Fig 2: S100ß and NDRG2 immunoreactivity in the rat brain. a (merged) Neocortex layer III; most labeled cells (asterisk) are immunoreactive for both antigens (yellow). b (red channel) Corpus callosum; NDRG2 expression in some cells is relatively weak (arrow). c Green channel (corresponding to b): The cell that is also indicated in (b) (arrow) shows a pronounced S100ß expression; arrowhead denotes S100ß immunoreactive fiber. d (merged) The cell that is also indicated in (b and c) shows strong S100ß but weak NDRG2 immunoreactivity; arrowhead denotes S100ß immunoreactive fiber that does not contain NDRG2

Fig 3: Glia cells in rat cerebellum and hippocampus. a (merged) Cerebellar Purkinje cell layer (Pcl); note the strongly NDRG2 immunoreactive Bergmann glia cell bodies (asterisk) that are located between the unlabeled Purkinje neurons (Pkj); Bergmann glial fibers (Bgf; red) in the molecular layer (ml) are associated with the glutamatergic parallel fibers (green); gcl granule cell layer. b (merged) Layer of granule cells (gc) in the cerebellum; note the NDRG2 process (arrow), which is associated with a VGLUT1 immunoreactive mossy fiber terminal (mft). c (merged) Hippocampal stratum lucidum; note the fine NDRG2 positive process (arrow), which is associated with the giant VGLUT1 immunoreactive mossy fiber terminal (mft) that synapses on dendrites (D) of pyramidal neurons (Pyr). d (merged) Hippocampal stratum lucidum; note that fine NDRG2 positive processes are associated with VGAT immunoreactive nerve terminals; asterisk indicates NDRG2 immunoreactive cell body

Fig 4: Overview of NDRG2 and GFAP immunoreactivity in brains of mouse, rat and marmoset. Sections from the level of the hippocampus were immunohistochemically processed for light microscopy. a Mouse, NDRG2 immunoreactivity; the inset shows hippocampal area CA1 at a higher magnification. b Mouse, GFAP immunoreactivity; note that NDRG2 immunoreactivity (a) is more evenly distributed throughout gray matter areas than GFAP immunoreactivity (b). c Rat, NDRG2 immunoreactivity. d Marmoset, NDRG2 immunoreactivity. CA1, CA3 cornu ammonis regions 1 and 3; CC corpus callosum; DG dentate gyrus; hil hilus; Ncx neocortex. Calibration bars 200 µm

Fig 5: NDRG2 (red) and GFAP (green) immunoreactivity in marmoset (a, b, d) and tree shrew (c). a Caudate nucleus; note the strong NDRG2 immunoreactivity of the astrocytic cell body (asterisk) and the array of fine fibers in the surroundings of the cell; arrow denotes a GFAP immunoreactive fiber. b Cortical layer I; only a few cell bodies (asterisk) display NDRG2 immunoreactivity whereas GFAP-positive fibers (arrow) are abundant. c Cortical layer III; an immunoreactive NDRG2-positive astrocyte (asterisk) is located close to a blood vessel (BV). d Border between corpus callosum (CC) and caudate nucleus (CD); note that in the CC, there are cells stained for both antigens (asterisk) as well as cells in which GFAP dominates (arrow head). Very low GFAP immunoreactivity is found in the CD