Z, Ashlin TG, Xu Q, Wilkinson DG.and Gapdh from Thermo Fisher (371700, MA5-15738, respectively); phosphorylated EphB2-Y594 from Abcam (ab61791); ephrinB1/2/3 from Santa Cruz (sc-910). 4.4 Immunocytochemistry Cells were fixed for 15 min in 4%

(11) EphrinB/EphB forward signaling in Müller cells causes apoptosis of retinal ganglion cells by increasing tumor necrosis factor alpha production in rat experimental glaucomatous modelActa neuropathologica communicationsOctober 24, 2018Liu ST, Zhong SM, Li XY, Gao F, Li F, Zhang ML, Zhu K, Sun XH, Wang X, Miao Y, et al.(A5316, 1:10000, Sigma-Aldrich, St. Louis, MO, USA), polyclonal rabbit anti-phosphoY594 EphB1/EphB2 (ab61791, 1:500, Abcam, Cambridge, MA, USA), polyclonal goat anti-EphB1 (AF542, 1:500, R&D systems), monoclonal

(12) EphB2 receptor tyrosine kinase promotes hepatic fibrogenesis in mice via activation of hepatic stellate cellsScientific reportsFebruary 7, 2018Mimche PN, Lee CM, Mimche SM, Thapa M, Grakoui A, Henkemeyer M, Lamb TJ.following primary antibodies: Human/mouse EphB2 clone AF467 (1:50; R&D Systems), phospho-EphB1/EphB2Y594 ab61791 (1:200; Abcam), phospho-SMAD2/SMAD3 D27F4 (1:100; Cell signaling); α-SMA clone 1A4 (1:100, Sigma), CD140b

(13) Eph-ephrin signaling modulated by polymerization and condensation of receptorsProceedings of the National Academy of SciencesDecember 12, 2017Samuel Ojosnegros et al.transferred onto PVDF membranes, were probed with antibodies against anti-phosphoY594Eph receptor B1/B2 (ab61791, 1:500; Abcam) or actin (A1978, 1:5,000; Sigma) or anti-EphB2 (AF467, 1:2,000; R&D) (SI Appendix, Fig.

(14) The Wound Microenvironment Reprograms Schwann Cells to Invasive Mesenchymal-like Cells to Drive Peripheral Nerve RegenerationNeuronSeptember 27, 2017Clements MP, Byrne E, Camarillo Guerrero LF, Cattin AL, Zakka L, Ashraf A, Burden JJ, Khadayate S, Lloyd AC, Marguerat S, et al.protocols. Antibodies used for western blotting were as follows: pEphB1/B2, rabbit polyclonal (Abcam ab61791 1:1000); EphB2, goat polyclonal (R&D Systems AF467 1:2000); β-actin rabbit polyclonal (Abcam ab8227

(15) A Chemical-Genetic Approach to Generate Selective Covalent Inhibitors of Protein KinasesACS Chemical BiologyJune 16, 2017Alvin Kung, Marianne Schimpl, Arunika Ekanayake, Ying-Chu Chen, Ross Overman, Chao Zhangwere determined by a BCA assay (Pierce), normalized, and analyzed by Western blots for phospho-EphB (Ab61791, Abcam), EphB3 (Ab133742, Abcam), EphB1 (Ab129103, Abcam), β-actin (13E5, Cell-signaling), and EphA4

(16) Eph-mediated tyrosine phosphorylation of citron kinase controls abscissionThe Journal of cell biologyAugust 29, 2016Jungas T, Perchey RT, Fawal M, Callot C, Froment C, Burlet-Schiltz O, Besson A, Davy A.Cell Signaling Technology. Phalloidin (IF 1:200; ab176753) and anti–phospho EphB Y594 (rabbit, IF 1:200, WB 1:1,000; ab61791) were purchased from Abcam. Anti-CitK (goat, IF 1:200, WB 1:1,000; sc-1848), anti-Crik (mouse, BD, WB

(17) Exosomes mediate cell contact-independent ephrin-Eph signaling during axon guidanceThe Journal of cell biologyJuly 4, 2016Gong J, Körner R, Gaitanos L, Klein R.Santa Cruz Biotechnology, Inc.), anti-EphB2 (AF467, goat polyclonal; R&D Systems), anti-EphB1/B2 (phospho-Y594; ab61791, rabbit polyclonal; Abcam), M2 anti-FLAG (F3165, mouse monoclonal; Sigma-Aldrich), anti-FLAG (F7425

(18) Direct cell–cell contact with the vascular niche maintains quiescent neural stem cellsNature Cell BiologyNovember 1, 2014Cristina Ottone, Benjamin Krusche, Ariadne Whitby, Melanie Clements, Giorgia Quadrato, Mara E. Pitulescu, Ralf H. Adams, Simona Parrinellofollowing primary antibodies were used: rabbit antibodies to Sox2 (Abcam ab97959, 1:400), pEph (Abcam ab61791, 1:500), p-ERK (Cell Signalling 4370, 1:100), Ki67 (Novocastra 201701, 1:500), GFAP (DAKO Z0334, 1:1000),

(19) Overcoming EMT-associated resistance to anti-cancer drugs via Src/FAK pathway inhibitionOncotargetSeptember 15, 2014Catherine Wilson, Katrina Nicholes, Daisy Bustos, Eva Lin, Qinghua Song, Jean-Philippe Stephan, Donald S. Kirkpatrick, Jeff Settleman

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Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596) antibody from Abcam

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Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596) antibody from Abcam

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