Anti-Vimentin antibody ab24525 from Abcam

Product Specs
ItemAnti-Vimentin antibody
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab24525
HostChicken
Quantity50 UL
ClonalityPolyclonal
IsotypeIgY
ApplicationsImmunocytochemistry/ Immunofluorescence, Immunohistochemistry (PFA perfusion fixed frozen sections), Western blot
Concentration20 mg/ml
Gene NameVIM
ReactivityHuman, Mouse, Rat
TargetVimentin
Conjugate/TagUnconjugated
Antibody TypePrimary

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Time- and Concentration-Dependent Adverse Effects of Paclitaxel on Non-Neuronal Cells in Rat Primary Dorsal Root GangliaToxicsJuly 4, 2023Amira Elfarnawany, Faramarz Dehghanifollowed as described in Section 2.8.3. Chicken anti-vimentin polyclonal primary antibody (Abcam, cat No. ab24525, Cambridge, UK, 1:1000) combined with goat anti-chicken IgY Alexa Fluor® 488 conjugated (Invitrogen,

(2) Developmental Expression of the Cell Cycle Regulator p16INK4a in Retinal Glial Cells: A Novel Marker for Immature Ocular Astrocytes?Journal of Histochemistry & CytochemistryJune 23, 2023Cristina Martinez-Fernandez Martinez-Fernandez de la Camara, Tina Storm, Ahmed Salman, Thomas Burgoyne, Martin Qvist Rasmussen, Harry O. Orlans, Angela J. Russell, Stephen G. Davies, Alun R. Barnard, Robert E. MacLarenpolyclonal anti-GFAP (1:2000) (ab4674; Abcam, Cambridge, UK), chicken polyclonal anti-vimentin (1:1000) (ab24525; Abcam, Cambridge, UK), rabbit polyclonal anti-PAX2 (1:200) (71–6000; Invitrogen, Inchinnan, Scotland,

(3) Lung mesenchymal cells from patients with COVID-19 driven lung fibrosis: Several features with CTD-ILD derived cells but with higher response to fibrogenic signals and might be more pro-inflammatoryBiomedicine & PharmacotherapyJune 1, 2023Sara Bozzini, Matteo Della Zoppa, Cecilia Bagnera, Eleonora Bozza, Stefania Croce, Chiara Valsecchi, Mirko Belliato, Laura Pandolfi, Patrizia Morbini, Patrizia Comoli, et al.BSA; overnight at 4 °C or 2 h at room temperature): anti-α-SMA [E184] (ab32575, Abcam), anti-Vimentin (ab24525, Abcam), anti-Col1a1 (ab34710, Abcam) and anti-β-Actin (MAB1501R, Chemicon). After wash, the membranes

(4) Cell Biology of Spontaneous Persistent Epithelial Defects After Photorefractive Keratectomy in RabbitsTranslational Vision Science & TechnologyMay 15, 2023Lycia Pedral Sampaio, Valeria Villabona Martinez, Thomas Michael Shiju, Guilherme S. L. Hilgert, Marcony R. Santhiago, Steven E. WilsonAntibodies Antigen,Company/Source,Species,Ab Isotype,Catalog No.,Dilution Vimentin,Abcam,Chicken,IgY,ab24525,1:2000 Keratocan,Winston Kao,Goat,IgG,—,1:200 α-SMA,Dako,Mouse,IgG2a,M0851,1:400 TGF-β1,Genetex,Mouse,IgG1,GTX21279,1:100 Collagen

(5) Engineered cardiac tissue model of restrictive cardiomyopathy for drug discoveryCell reports. MedicineMarch 21, 2023Wang BZ, Nash TR, Zhang X, Rao J, Abriola L, Kim Y, Zakharov S, Kim M, Luo LJ, Morsink M, et al.RRID:AB_2751827 Cardiac troponin (TNNT2),ThermoFisher,Cat# MA5-12960; RRID:AB_11000742 Vimentin,Abcam,Cat# Ab24525; RRID:AB_778824 Anti-chicken 594 secondary antibody,Thermofisher,Cat# A-11042; RRID:AB_2534099 Anti-mouse

(6) Cardiogenic control of affective behavioural stateNatureMarch 9, 2023Brian Hsueh, Ritchie Chen, YoungJu Jo, Daniel Tang, Misha Raffiee, Yoon Seok Kim, Masatoshi Inoue, Sawyer Randles, Charu Ramakrishnan, Sneha Patel, et al.followed by primary antibody staining overnight at 4 °C using the following antibodies: anti-vimentin (ab24525), anti-cardiac troponin I (ab188877) or anti-PGP9.5 (ab108986), purchased from Abcam at 1:200 dilution

(7) MicroMagnify: a multiplexed expansion microscopy method for pathogens and infected tissuesResearch squareMarch 6, 2023Zhao Y, Cheng Z, Stefani C, Skillman T, Klimas A, Lee A, DiBernardo E, Mueller Brown K, Milman T, Gallagher B, et al.at RT: rabbit polyclonal anti-α-tubulin (11224–1-AP, Proteintech), chicken polyclonal anti-vimentin (ab24525, Abcam), Chicken polyclonal anti-GFP (ab13970, Abcam), rabbit polyclonal anti-NEDD4 (PA5–17463, Invitrogen),

(8) Fibrotic remodeling and tissue regeneration mechanisms define the therapeutic potential of human muscular progenitorsBioengineering & translational medicineMarch 1, 2023Hsiao YC, Wang IH, Yang TL.respectively. The information of primary antibodies used in current study was listed as following: vimentin (ab24525, Abcam), α‐SMA (A2547, Sigma), collagen I (C2456, Sigma), FN1 (ED‐A) (ab6328, Abcam), MMP1 (ab137332,

(9) Salmonella effector SopB reorganizes cytoskeletal vimentin to maintain replication vacuoles for efficient infectionNature CommunicationsJanuary 30, 2023Shuangshuang Zhao, Qiuping Xu, Yanqin Cui, Su Yao, Sihui Jin, Qian Zhang, Zeyu Wen, Haihua Ruan, Xin Liang, Yanjie Chao, et al.D21H3 antibody (dilution 1:100; #5741, CST, US); vimentin chicken polyclonal antibody (dilution 1:1000; ab24525, Abcam, UK); LAMP1 rabbit monoclonal D2D11antibody (dilution 1:100; #9091, CST, US). Both primary and

(10) Tetra-color superresolution microscopy based on excitation spectral demixingLight, science & applicationsJanuary 2, 2023Wu W, Luo S, Fan C, Yang T, Zhang S, Meng W, Xu T, Ji W, Gu L.described in the Microtubule samples section. The primary antibody used was chicken anti-vimentin (ab24525, Abcam, used at 1:200 dilution). Secondary antibodies labeled with Alexa Fluor 647 (A21449, Invitrogen,

(11) Recapitulating porcine cardiac development in vitro: from expanded potential stem cell to embryo culture modelsFrontiers in cell and developmental biologyJanuary 1, 2023Rawat H, Kornherr J, Zawada D, Bakhshiyeva S, Kupatt C, Laugwitz KL, Bähr A, Dorn T, Moretti A, Nowak-Imialek M.,Stemcell Technologies,60062FI.1,1:100 TBX5,Rabbit,Sigma-Aldrich®,HPA064683,1:100 VE-CADHERIN,Mouse,Invitrogen,14-1449-82,1:100 VIMENTIN,Chicken,Abcam,ab24525,1:400 WT1,Rabbit,Abcam,ab89901,1:100 ZO-1,Mouse,Thermo Fischer,33-91010,1:100 Primary antibodies

(12) Expression of Wilms’ Tumor 1 Antigen, Vimentin, and Corticotropin-Releasing Factor in the Human Kidney with Focal Segmental Glomerulosclerosis and Effect of Oxidative Stress on These Markers in HEK 293 CellsKidney and Blood Pressure ResearchJanuary 1, 2023Roland Csurgyók, Gábor Sütő, István Wittmann, Tibor Vasmonoclonal antibody, Abnova, CAT number: MAB14650; vimentin polyclonal antibody, Abcam, CAT number: ab24525; and CRF polyclonal antibody, Bioss, CAT number: bs-0246R) in PBS for 60 min followed by three washes

(13) Rhodopsin-positive cell production by intravitreal injection of small molecule compounds in mouse models of retinal degenerationPloS oneJanuary 1, 2023Fujii Y, Arima M, Murakami Y, Sonoda KH.temperature. Cells were then incubated with primary antibodies (chicken anti-vimentin [polyclonal, 1/1000, ab24525, Abcam, Cambridge, UK], rabbit anti-glutamine synthetase [GS] [polyclonal, 1/100, ab73593, Abcam], mouse

(14) Hypothalamic remodeling of thyroid hormone signaling during hibernation in the arctic ground squirrelCommunications BiologyDecember 1, 2022Helen E. Chmura, Cassandra Duncan, Ben Saer, Jeanette T. Moore, Brian M. Barnes, C. Loren Buck, Helen C. Christian, Andrew S. I. Loudon, Cory T. WilliamsUK) to block non-specific binding sites before sequential incubation with 1:500 vimentin antibody (#ab24525, Abcam, Cambridge, UK) followed by 15 nM protein A gold (diluted 1:60 in PBS/ EA; British Biocell; Cardiff,

(15) FLIM for Evaluation of Difference in Metabolic Status between Native and Differentiated from iPSCs Dermal Papilla CellsCellsSeptember 1, 2022Alena Kashirina, Alena Gavrina, Artem Mozherov, Dmitriy Kozlov, Daria Kuznetsova, Ekaterina Vorotelyak, Elena Zagaynova, Ekaterina Kalabusheva, Aleksandra Kashina1:100); anti-αSMA (Abcam, Cambridge, UK (cat #ab5694), 1:200); anti-vimentin (Abcam, Cambridge, UK (cat #ab24525), 1:300); anti-fibronectin (Abcam, Cambridge, UK (cat #ab2413), 1:200); anti-versican (R&D systems,

(16) Basal extrusion of singleâ€oncogenic mutant cells induces domeâ€like structures with altered microenvironmentsCancer ScienceAugust 30, 2022Takanobu Shirai, Miho Sekai, Kei Kozawa, Nanami Sato, Nobuyuki Tanimura, Shunsuke Kon, Tomohiro Matsumoto, Takeru Murakami, Shoko Ito, Andrew Tilstonâ€Lunel, et al.rabbit anti‐Ki‐67 (ab16667), rabbit anti‐mannose receptor (CD206) (ab64693), chicken anti‐vimentin (ab24525), and fluorescein isothiocyanate (FITC)‐conjugated goat anti‐GFP (ab6662) antibodies were obtained from

(17) Novel 3D-Printed Cell Culture Inserts for Air–Liquid Interface Cell CultureLife (Basel)August 10, 2022Magdalena Bauer, Magdalena Metzger, Marvin Corea, Barbara Schädl, Johannes Grillari, Peter DungelKGaA, Darmstadt, Germany) for 1 h at room temperature. The primary antibodies, keratin 14 (K14) (1:200, ab24525, Abcam, Cambridge, UK), keratin 10 (K10) (1:400, GPK10, Progen, Heidelberg, Germany), and vimentin (1:1000,

(18) α-Catenin links integrin adhesions to F-actin to regulate ECM mechanosensing and rigidity dependenceJournal of Cell BiologyAugust 1, 2022Abhishek Mukherjee, Shay Melamed, Hana Damouny-Khoury, Malak Amer, Lea Feld, Elisabeth Nadjar-Boger, Michael P. Sheetz, Haguy Wolfensonfrom Benny Geiger’s lab, generated in-house [Chorev et al., 2014]), chicken anti-vimentin (1:500, #ab24525; Abcam), rabbit anti-N-cadherin (1:200, #ab18203; Abcam), rabbit anti-β-catenin (1:200, #C2206; Sigma-Aldrich)

(19) CX3CL1 Action on Microglia Protects from Diet-Induced Obesity by Restoring POMC Neuronal Excitability and Melanocortin System Activity Impaired by High-Fat Diet FeedingInternational journal of molecular sciencesJune 7, 2022Banerjee J, Dorfman MD, Fasnacht R, Douglass JD, Wyse-Jackson AC, Barria A, Thaler JP.Cat#C9205), mouse anti-NeuN (1:1000; Millipore Sigma Cat#MAB377) or chicken anti-Vimentin (1:5000; Abcam Cat#ab24525). After primary incubation overnight at 4 °C, all wells were processed for 1h with appropriate fluorescent

(20) Senolytic elimination of Cox2-expressing senescent cells inhibits the growth of premalignant pancreatic lesionsGutFebruary 1, 2022Kolodkin-Gal D, Roitman L, Ovadya Y, Azazmeh N, Assouline B, Schlesinger Y, Kalifa R, Horwitz S, Khalatnik Y, Hochner-Ger A, et al.III), CC3 (Cell signalling 9661), CD45.2 (Biolegend 109802), p53 (Santa Cruz Fl393), Vimentin (Abcam ab24525), F4/80 (Biolegend 123109) and Dclk1 (Abcam ab31704). For SA-βgal stains 10 µm Frozen tissue sections

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1/11/2016Mouse Skin Vimentin Immunofluorescence Staining Lin ChenThis polyclonal chicken anti vimentin antibody works well to stain fibroblasts in mouse skin. Less background compared to other tested antibodies.

Anti-Vimentin antibody from Abcam

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Anti-Vimentin antibody from Abcam

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