GOAT ANTI MOUSE IgG

Published customer image:
Texas red conjugated Goat anti Mouse IgG antibody (103007) used for the detection of bound anti CD80 antibody by immunofluorescence
Image caption:
Immunolabelling of Mφ by the monocyte/Mφ marker CD14 and the M1 Mφ marker CD80. Mφ were seeded at a density of 5.7×10⁴ cells/cm², with (coculture) or without the same number of MDA-MB-231 cells. In each case, the culture medium was replaced daily. 5 days later, all cultures were treated with 1 μg/ml LPS and 5 ng/ml IFNγ for 24 h before washing and labelling. CD14 fluorescent labelling is green, CD80 is red. Panel A: without TM, it can be seen that CD80 was expressed on activated Mφ cultured alone and was not detectable on Mφ in the cocultures. Panel B: in presence of TM, no CD80-positive cells were found in any of the cultures.

From: Joimel U, Gest C, Soria J, Pritchard LL, Alexandre J, Laurent M, Blot E, Cazin L, Vannier JP, Varin R, Li H, Soria C.
Stimulation of angiogenesis resulting from cooperation between macrophages and MDA-MB-231 breast cancer cells: proposed molecular mechanism and effect of tetrathiomolybdate.
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This image is from an open access article distributed under terms of a.

Published customer image:
Texas red conjugated Goat anti Mouse IgG antibody (103007) used for the detection of bound anti CD80 antibody by immunofluorescence
Image caption:
Immunolabelling of Mφ by the monocyte/Mφ marker CD14 and the M1 Mφ marker CD80. Mφ were seeded at a density of 5.7×10⁴ cells/cm², with (coculture) or without the same number of MDA-MB-231 cells. In each case, the culture medium was replaced daily. 5 days later, all cultures were treated with 1 μg/ml LPS and 5 ng/ml IFNγ for 24 h before washing and labelling. CD14 fluorescent labelling is green, CD80 is red. Panel A: without TM, it can be seen that CD80 was expressed on activated Mφ cultured alone and was not detectable on Mφ in the cocultures. Panel B: in presence of TM, no CD80-positive cells were found in any of the cultures.

From: Joimel U, Gest C, Soria J, Pritchard LL, Alexandre J, Laurent M, Blot E, Cazin L, Vannier JP, Varin R, Li H, Soria C.
Stimulation of angiogenesis resulting from cooperation between macrophages and MDA-MB-231 breast cancer cells: proposed molecular mechanism and effect of tetrathiomolybdate.
BMC Cancer. 2010 Jul 17;10:375.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.