Anti-Neutrophil antibody [7/4] (FITC) ab53453 from Abcam

Product Specs
ItemAnti-Neutrophil antibody [7/4] (FITC)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab53453
Antibody TypePrimary
Quantity50 UG
Conjugate/TagFITC
IsotypeIgG2a
ClonalityMonoclonal
HostRat
ApplicationsFlow Cytometry, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Clone43928
FormatPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Concentration0.1 mg/ml
Gene NameLy6b
ReactivityMouse
TargetNeutrophil

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) The neutrophil enzyme myeloperoxidase modulates neuronal response in a model of subarachnoid hemorrhage by venous injuryStrokeOctober 1, 2021Aminata P. Coulibaly et al.Laboratories, Hercules, CA, US), anti-CC1 (AB16794), anti-GFAP (AB4674), anti-neutrophil antibody 7/4-FITC (AB53453), and anti-vimentin (AB92547) (Abcam, Cambridge, MA, US), anti-Ki67-APC (50569882, Invitrogen, Carlsbad,

(2) Autophagy modulates endothelial junctions to restrain neutrophil diapedesis during inflammationImmunitySeptember 14, 2021Reglero-Real N, Pérez-Gutiérrez L, Yoshimura A, Rolas L, Garrido-Mesa J, Barkaway A, Pickworth C, Saleeb RS, Gonzalez-Nuñez M, Austin-Williams SN, et al.CD11b (clone M1/70),Biolegend,Cat#1012020; RRID: AB_312785 Anti-mouse neutrophil (clone 7/4),Abcam,Cat#ab53453; RRID: AB_881408 Anti-mouse CD16/CD32 (CLONE 2.4G2),BDBiosciences,Cat#553141; RRID: AB_394656 Anti-human

(3) Myeloid cell–targeted STAT3 inhibition sensitizes head and neck cancers to radiotherapy and T cell–mediated immunityJournal of Clinical InvestigationJanuary 19, 2021Dayson Moreira et al.CD206 (MR5D3), F4/80 (BM8), Ly6C (HK1.4), Ly6G (RB6-8C5) (all Thermo Fisher Scientific), and Ly6B.2 (ab53453, Abcam), after blocking with anti-FcγIII/IIR (2.4G2, Thermo Fisher Scientific). For intracellular staining,

(4) Neutrophil extracellular traps released by neutrophils impair revascularization and vascular remodeling after strokeNature communicationsMay 19, 2020Kang L, Yu H, Yang X, Zhu Y, Bai X, Wang R, Cao Y, Xu H, Luo H, Lu L, et al.LSRFortessaTM, BD Biosciences) using a FITC-conjugated rat monoclonal anti-mouse neutrophil antibody (anti-7/4; ab53453, Abcam) and analyzed using FlowJo V10 software (Tree Star, Inc., Ashland, OR). DNase 1 treatment Mice

(5) Endothelium-targeted deletion of the miR-15a/16-1 cluster ameliorates blood-brain barrier dysfunction in ischemic strokeScience signalingApril 7, 2020Ma F, Sun P, Zhang X, Hamblin MH, Yin KJ.following primary antibodies: mouse anti-F4/80 (BioLegend, Cat #123102), rat anti-Ly6B (Abcam, Cat #ab53453), rabbit anti-NeuN (EMD Millipore, Cat #ABN78), rabbit anti-Iba1 (Wako, Cat #019–19741), mouse anti-CD16/32

(6) Brain-to-cervical lymph node signaling after strokeNature communicationsNovember 22, 2019Esposito E, Ahn BJ, Shi J, Nakamura Y, Park JH, Mandeville ET, Yu Z, Chan SJ, Desai R, Hayakawa A, et al.Agilent), FITC anti-CD4 antibody (1:100, 561828, BD Biosciences), FITC Neutrophil antibody (1:100, ab53453, Abcam), PE anti-CD34 antibody (1:100, 551387, BD Biosciences), APC anti-F4/80 antibody (1:200, 123116,

(7) Tissue-Restricted Adaptive Type 2 Immunity Is Orchestrated by Expression of the Costimulatory Molecule OX40L on Group 2 Innate Lymphoid CellsImmunityJune 19, 2018Halim TYF, Rana BMJ, Walker JA, Kerscher B, Knolle MD, Jolin HE, Serrao EM, Haim-Vilmovsky L, Teichmann SA, Rodewald HR, et al.AB_313254 Anti-mouse MHCII,Thermo Fisher,48-5321-82; RRID: AB_1272204 Anti-mouse Ly6B (7/4),Abcam,Ab53453; RRID: AB_881408 Anti-mouse OX40,Biolegend,119409; RRID: AB_2272150 Anti-mouse OX40L,Thermo Fisher,12-5905-82;

(8) In vivo structural and cellular remodeling of engineered bone–ligament–bone constructs used for anterior cruciate ligament reconstruction in sheepConnective Tissue ResearchNovember 1, 2016Shelby E. Florida, Keith W. VanDusen, Vasudevan D. Mahalingam, Aleesa J. Schlientz, Edward M. Wojtys, Deneen M. Wellik, Lisa M. Larkin

(9) Tumour hypoxia promotes melanoma growth and metastasis via High Mobility Group Box-1 and M2-like macrophagesScientific reportsJuly 18, 2016Huber R, Meier B, Otsuka A, Fenini G, Satoh T, Gehrke S, Widmer D, Levesque MP, Mangana J, Kerl K, et al.

(10) DMSO Represses Inflammatory Cytokine Production from Human Blood Cells and Reduces Autoimmune ArthritisPloS oneJanuary 1, 2016Elisia I, Nakamura H, Lam V, Hofs E, Cederberg R, Cait J, Hughes MR, Lee L, Jia W, Adomat HH, et al.(ThermoFisher Scientific, Waltham, MA, USA), CD45.2-eFluor450 (clone 104 from ebioscience, San Diego, CA, USA), 7/4-FITC (ab53453 from Abcam, Toronto, ON, Canada) and Gr-1-PE (clone RB6-8C5 from AbLab.ca, Vancouver, BC) to identify

(11) Yeast virus-derived stimulator of the innate immune system augments the efficacy of virus vector-based immunotherapyJournal of virologyMay 1, 2014Claudepierre MC, Hortelano J, Schaedler E, Kleinpeter P, Geist M, Remy-Ziller C, Brandely R, Tosch C, Laruelle L, Jawhari A, et al.ultracentrifuged, and was purchased from Miltenyi Biotec (Germany), FITC-conjugated rat anti-mouse 7/4 (clone ab53453) was purchased from Abcam (United Kingdom), and APC-conjugated anti-mouse Langerin (clone 929F3.01) was

(12) B cell-specific loss of Lyn kinase leads to autoimmunityJournal of immunology (Baltimore, Md. : 1950)February 1, 2014Lamagna C, Hu Y, DeFranco AL, Lowell CA.

(13) VDR attenuates acute lung injury by blocking Ang-2-Tie-2 pathway and renin-angiotensin systemMolecular endocrinology (Baltimore, Md.)December 1, 2013Juan Kong, Xiangdong Zhu, Yongyan Shi, Tianjing Liu, Yunzi Chen, Ishir Bhan, Qun Zhao, Ravi Thadhani, Yan Chun Li

(14) Anthracyclines induce DNA damage response-mediated protection against severe sepsisImmunityNovember 14, 2013Figueiredo N, Chora A, Raquel H, Pejanovic N, Pereira P, Hartleben B, Neves-Costa A, Moita C, Pedroso D, Pinto A, et al.

(15) Production and Release of Antimicrobial and Immune Defense Proteins by Mammary Epithelial Cells following Streptococcus uberis Infection of SheepInfection and ImmunitySeptember 1, 2013Maria Filippa Addis, Salvatore Pisanu, Gavino Marogna, Tiziana Cubeddu, Daniela Pagnozzi, Carla Cacciotto, Franca Campesi, Giuseppe Schianchi, Stefano Rocca, Sergio Uzzau

(16) Myeloperoxidase-derived oxidants induce blood-brain barrier dysfunction in vitro and in vivoPloS oneJanuary 1, 2013Andreas Üllen, Evelin Singewald, Viktoria Konya, Günter Fauler, Helga Reicher, Christoph Nusshold, Astrid Hammer, Dagmar Kratky, Akos Heinemann, Peter Holzer, Ernst Malle, Wolfgang Sattler

(17) Immune cells from SR/CR mice induce the regression of established tumors in BALB/c and C57BL/6 micePloS oneJanuary 1, 2013Koch J, Hau J, Pravsgaard Christensen J, Elvang Jensen H, Bagge Hansen M, Rieneck K.-CD19, -F4/80 and NK1.1 (BD Pharmingen, San Diego, CA, USA), isotype controls and an anti-PMN antibody (ab53453, Abcam, Cambridge, UK). Cells from the non-depleted suspensions were prepared in parallel to assess

(18) Cathepsin G-dependent modulation of platelet thrombus formation in vivo by blood neutrophilsPloS oneJanuary 1, 2013Faraday N, Schunke K, Saleem S, Fu J, Wang B, Zhang J, Morrell C, Dore S.

(19) Simvastatin antagonizes CD40L secretion, CXC chemokine formation, and pulmonary infiltration of neutrophils in abdominal sepsisJournal of leukocyte biologyMay 1, 2011Zhang S, Rahman M, Zhang S, Qi Z, Thorlacius H.

(20) IL-17 produced by neutrophils regulates IFN-gamma-mediated neutrophil migration in mouse kidney ischemia-reperfusion injuryThe Journal of clinical investigationJanuary 1, 2010Li L, Huang L, Vergis AL, Ye H, Bajwa A, Narayan V, Strieter RM, Rosin DL, Okusa MD.

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Anti-Neutrophil antibody [7/4] (FITC) from Abcam

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Anti-Neutrophil antibody [7/4] (FITC) from Abcam

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