Fig 1: Development of a multispectral panel for the topological examination of DCs. (A) Representative images showing staining for CLEC9A (yellow), CD20 (cyan), CD11c (orange), CD123 (red), CD8 (pink), CD4 (green), Ki67 (magenta) and JOPRO-1 (blue) in a tonsillar tissue section and the distribution of each protein marker with respect to the MZ, LZ and DZ (middle panels). Red dotted square enclosures in lower middle panels denote the areas that are presented magnified in the lower middle panels. (B) Gating strategy used for the sequential positional immunophenotyping of CD4, CD8, CD20, CD11c+, CD11c+CLEC9A+CD123- and CD11c-CD123+CLEC9A- in GC (CD20dimKi67hi), F (CD20hi/dim) and EF (CD20lo) areas using histocytometry. (C) Box graphs and box plots showing the relative frequencies of CD11c+, CD11c+CLEC9A+CD123- and CD11c-CD123+CLEC9A- immune cells in tonsillar tissue as well as in CD20hi/dim areas (upper panels) and relative frequencies of CD20, CD4, CD8 and CD11c+ immune cells in nine individual follicles. Images were acquired at 40x (NA 1.3) with 1% magnification. Scale bars are 100um for all panels displaying an entire follicle), 20um in the middle panel close ups and 5um in the right panel single staining close ups.
Fig 2: Systemic effects of VPC23019 (S1P1/S1P3 antagonist) on obesity, glucose intolerance, and adipocyte hypertrophy/inflammation. A, Body weight change in wild-type (open symbols) and ob/ob (closed symbols) male mice fed the control diet (Con or C) or VPC23019 (40 mg/kg)-formulated diet (VPC or V) after age 10 weeks. B, Daily food intake calculated from 4 cycles of the sum values from 3 consecutive days between weeks 2 and 4 after food change. C, Epididymal fat weights at week 12. D, Intraperitoneal glucose tolerance test (ip-GTT) test in wild-type (left) and ob/ob (right) mice fed Con or VPC diet for 12 weeks. Areas under the curve (AUC) are presented in inset graphs. E to J, Serum levels of E, fasting blood glucose; F, fasting blood (immunoreactive) insulin; G, triacylglycerol; H, total cholesterol; I, aspartate transaminase (AST); and J, alanine transaminase (ALT) in wild-type and ob/ob mice fed the aforementioned diets for 12 weeks. K to N, Representative hematoxylin/eosin (HE)-stained image of K, epididymal and M, inguinal adipocytes and their cell area sizes (L and N, respectively) from wild-type and ob/ob mice. Bars: 100 µm. O, Immunochemical CD11c and CD206 staining of epididymal adipocyte sections prepared from ob/ob mice. The nuclei were stained with DAPI (4',6-diamidino-2-phenylindole). Bars: 50 µm. P, HE-stained hepatic sections prepared from ob/ob mice. Bars: 100 µm. Data are mean ± SD (n in parentheses) and the effects of VPC23019 were not statistically significant in any parameters.
Fig 3: Expression of adipocyte inflammation marker genes in epididymal and inguinal fats from wild-type and ob/ob mice fed the control, JTE-013, SEW-2871, or VPC23019 diet for 12 weeks. Messenger RNA levels of A and B, tumor necrosis factor a (Tnfa); C and D, CD11c (Cd11c); E and F, CD206 (Cd206); and G and H, adiponectin (Adipoq) in the A, C, E, and G, epididymal and B, D, F, and H, inguinal fats of wild-type and ob/ob mice were normalized by that of GAPDH (glyceraldehyde-3-phosphate dehydrogenase) and expressed with that of wild-type mice fed the same control diet as 1. Data are mean ± SD (n in parentheses); *P less than .05 and **P less than .01 vs wild-type samples (black asterisks) or control samples (red and blue asterisks for JTE-013 and SEW-2871 samples, respectively).
Fig 4: Effects of JTE-013 and SEW-2871 on adipocyte hypertrophy and inflammation in epididymal and inguinal fats of wild-type and ob/ob mice. A to D, Representative hematoxylin/eosin (HE)-stained image of cross-sectional A, epididymal and C, inguinal adipocytes and their cell area sizes (B and D, respectively) from wild-type and ob/ob mice fed the control diet (Con), JTE-013 diet (JTE), or SEW-2871 diet (SEW) for 12 weeks. The suppressive effects by JTE-013 or SEW-2871 are statistically significant at *P less than .05, **P less than .01, ***P less than .001 in t test. Bars: 100 µm. E, Immunochemical CD11c and CD206 staining of epididymal adipocytes isolated from ob/ob mice. The nuclei were stained with DAPI (4',6-diamidino-2-phenylindole). Bars: 50 µm. F, HE-stained hepatic sections isolated from ob/ob mice. Bars: 100 µm.
Supplier Page from Proteintech Group Inc for CD11c/Integrin Alpha X antibody