GFP Antibody 600-401-215 from Rockland Immunochemicals, Inc.

Product Specs
ItemGFP Antibody
CompanyRockland Immunochemicals, Inc.
Price Pricing Info Supplier Page View Company Product Page
Catalog Number600-401-215
Quantity100 µg
ClonalityPolyclonal
Conjugate/TagUnconjugated
HostRabbit
ImmunogenThe immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria
Concentration1.25 mg/mL
ApplicationsELISA,Flow Cytometry,Immunohistochemistry,IF Microscopy,Immunoprecipitation,Western Blot
PurityAnti-GFP antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). No reaction was observed against Human, Mouse or Rat serum proteins
FormatLiquid (sterile filtered)
ReactivityGFP, eGFP, rGFP, RS-GFP, S65T-GFP, YFP
TargetGFP
Antibody TypePrimary
StorageStore Anti-GFP Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. GFP antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use

Specifications/Features

Application Note: Anti-GFP antibody is designed to detect GFP and its variants. GFP antibody has been tested by western blot and ELISA. This product can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with Rockland's monoclonal anti-GFP (600-301-215) using either form of the antibody as the capture or detection antibodies. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin conjugated HRP (code # S000-03). Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and can detect GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.

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Rockland Immunochemicals, Inc.

P.O. Box 5199

Limerick, Pennsylvania 19468

United States

Phone: (484) 791-3823
Fax: (484) 369-8654

Company Profile

Website: https://www.rockland.com

(1) Site-specific phosphorylations of the Arf activator GBF1 differentially regulate GBF1 function in Golgi homeostasis and secretion versus cytokinesisSci RepAugust 21, 2023Kendall Walton, Tomasz J. Nawara, Allyson R. Angermeier, Hadley Rosengrant, Eunjoo Lee, Bridge Wynn, Ekaterina Victorova, George Belov, Elizabeth SztulBD Bioscience (San Jose, CA). Rabbit polyclonal anti-RFP (600-401-379) and rabbit polyclonal anti-GFP (600-401-215) were purchased from Rockland (Philadelphia, PA). Mouse monoclonal anti-acetylated tubulin (T6793) was

(2) Protocol to determine the subcellular localization of protein interactions in murine keratinocytesSTAR ProtocolsJune 1, 2023Lisa MüllerRESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, GFP rabbit polyclonal antibody,Rockland,Cat#600-401-215; RRID: AB_828167 "Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mouse monoclonal antibody (1:1,000)",Sigma-Aldrich,Cat#G8795;

(3) Bulk and mosaic deletions of Egfr reveal regionally defined gliogenesis in the developing mouse forebrainiScienceMarch 17, 2023Zhang X, Xiao G, Johnson C, Cai Y, Horowitz ZK, Mennicke C, Coffey R, Haider M, Threadgill D, Eliscu R, et al.anti-GFP (1:200),Aves Labs,Cat# GFP-1020; RRID: AB_2307313 Rabbit anti-RFP,Rockland Immunochemicals,Cat# 600-401-215; RRID: AB_828167 AlexaFluor Donkey anti-Chicken 647 (1:200),Jackson ImmunoResearch,Cat# 703-605-155;

(4) The fidelity of transcription in human cellsProceedings of the National Academy of SciencesJanuary 31, 2023Claire Chung, Bert M. Verheijen, Xinmin Zhang, Biao Huang, Aeowynn Coakley, Eric McGann, Emily Wade, Olivia Dinep-Schneider, Jessica LaGosh, Maria-Eleni Anagnostou, et al.Scientific). For immunofluorescence, sections were incubated with rabbit anti-GFP primary antibodies (1:500, 600-401-215, Rockland, USA) overnight at 4 °C. Next, sections were rinsed with PBS and incubated with Alexa Fluor

(5) The putative transporter MtUMAMIT14 participates in nodule formation in Medicago truncatulaScientific ReportsJanuary 16, 2023Kevin Garcia, Kaylee Cloghessy, Danielle R. Cooney, Brett Shelley, Sanhita Chakraborty, Arjun Kafle, Aymeric Busidan, Unnati Sonawala, Ray Collier, Dhileepkumar Jayaraman, et al.nitrocellulose membrane, proteins were detected using an anti-GFP primary antibody (1:5000; Rockland, CAT 600–401-215) and a goat-anti-rabbit secondary antibody (1:5000, BioRad), by chemiluminescence. Statistical analyses Data

(6) Cullin 4b-RING ubiquitin ligase targets IRGM1 to regulate Wnt signaling and intestinal homeostasisCell death and differentiationSeptember 1, 2022Fan Y, Huo X, Guo B, Zhang X, Yang Y, Lian J, Meng X, Shao Y, Zou Y, Guo H, et al.

(7) Inducible and tissue-specific cell labeling in Cre-ER^T2 transgenic Xenopus linesDevelopment, growth & differentiationJune 1, 2022Lin TY, Taniguchi-Sugiura Y, Murawala P, Hermann S, Tanaka EM.Before immunostaining, slides were thawed and stained with primary antibodies against GFP (Rockland, 600‐401‐215, 1:1000, and MPI‐CBG, Dresden, 1:1000), mCherry (Acris Antibodies, AB0040‐200, 1:1000), Prrx1 (Gerber

(8) Astrocyte-targeted gene delivery of interleukin 2 specifically increases brain-resident regulatory T cell numbers and protects against pathological neuroinflammationNature immunologyJune 1, 2022Yshii L, Pasciuto E, Bielefeld P, Mascali L, Lemaitre P, Marino M, Dooley J, Kouser L, Verschoren S, Lagou V, et al.(1:500 dilution; ABN90P, Millipore), GFP (1:300 dilution, 132002, Synaptic Systems; 1:1,000 dilution, 600-401-215, Rockland; 1:500 dilution, 600-101-215, Rockland), GFAP (1:1,000 dilution; 173004, Synaptic Systems),

(9) Twist regulates Yorkie activity to guide lineage reprogramming of syncytial alary musclesCell reportsJanuary 25, 2022Rose M, Domsch K, Bartle-Schultheis J, Reim I, Schaub C.

(10) Transfected plasmid DNA is incorporated into the nucleus via nuclear envelope reformation at telophaseCommunications biologyJanuary 20, 2022Haraguchi T, Koujin T, Shindo T, Bilir Ş, Osakada H, Nishimura K, Hirano Y, Asakawa H, Mori C, Kobayashi S, et al.5a. Primary rabbit polyclonal antibodies included anti-GFP antibody for iCLEM (1:200 dilution, Cat No. 600-401-215, Lot No. 24437, Rockland, Limerick, PA, USA), anti-emerin for IF (1:300 dilution, ED180) in Figs. 1b,

(11) Virally Mediated Connexin 26 Expression in Postnatal Scala Media Significantly and Transiently Preserves Hearing in Connexin 30 Null MiceFrontiers in cell and developmental biologyJanuary 1, 2022Zhang L, Wang W, Kim SM, Wang J, Zhou B, Kong W, Zheng J, Lin X.(1:200, Proteus biosciences Inc., 25–6,790, Ramona, CA) or GFP (1:200, Rockland Immunochemicals Inc., 600–401–215, Limerick, PA) diluted with 0.1% PBST at 4°C overnight. After washing three times with PBS (10 min/each

(12) The CRL4DTL E3 ligase induces degradation of the DNA replication initiation factor TICRR/TRESLIN specifically during S phaseNucleic acids researchOctober 11, 2021Wittig KA, Sansam CG, Noble TD, Goins D, Sansam CL.

(13) Metabolic control of T_FH cells and humoral immunity by phosphatidylethanolamineNatureJuly 1, 2021Fu G, Guy CS, Chapman NM, Palacios G, Wei J, Zhou P, Long L, Wang YD, Qian C, Dhungana Y, et al.containing the following antibodies or lectins where indicated: rabbit anti-GFP/Ametrine (Rockland; catalog 600–401-215), anti-IgD (Biolegend; clone 11–26c.2a), anti-CD3 (Biolegend; clone 17A2), or biotin-peanut agglutinin

(14) Integrated sensing of host stresses by inhibition of a cytoplasmic two-component system controls M. tuberculosis acute lung infectioneLifeMay 18, 2021Buglino JA, Sankhe GD, Lazar N, Bean JM, Glickman MS.

(15) Fibroblast dedifferentiation as a determinant of successful regenerationDevelopmental cellMay 17, 2021Lin TY, Gerber T, Taniguchi-Sugiura Y, Murawala P, Hermann S, Grosser L, Shibata E, Treutlein B, Tanaka EM.immunostaining, slides were thawed and were stained with primary antibodies against GFP (Rockland, 600-401-215, 1:1000, and MPI-CBG, Dresden, 1:1000), mCherry (Acris Antibodies, AB0040-200, 1:1000), Sox9 (Chemicon,

(16) Genetic analysis of the septal peptidoglycan synthase FtsWI complex supports a conserved activation mechanism for SEDS-bPBP complexesPLoS geneticsApril 1, 2021Li Y, Gong H, Zhan R, Ouyang S, Park KT, Lutkenhaus J, Du S.

(17) Intravital quantification reveals dynamic calcium concentration changes across B cell differentiation stageseLifeMarch 22, 2021Ulbricht C, Leben R, Rakhymzhan A, Kirchhoff F, Nitschke L, Radbruch H, Niesner RA, Hauser AE.jaxmice, Antibody,Rabbit anti-GFP-Alexa488 polyclonal,"Rockland, in-house coupling DRFZ Berlin",Cat# 600-401-215,1:200 Antibody,Rat anti-CD21/35-Fab-Atto590 7G6,DRFZ Berlin,–,10 µg/mouse Antibody,Hamster anti-IgD-Alexa594

(18) Cytoneme delivery of Sonic Hedgehog from ligand-producing cells requires Myosin 10 and a Dispatched-BOC/CDON co-receptor complexeLifeFebruary 11, 2021Hall ET, Dillard ME, Stewart DP, Zhang Y, Wagner B, Levine RM, Pruett-Miller SM, Sykes A, Temirov J, Cheney RE, et al.(IF) antibody,anti-OLIG2 (Rabbit polyclonal),Millipore,AB9610,1:300 (IF) antibody,anti-GFP (Rabbit polyclonal),Rockland,"600-401-215, RRID:AB_828167",1:8000 (WB) antibody,anti-Kif5b (Rabbit monoclonal),Abcam,"ab167429, RRID:AB_2715530",1:5000

(19) Regulatory inter-domain interactions influence Hsp70 recruitment to the DnaJB8 chaperoneNature communicationsFebruary 11, 2021Ryder BD, Matlahov I, Bali S, Vaquer-Alicea J, van der Wel PCA, Joachimiak LA.temperature. For immunolabelling, we added 1:2000 dilution of polyclonal anti-GFP (rabbit) (Rockland; 600-401-215; 35460) or anti-DnaJB8 (rabbit) (Abcam; ab235546; GR3229943-2) in milk and incubated the membrane shaking

(20) MCT1 Deletion in Oligodendrocyte Lineage Cells Causes Late-Onset Hypomyelination and Axonal DegenerationCell reportsJanuary 12, 2021Philips T, Mironova YA, Jouroukhin Y, Chew J, Vidensky S, Farah MH, Pletnikov MV, Bergles DE, Morrison BM, Rothstein JD.anti-GFAP,MilliporeSigma,Cat#AB5541; RRID:AB_10579068 rabbit polyclonal anti-GFP,Rockland Immunochemicals,Cat#600–401-215; RRID:AB_828167 rabbit polyclonal anti-Glut1,Abcam,Cat#Ab652; RRID:AB_305540 rabbit monoclonal anti-Glut3,Abcam,Cat#Ab191071;

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9/10/2015GFP Antibody That Works Really Well Ran GThe purpose of our experiment was to study the expression of cytokine receptor GFP knock-in by immunofluorescence. GFP detection otherwise is sensitive to bleaching, but use of this antibody enhances ... Read Review

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GFP Antibody

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GFP Antibody from Rockland Immunochemicals, Inc.

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Rockland Immunochemicals, Inc.

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GFP Antibody from Rockland Immunochemicals, Inc.

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